BMRB: 6893

Bovine Fibrinogen alpha-C Domain

Authors

Burton, R.A.

Assembly

Fibrinogen alpha chain

Entity

1. Fibrinogen alpha chain (polymer, Thiol state: all disulfide bound), 166 monomers, 18122.36 Da Detail

MGTFREEGSV SSGTKQEFHT GKLVTTKGDK ELLIDNEKVT SGHTTTTRRS CSKVITKTVT NADGRTETTK EVVKSEDGSD CGDADFDWHH TFPSRGNLDD FFHRDKDDFF TRSSHEFDGR TGLAPEFAAL GESGSSSSKT STHSKQFVSS STTVNRGGSA IESKHF

Formula weight

18122.36 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS51:SG	1:CYS81:SG

Source organism

Bos taurus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.0 %, Completeness: 46.9 %, Completeness (bb): 62.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	46.9 % (849 of 1811)	42.1 % (392 of 932)	47.0 % (333 of 708)	72.5 % (124 of 171)
Backbone	62.3 % (618 of 992)	66.5 % (230 of 346)	54.8 % (264 of 482)	75.6 % (124 of 164)
Sidechain	36.4 % (353 of 969)	27.6 % (162 of 586)	50.8 % (191 of 376)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 164)	0.0 % (0 of 82)	0.0 % (0 of 81)	0.0 % (0 of 1)
Methyl	55.4 % (72 of 130)	60.0 % (39 of 65)	50.8 % (33 of 65)

1. Fibrinogen alpha chain

MGTFREEGSV SSGTKQEFHT GKLVTTKGDK ELLIDNEKVT SGHTTTTRRS CSKVITKTVT NADGRTETTK EVVKSEDGSD CGDADFDWHH TFPSRGNLDD FFHRDKDDFF TRSSHEFDGR TGLAPEFAAL GESGSSSSKT STHSKQFVSS STTVNRGGSA IESKHF

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Fibrinogen alpha chain	[U-15N; U-13C]		0.2 mM
2	phosphate buffer			10 mM
3	NaCl			150 mM
4	H20			90 %
5	D20			10 %

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LACS Plot; CA

Referencing offset: 0.09 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	na	0.0 ppm	null	null	0.0
1H	TSP	na	0.0 ppm	null	null	0.0
15N	TSP	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: 0.09 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	na	0.0 ppm	null	null	0.0
1H	TSP	na	0.0 ppm	null	null	0.0
15N	TSP	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	na	0.0 ppm	null	null	0.0
1H	TSP	na	0.0 ppm	null	null	0.0
15N	TSP	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 2BAF, Strand ID: A Detail

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Release date

2005-11-28

Citation

Identification of an ordered compact structure within the recombinant bovine fibrinogen alphaC-domain fragment by NMR
Burton, R.A., Tsurupa, G., Medved, L., Tjandra, N.
Biochemistry (2006), 45, 2257-2266, PubMed 16475814 , DOI 10.1021/bi052380c , Abstract

Related entities 1. Fibrinogen alpha chain, : 1 : 1 : 9 entities Detail

Experiments performed 1 experiments Detail