BMRBj - BMREntryMaster

Found 1 Document (2.095 seconds)

BMRB: 6869

2AYA

Solution Structure of C-Terminal 14 kDa Domain of the tau subunit from Escherichia coli DNA Polymerase III

Authors

Jergic, S., Dixon, N.E., Otting, G., Su, X.C.

Assembly

DNA polymerase III subunit tau

Entity

1. DNA polymerase III subunit tau (polymer, Thiol state: all free), 128 monomers, 14334.04 Da Detail

MKALEHEKTP ELAAKLAAEA IERDPWAAQV SQLSLPKLVE QVALNAWKEE SDNAVCLHLR SSQRHLNNRG AQQKLAEALS MLKGSTVELT IVEDDNPAVR TPLEWRQAIY EEKLAQARES IIADNNIQ

Formula weight

14334.04 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.7 %, Completeness (bb): 97.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.7 % (1376 of 1485)	95.2 % (739 of 776)	88.5 % (502 of 567)	95.1 % (135 of 142)
Backbone	97.5 % (739 of 758)	98.0 % (248 of 253)	97.4 % (372 of 382)	96.7 % (119 of 123)
Sidechain	89.3 % (762 of 853)	93.9 % (491 of 523)	82.0 % (255 of 311)	84.2 % (16 of 19)
Aromatic	48.2 % (27 of 56)	85.7 % (24 of 28)	0.0 % (0 of 25)	100.0 % (3 of 3)
Methyl	96.3 % (156 of 162)	100.0 % (81 of 81)	92.6 % (75 of 81)

1. DNA polymerase III subunit tau

MKALEHEKTP ELAAKLAAEA IERDPWAAQV SQLSLPKLVE QVALNAWKEE SDNAVCLHLR SSQRHLNNRG AQQKLAEALS MLKGSTVELT IVEDDNPAVR TPLEWRQAIY EEKLAQARES IIADNNIQ

Show sample condition

Sample

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	DNA polymerase III subunit tau	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		5 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.32 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2AYA, Strand ID: A Detail

Release date

2007-03-27

Citation

The unstructured C-terminus of the tau subunit of Escherichia coli DNA polymerase III holoenzyme is the site of interaction with the alpha subunit
Jergic, S., Ozawa, K., Williams, N.K., Su, X.C., Scott, D.D., Hamdan, S.M., Crowther, J.A., Otting, G., Dixon, N.E.
Nucleic Acids Res. (2007), 35, 2813-2824, PubMed 17355988 , DOI 10.1093/nar/gkm079 , Abstract

Related entities 1. DNA polymerase III subunit tau, : 1 : 6 : 2 entities Detail

Interaction partners 1. DNA polymerase III subunit tau, : 35 interactors Detail

More details for 35 interactors identified by 10 citations

Experiments performed 10 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	DNA polymerase III subunit tau	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		5 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	DNA polymerase III subunit tau	[U-10% 13C]	0.6 mM
7	phosphate buffer		5 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
11	DNA polymerase III subunit tau	1.8 mM
12	phosphate buffer	5 mM
13	NaCl	100 mM
14	H2O	90 %
15	D2O	10 %

Sample #4

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
16	DNA polymerase III subunit tau	1.8 mM
17	phosphate buffer	5 mM
18	NaCl	100 mM
19	D2O	100 %

2 HNCACB

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	DNA polymerase III subunit tau	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		5 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	DNA polymerase III subunit tau	[U-10% 13C]	0.6 mM
7	phosphate buffer		5 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
11	DNA polymerase III subunit tau	1.8 mM
12	phosphate buffer	5 mM
13	NaCl	100 mM
14	H2O	90 %
15	D2O	10 %

Sample #4

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
16	DNA polymerase III subunit tau	1.8 mM
17	phosphate buffer	5 mM
18	NaCl	100 mM
19	D2O	100 %

3 CACB(CO)NH

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	DNA polymerase III subunit tau	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		5 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	DNA polymerase III subunit tau	[U-10% 13C]	0.6 mM
7	phosphate buffer		5 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
11	DNA polymerase III subunit tau	1.8 mM
12	phosphate buffer	5 mM
13	NaCl	100 mM
14	H2O	90 %
15	D2O	10 %

