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BMRB: 6882

2ERS

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the extracellular sushi domain of the Interleukin-15 receptor

Authors

Lorenzen, I., Dingley, A.J., Groetzinger, J.

Assembly

sushi domain of the interleukin-15 receptor

Entity

1. sushi domain of the interleukin-15 receptor (polymer, Thiol state: all disulfide bound), 66 monomers, 7462.502 Da Detail

ITCPPPMSVE HADIWVKSYS LYSRERYICN SGFKRKAGTS SLTECVLNKA TNVAHWTTPS LKCIRD

Formula weight

7462.502 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS3:SG	1:CYS45:SG
2	disulfide	sing	1:CYS29:SG	1:CYS63:SG

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.4 %, Completeness (bb): 96.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.4 % (721 of 764)	93.7 % (371 of 396)	94.7 % (285 of 301)	97.0 % (65 of 67)
Backbone	96.6 % (375 of 388)	96.9 % (126 of 130)	96.4 % (189 of 196)	96.8 % (60 of 62)
Sidechain	93.2 % (410 of 440)	92.1 % (245 of 266)	94.7 % (160 of 169)	100.0 % (5 of 5)
Aromatic	83.3 % (55 of 66)	90.9 % (30 of 33)	74.2 % (23 of 31)	100.0 % (2 of 2)
Methyl	100.0 % (68 of 68)	100.0 % (34 of 34)	100.0 % (34 of 34)

1. interleukin-15 receptor

ITCPPPMSVE HADIWVKSYS LYSRERYICN SGFKRKAGTS SLTECVLNKA TNVAHWTTPS LKCIRD

Show sample condition

Sample

Pressure 1 (±0.0) atm, Temperature 298 (±0.0) K, pH 7.4 (±0.1), Details 1mM IL-15Ra, 20 mM phosphate buffer, pH=7.4, 150 mM NaCl

#	Name	Isotope labeling	Concentration
1	interleukin-15 receptor	[U-15N; U-13C]	1 mM
2	phosphate buffer		20 mM
3	NaCl		150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 1.7 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 2ERS, Strand ID: A Detail

Release date

2006-04-23

Citation

The structure of the interleukin-15 alpha receptor and its implications for ligand binding
Lorenzen, I., Dingley, A.J., Jaques, Y., Groetzinger, J.
J. Biol. Chem. (2006), 281, 6642-6647, PubMed 16377614 , DOI 10.1074/jbc.M513118200 , Abstract

Related entities 1. sushi domain of the interleukin-15 receptor, : 1 : 5 : 20 entities Detail

Interaction partners 1. sushi domain of the interleukin-15 receptor, : 8 interactors Detail

More details for 8 interactors identified by 3 citations

Experiments performed 12 experiments Detail

1 HNCA