BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	80.9 % (1010 of 1248)	85.7 % (547 of 638)	70.6 % (351 of 497)	99.1 % (112 of 113)
Backbone	82.5 % (538 of 652)	96.0 % (217 of 226)	67.7 % (218 of 322)	99.0 % (103 of 104)
Sidechain	81.9 % (570 of 696)	80.1 % (330 of 412)	84.0 % (231 of 275)	100.0 % (9 of 9)
Aromatic	53.6 % (75 of 140)	58.6 % (41 of 70)	47.1 % (32 of 68)	100.0 % (2 of 2)
Methyl	92.0 % (103 of 112)	89.3 % (50 of 56)	94.6 % (53 of 56)

1. Chagasin

GSSHKVTKAH NGATLTVAVG ELVEIQLPSN PTTGFAWYFE GGTKESPNES MFTVENKYFP PDSKLLGAGG TEHFHVTVKA AGTHAVNLTY MRPWTGPSHD SERFTVYLKA N

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
1	Chagasin	[U-13C; U-15N]	0.3 mM
2	Phosphate Buffer		20 mM
3	NaCl		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
4	Chagasin	[U-13C; U-15N]	0.7 mM
5	Phosphate Buffer		20 mM
6	NaCl		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
7	Chagasin	[U-15N]	0.5 mM
8	Phosphate Buffer		20 mM
9	NaCl		50 mM

Sample #4

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Concentration
10	Chagasin	1.2 mM
11	Phosphate Buffer	20 mM
12	NaCl	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2FO8, Strand ID: A Detail

Release date

2007-01-28

Citation

1H, 15N and 13C assignments of the cysteine protease inhibitor chagasin from Trypanosoma cruzi
Aido-Machado, R., Salmon, D., Diehl, A., Leidert, M., Schmetzer, O., Lima, A.P. DE A., Scharfstein, J., Oschkinat, H., Pires, J.R.
J. Biomol. NMR (2006), 36 Suppl 1, 30-30, PubMed 16680406 , DOI 10.1007/s10858-006-0014-x , Abstract

Related entities 1. Chagasin, : 1 : 8 : 14 entities Detail

Interaction partners 1. Chagasin, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 6 experiments Detail

1 triple-resonance experiments

Sample #1

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
1	Chagasin	[U-13C; U-15N]	0.3 mM
2	Phosphate Buffer		20 mM
3	NaCl		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
4	Chagasin	[U-13C; U-15N]	0.7 mM
5	Phosphate Buffer		20 mM
6	NaCl		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
7	Chagasin	[U-15N]	0.5 mM
8	Phosphate Buffer		20 mM
9	NaCl		50 mM

Sample #4

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Concentration
10	Chagasin	1.2 mM
11	Phosphate Buffer	20 mM
12	NaCl	50 mM

2 13C_NOESY HMQC

Sample #1

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
1	Chagasin	[U-13C; U-15N]	0.3 mM
2	Phosphate Buffer		20 mM
3	NaCl		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
4	Chagasin	[U-13C; U-15N]	0.7 mM
5	Phosphate Buffer		20 mM
6	NaCl		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
7	Chagasin	[U-15N]	0.5 mM
8	Phosphate Buffer		20 mM
9	NaCl		50 mM

Sample #4

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Concentration
10	Chagasin	1.2 mM
11	Phosphate Buffer	20 mM
12	NaCl	50 mM

3 15N NOESY HSQC

Sample #1

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
1	Chagasin	[U-13C; U-15N]	0.3 mM
2	Phosphate Buffer		20 mM
3	NaCl		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
4	Chagasin	[U-13C; U-15N]	0.7 mM
5	Phosphate Buffer		20 mM
6	NaCl		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
7	Chagasin	[U-15N]	0.5 mM
8	Phosphate Buffer		20 mM
9	NaCl		50 mM

Sample #4

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Concentration
10	Chagasin	1.2 mM
11	Phosphate Buffer	20 mM
12	NaCl	50 mM

4 2D NOESY

Sample #1

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
1	Chagasin	[U-13C; U-15N]	0.3 mM
2	Phosphate Buffer		20 mM
3	NaCl		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
4	Chagasin	[U-13C; U-15N]	0.7 mM
5	Phosphate Buffer		20 mM
6	NaCl		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
7	Chagasin	[U-15N]	0.5 mM
8	Phosphate Buffer		20 mM
9	NaCl		50 mM

Sample #4

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Concentration
10	Chagasin	1.2 mM
11	Phosphate Buffer	20 mM
12	NaCl	50 mM

5 2D TOCSY

Sample #1

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
1	Chagasin	[U-13C; U-15N]	0.3 mM
2	Phosphate Buffer		20 mM
3	NaCl		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
4	Chagasin	[U-13C; U-15N]	0.7 mM
5	Phosphate Buffer		20 mM
6	NaCl		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
7	Chagasin	[U-15N]	0.5 mM
8	Phosphate Buffer		20 mM
9	NaCl		50 mM

Sample #4

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Concentration
10	Chagasin	1.2 mM
11	Phosphate Buffer	20 mM
12	NaCl	50 mM

6 1H15N_HSQC

Sample #1

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
1	Chagasin	[U-13C; U-15N]	0.3 mM
2	Phosphate Buffer		20 mM
3	NaCl		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
4	Chagasin	[U-13C; U-15N]	0.7 mM
5	Phosphate Buffer		20 mM
6	NaCl		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Isotope labeling	Concentration
7	Chagasin	[U-15N]	0.5 mM
8	Phosphate Buffer		20 mM
9	NaCl		50 mM

Sample #4

Temperature 298 (±1) K, pH 5.6 (±0.1), Details pH 5.6

#	Name	Concentration
10	Chagasin	1.2 mM
11	Phosphate Buffer	20 mM
12	NaCl	50 mM

nullKeywords Chagasin, cysteine protease inhibitor, NMR Assignments, Trypanosoma cruzi

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BMRB: 6876

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Chagasin, : 1 : 8 : 14 entities Detail

Interaction partners 1. Chagasin, : 1 interactors Detail

Experiments performed 6 experiments Detail

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