Sample #4

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
16	DNA polymerase III subunit tau	1.8 mM
17	phosphate buffer	5 mM
18	NaCl	100 mM
19	D2O	100 %

4 HNCO

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	DNA polymerase III subunit tau	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		5 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	DNA polymerase III subunit tau	[U-10% 13C]	0.6 mM
7	phosphate buffer		5 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
11	DNA polymerase III subunit tau	1.8 mM
12	phosphate buffer	5 mM
13	NaCl	100 mM
14	H2O	90 %
15	D2O	10 %

Sample #4

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
16	DNA polymerase III subunit tau	1.8 mM
17	phosphate buffer	5 mM
18	NaCl	100 mM
19	D2O	100 %

5 HNCA

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	DNA polymerase III subunit tau	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		5 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	DNA polymerase III subunit tau	[U-10% 13C]	0.6 mM
7	phosphate buffer		5 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
11	DNA polymerase III subunit tau	1.8 mM
12	phosphate buffer	5 mM
13	NaCl	100 mM
14	H2O	90 %
15	D2O	10 %

Sample #4

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
16	DNA polymerase III subunit tau	1.8 mM
17	phosphate buffer	5 mM
18	NaCl	100 mM
19	D2O	100 %

6 HN(CO)CA

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	DNA polymerase III subunit tau	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		5 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	DNA polymerase III subunit tau	[U-10% 13C]	0.6 mM
7	phosphate buffer		5 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
11	DNA polymerase III subunit tau	1.8 mM
12	phosphate buffer	5 mM
13	NaCl	100 mM
14	H2O	90 %
15	D2O	10 %

Sample #4

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
16	DNA polymerase III subunit tau	1.8 mM
17	phosphate buffer	5 mM
18	NaCl	100 mM
19	D2O	100 %

7 HNHA

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	DNA polymerase III subunit tau	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		5 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	DNA polymerase III subunit tau	[U-10% 13C]	0.6 mM
7	phosphate buffer		5 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
11	DNA polymerase III subunit tau	1.8 mM
12	phosphate buffer	5 mM
13	NaCl	100 mM
14	H2O	90 %
15	D2O	10 %

Sample #4

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
16	DNA polymerase III subunit tau	1.8 mM
17	phosphate buffer	5 mM
18	NaCl	100 mM
19	D2O	100 %

8 3D 13C-separated NOESY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	DNA polymerase III subunit tau	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		5 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	DNA polymerase III subunit tau	[U-10% 13C]	0.6 mM
7	phosphate buffer		5 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
11	DNA polymerase III subunit tau	1.8 mM
12	phosphate buffer	5 mM
13	NaCl	100 mM
14	H2O	90 %
15	D2O	10 %

Sample #4

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
16	DNA polymerase III subunit tau	1.8 mM
17	phosphate buffer	5 mM
18	NaCl	100 mM
19	D2O	100 %

9 13C-HSQC

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	DNA polymerase III subunit tau	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		5 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	DNA polymerase III subunit tau	[U-10% 13C]	0.6 mM
7	phosphate buffer		5 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
11	DNA polymerase III subunit tau	1.8 mM
12	phosphate buffer	5 mM
13	NaCl	100 mM
14	H2O	90 %
15	D2O	10 %

Sample #4

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
16	DNA polymerase III subunit tau	1.8 mM
17	phosphate buffer	5 mM
18	NaCl	100 mM
19	D2O	100 %

10 2D NOESY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	DNA polymerase III subunit tau	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		5 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	DNA polymerase III subunit tau	[U-10% 13C]	0.6 mM
7	phosphate buffer		5 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
11	DNA polymerase III subunit tau	1.8 mM
12	phosphate buffer	5 mM
13	NaCl	100 mM
14	H2O	90 %
15	D2O	10 %

Sample #4

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Concentration
16	DNA polymerase III subunit tau	1.8 mM
17	phosphate buffer	5 mM
18	NaCl	100 mM
19	D2O	100 %

nullKeywords c-terminus of polymerase III tau subunit, KH-fold

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Found 1 Document (2.095 seconds)

BMRB: 6869

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. DNA polymerase III subunit tau, : 1 : 6 : 2 entities Detail

Interaction partners 1. DNA polymerase III subunit tau, : 35 interactors Detail

Experiments performed 10 experiments Detail

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