BMRBj - BMREntryMaster

Found 1 Document (2.852 seconds)

BMRB: 6874

2C52

Structural diversity in CBP/p160 complexes

Authors

Waters, L.C., Yue, B., Veverka, V., Renshaw, P.S., Bramham, J., Matsuda, S., Frenkiel, T.A., Kelly, G., Muskett, F.W., Heery, D.M., Carr, M.D.

Assembly

CBP-SID domain in complex with SRC1-AD1 domain

Entity

1. CREB-Binding Protein (polymer, Thiol state: not present), 60 monomers, 6692.699 Da Detail

MPNRSISPSA LQDLLRTLKS PSSPQQQQQV LNILKSNPQL MAAFIKQRTA KYVANQPGMQ

2. Steroid Receptor Coactivator -1 (polymer, Thiol state: not present), 68 monomers, 7432.259 Da Detail

MGPTTVEGRN DEKALLEQLV SFLSGKDETE LAELDRALGI DKLVQGGGLD VLSKLVPRGS LEHHHHHH

Total weight

14124.958 Da

Max. entity weight

7432.259 Da

Source organism

Mus musculus , Homo sapiens

Exptl. method

NMR

Refine. method

CANDID

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 58.6 %, Completeness: 46.1 %, Completeness (bb): 44.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	46.1 % (686 of 1488)	50.8 % (398 of 783)	38.7 % (220 of 568)	49.6 % (68 of 137)
Backbone	44.7 % (336 of 752)	51.4 % (132 of 257)	37.3 % (140 of 375)	53.3 % (64 of 120)
Sidechain	48.3 % (413 of 855)	50.6 % (266 of 526)	45.8 % (143 of 312)	23.5 % (4 of 17)
Aromatic	19.2 % (10 of 52)	38.5 % (10 of 26)	0.0 % (0 of 26)
Methyl	54.1 % (79 of 146)	57.5 % (42 of 73)	50.7 % (37 of 73)

1. CREB-Binding Protein

MPNRSISPSA LQDLLRTLKS PSSPQQQQQV LNILKSNPQL MAAFIKQRTA KYVANQPGMQ

2. Steroid Receptor Coactivator -1

MGPTTVEGRN DEKALLEQLV SFLSGKDETE LAELDRALGI DKLVQGGGLD VLSKLVPRGS LEHHHHHH

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	CREB-Binding Protein	[U-13C; U-15N]		1.3 ~ 1.5 mM
2	Steroid Receptor Coactivator -1	[U-13C; U-15N]		1.3 ~ 1.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	CREB-Binding Protein	[U-15N]		1.3 ~ 1.5 mM
4	Steroid Receptor Coactivator -1	[U-15N]		1.3 ~ 1.5 mM

Chem. Shift Complete2

Sequence coverage: 53.1 %, Completeness: 43.3 %, Completeness (bb): 43.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	43.3 % (1289 of 2976)	47.1 % (737 of 1566)	36.9 % (419 of 1136)	48.5 % (133 of 274)
Backbone	43.8 % (658 of 1504)	50.4 % (259 of 514)	36.3 % (272 of 750)	52.9 % (127 of 240)
Sidechain	43.9 % (751 of 1710)	45.4 % (478 of 1052)	42.8 % (267 of 624)	17.6 % (6 of 34)
Aromatic	13.5 % (14 of 104)	26.9 % (14 of 52)	0.0 % (0 of 52)
Methyl	52.4 % (153 of 292)	55.5 % (81 of 146)	49.3 % (72 of 146)

1. CREB-Binding Protein

MPNRSISPSA LQDLLRTLKS PSSPQQQQQV LNILKSNPQL MAAFIKQRTA KYVANQPGMQ

2. Steroid Receptor Coactivator -1

MGPTTVEGRN DEKALLEQLV SFLSGKDETE LAELDRALGI DKLVQGGGLD VLSKLVPRGS LEHHHHHH

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	CREB-Binding Protein	[U-13C; U-15N]		1.3 ~ 1.5 mM
2	Steroid Receptor Coactivator -1	[U-13C; U-15N]		1.3 ~ 1.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	CREB-Binding Protein	[U-15N]		1.3 ~ 1.5 mM
4	Steroid Receptor Coactivator -1	[U-15N]		1.3 ~ 1.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 37 models in PDB: 2C52, Strand ID: A, B Detail

Release date

2006-06-25

Citation

Structural diversity in p160/CREB-binding protein coactivator complexes
Waters, L.C., Yue, B., Veverka, V., Renshaw, P.S., Bramham, J., Matsuda, S., Frenkiel, T.A., Kelly, G., Muskett, F.W., Carr, M.D., Heery, D.M.
J. Biol. Chem. (2006), 281, 14787-14795, PubMed 16540468 , DOI 10.1074/jbc.M600237200 , Abstract

