BMRB: 6872

Solution structure of kalata B8

Authors

Daly, N.L., Clark, R.J., Plan, M.R., Craik, D.J.

Assembly

kalata B8

Entity

1. kalata B8 (polymer, Thiol state: all disulfide bound), 31 monomers, 3307.797 Da Detail

CGETCLLGTC YTTGCTCNKY RVCTKDGSVL N

Formula weight

3307.797 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS1:SG	1:CYS15:SG
2	disulfide	sing	1:CYS5:SG	1:CYS17:SG
3	disulfide	sing	1:CYS10:SG	1:CYS23:SG

Source organism

Oldenlandia affinis

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 88.9 %, Completeness (bb): 93.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	88.9 % (144 of 162)	88.9 % (144 of 162)
Backbone	93.9 % (62 of 66)	93.9 % (62 of 66)
Sidechain	85.4 % (82 of 96)	85.4 % (82 of 96)
Aromatic	50.0 % (4 of 8)	50.0 % (4 of 8)
Methyl	100.0 % (16 of 16)	100.0 % (16 of 16)

1. kalata B8

CGETCLLGTC YTTGCTCNKY RVCTKDGSVL N

Show sample condition

Sample

Pressure 1 atm, Temperature 290 K, pH 3.5

#	Name	Isotope labeling	Type	Concentration
1	kalata B8			1 mM
2	H2O			90 %
3	D2O			10 %

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Calculated from 20 models in PDB: 2B38, Strand ID: A Detail

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Release date

2005-11-09

Citation

Kalata B8, a novel antiviral circular protein, exhibits conformational flexibility in the cystine knot motif
Daly, N.L., Clark, R.J., Plan, M.R., Craik, D.J.
Biochem. J. (2006), 393, 619-626, PubMed 16207177 , DOI 10.1042/BJ20051371 , Abstract

Related entities 1. kalata B8, : 1 : 1 : 80 entities Detail

Experiments performed 4 experiments Detail