Related entities 1. CREB-Binding Protein, : 1 : 11 : 14 entities Detail

Related entities 2. Steroid Receptor Coactivator -1, : 1 : 10 entities Detail

Interaction partners 1. CREB-Binding Protein, : 78 : 1 interactors Detail

More details for 79 interactors identified by 55 citations

Experiments performed 9 experiments Detail

1 1H/1H TOCSY

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	CREB-Binding Protein	[U-13C; U-15N]		1.3 ~ 1.5 mM
2	Steroid Receptor Coactivator -1	[U-13C; U-15N]		1.3 ~ 1.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	CREB-Binding Protein	[U-15N]		1.3 ~ 1.5 mM
4	Steroid Receptor Coactivator -1	[U-15N]		1.3 ~ 1.5 mM

2 1H/1H NOESY

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	CREB-Binding Protein	[U-13C; U-15N]		1.3 ~ 1.5 mM
2	Steroid Receptor Coactivator -1	[U-13C; U-15N]		1.3 ~ 1.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	CREB-Binding Protein	[U-15N]		1.3 ~ 1.5 mM
4	Steroid Receptor Coactivator -1	[U-15N]		1.3 ~ 1.5 mM

3 15N/1H HSQC

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	CREB-Binding Protein	[U-13C; U-15N]		1.3 ~ 1.5 mM
2	Steroid Receptor Coactivator -1	[U-13C; U-15N]		1.3 ~ 1.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	CREB-Binding Protein	[U-15N]		1.3 ~ 1.5 mM
4	Steroid Receptor Coactivator -1	[U-15N]		1.3 ~ 1.5 mM

4 15N/1H TOCSY-HSQC

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	CREB-Binding Protein	[U-13C; U-15N]		1.3 ~ 1.5 mM
2	Steroid Receptor Coactivator -1	[U-13C; U-15N]		1.3 ~ 1.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	CREB-Binding Protein	[U-15N]		1.3 ~ 1.5 mM
4	Steroid Receptor Coactivator -1	[U-15N]		1.3 ~ 1.5 mM

5 15N/1H NOESY-HSQC

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	CREB-Binding Protein	[U-13C; U-15N]		1.3 ~ 1.5 mM
2	Steroid Receptor Coactivator -1	[U-13C; U-15N]		1.3 ~ 1.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	CREB-Binding Protein	[U-15N]		1.3 ~ 1.5 mM
4	Steroid Receptor Coactivator -1	[U-15N]		1.3 ~ 1.5 mM

6 13C/1H HCCH-TOCSY

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	CREB-Binding Protein	[U-13C; U-15N]		1.3 ~ 1.5 mM
2	Steroid Receptor Coactivator -1	[U-13C; U-15N]		1.3 ~ 1.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	CREB-Binding Protein	[U-15N]		1.3 ~ 1.5 mM
4	Steroid Receptor Coactivator -1	[U-15N]		1.3 ~ 1.5 mM

7 13C/1H HMQC-NOESY

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	CREB-Binding Protein	[U-13C; U-15N]		1.3 ~ 1.5 mM
2	Steroid Receptor Coactivator -1	[U-13C; U-15N]		1.3 ~ 1.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	CREB-Binding Protein	[U-15N]		1.3 ~ 1.5 mM
4	Steroid Receptor Coactivator -1	[U-15N]		1.3 ~ 1.5 mM

8 15N/13C/1H HNCACB

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	CREB-Binding Protein	[U-13C; U-15N]		1.3 ~ 1.5 mM
2	Steroid Receptor Coactivator -1	[U-13C; U-15N]		1.3 ~ 1.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	CREB-Binding Protein	[U-15N]		1.3 ~ 1.5 mM
4	Steroid Receptor Coactivator -1	[U-15N]		1.3 ~ 1.5 mM

9 15N/13C/1H CBCA(CO)NH

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	CREB-Binding Protein	[U-13C; U-15N]		1.3 ~ 1.5 mM
2	Steroid Receptor Coactivator -1	[U-13C; U-15N]		1.3 ~ 1.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	CREB-Binding Protein	[U-15N]		1.3 ~ 1.5 mM
4	Steroid Receptor Coactivator -1	[U-15N]		1.3 ~ 1.5 mM

null

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Found 1 Document (2.852 seconds)

BMRB: 6874

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. CREB-Binding Protein, : 1 : 11 : 14 entities Detail

Related entities 2. Steroid Receptor Coactivator -1, : 1 : 10 entities Detail

Interaction partners 1. CREB-Binding Protein, : 78 : 1 interactors Detail

Experiments performed 9 experiments Detail

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