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BMRB: 6895

2ERR

NMR Structure of the RNA Binding Domain of Human Fox-1 in Complex with UGCAUGU

Authors

Allain, F.H.-T., Auweter, S.D.

Assembly

PROTEIN/RNA Complex

Entity

1. Ataxin-2-binding protein 1 (polymer, Thiol state: not present), 109 monomers, 12374.77 Da Detail

MGSSHHHHHH SSGLVPRGSH MNTENKSQPK RLHVSNIPFR FRDPDLRQMF GQFGKILDVE IIFNERGSKG FGFVTFENSA DADRAREKLH GTVVEGRKIE VNNATARVM

2. UGCAUGU (polymer, Thiol state: not present), 7 monomers, 2261.311 Da Detail

UGCAUGU

Total weight

14636.08 Da

Max. entity weight

12374.77 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 71.6 %, Completeness: 55.1 %, Completeness (bb): 55.3 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	55.1 % (763 of 1384)	60.0 % (437 of 728)	44.8 % (240 of 536)	71.7 % (86 of 120)
Backbone	55.3 % (400 of 723)	61.7 % (164 of 266)	44.6 % (157 of 352)	75.2 % (79 of 105)
Sidechain	57.1 % (434 of 760)	59.1 % (273 of 462)	54.4 % (154 of 283)	46.7 % (7 of 15)
Aromatic	18.0 % (27 of 150)	36.0 % (27 of 75)	0.0 % (0 of 70)	0.0 % (0 of 5)
Methyl	81.1 % (73 of 90)	82.2 % (37 of 45)	80.0 % (36 of 45)

1. Ataxin-2-binding protein 1

MGSSHHHHHH SSGLVPRGSH MNTENKSQPK RLHVSNIPFR FRDPDLRQMF GQFGKILDVE IIFNERGSKG FGFVTFENSA DADRAREKLH GTVVEGRKIE VNNATARVM

2. UGCAUGU

UGCAUGU

Show sample condition

Sample

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ataxin-2-binding protein 1	[U-15N]	1 mM
2	UGCAUGU		1 mM
3	sodium phoshphate		20 mM
4	NaCl		30 mM
5	H2O		90 %
6	D2O		10 %

Chem. Shift Complete2

Sequence coverage: 17.2 %, Completeness: 33.3 %, Completeness (bb): 35.1 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	33.3 % (922 of 2768)	37.4 % (545 of 1456)	26.0 % (279 of 1072)	40.8 % (98 of 240)
Backbone	35.1 % (508 of 1446)	43.2 % (230 of 532)	26.6 % (187 of 704)	43.3 % (91 of 210)
Sidechain	32.5 % (494 of 1520)	34.1 % (315 of 924)	30.4 % (172 of 566)	23.3 % (7 of 30)
Aromatic	13.0 % (39 of 300)	26.0 % (39 of 150)	0.0 % (0 of 140)	0.0 % (0 of 10)
Methyl	47.2 % (85 of 180)	48.9 % (44 of 90)	45.6 % (41 of 90)

1. Ataxin-2-binding protein 1

MGSSHHHHHH SSGLVPRGSH MNTENKSQPK RLHVSNIPFR FRDPDLRQMF GQFGKILDVE IIFNERGSKG FGFVTFENSA DADRAREKLH GTVVEGRKIE VNNATARVM

2. UGCAUGU

UGCAUGU

Show sample condition

Sample

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ataxin-2-binding protein 1	[U-15N]	1 mM
2	UGCAUGU		1 mM
3	sodium phoshphate		20 mM
4	NaCl		30 mM
5	H2O		90 %
6	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.78 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.78 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 2ERR, Strand ID: A Detail

Release date

2006-02-22

Citation

Molecular basis of RNA recognition by the human alternative splicing factor Fox-1
Auweter, S.D., Fasan, R., Reymond, L., Underwood, J.G., Black, D.L., Pitsch, S., Allain, F.H.-T.
EMBO J. (2006), 25, 163-173, PubMed 16362037 , DOI 10.1038/sj.emboj.7600918 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q9NWB1 released on 2000-12-01
Title RFOX1_HUMAN Entity RNA binding protein fox-1 homolog 1

Related entities 1. Ataxin-2-binding protein 1, : 1 : 8 : 74 entities Detail

Experiments performed 11 experiments Detail

1 2D NOESY

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ataxin-2-binding protein 1	[U-15N]	1 mM
2	UGCAUGU		1 mM
3	sodium phoshphate		20 mM
4	NaCl		30 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
8	UGCAUGU		1 mM
9	sodium phoshphate		20 mM
10	NaCl		30 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Ataxin-2-binding protein 1	[U-15N]	1 mM
14	UGCAUGU		1 mM
15	sodium phoshphate		20 mM
16	NaCl		30 mM
17	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
19	UGCAUGU		1 mM
20	sodium phoshphate		20 mM
21	NaCl		30 mM
22	D2O		100 %

2 3D 13C-separated NOESY

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ataxin-2-binding protein 1	[U-15N]	1 mM
2	UGCAUGU		1 mM
3	sodium phoshphate		20 mM
4	NaCl		30 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
8	UGCAUGU		1 mM
9	sodium phoshphate		20 mM
10	NaCl		30 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Ataxin-2-binding protein 1	[U-15N]	1 mM
14	UGCAUGU		1 mM
15	sodium phoshphate		20 mM
16	NaCl		30 mM
17	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
19	UGCAUGU		1 mM
20	sodium phoshphate		20 mM
21	NaCl		30 mM
22	D2O		100 %

3 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ataxin-2-binding protein 1	[U-15N]	1 mM
2	UGCAUGU		1 mM
3	sodium phoshphate		20 mM
4	NaCl		30 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
8	UGCAUGU		1 mM
9	sodium phoshphate		20 mM
10	NaCl		30 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Ataxin-2-binding protein 1	[U-15N]	1 mM
14	UGCAUGU		1 mM
15	sodium phoshphate		20 mM
16	NaCl		30 mM
17	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
19	UGCAUGU		1 mM
20	sodium phoshphate		20 mM
21	NaCl		30 mM
22	D2O		100 %

4 HNCA

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ataxin-2-binding protein 1	[U-15N]	1 mM
2	UGCAUGU		1 mM
3	sodium phoshphate		20 mM
4	NaCl		30 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
8	UGCAUGU		1 mM
9	sodium phoshphate		20 mM
10	NaCl		30 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Ataxin-2-binding protein 1	[U-15N]	1 mM
14	UGCAUGU		1 mM
15	sodium phoshphate		20 mM
16	NaCl		30 mM
17	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
19	UGCAUGU		1 mM
20	sodium phoshphate		20 mM
21	NaCl		30 mM
22	D2O		100 %

5 CBCACONH

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ataxin-2-binding protein 1	[U-15N]	1 mM
2	UGCAUGU		1 mM
3	sodium phoshphate		20 mM
4	NaCl		30 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
8	UGCAUGU		1 mM
9	sodium phoshphate		20 mM
10	NaCl		30 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Ataxin-2-binding protein 1	[U-15N]	1 mM
14	UGCAUGU		1 mM
15	sodium phoshphate		20 mM
16	NaCl		30 mM
17	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
19	UGCAUGU		1 mM
20	sodium phoshphate		20 mM
21	NaCl		30 mM
22	D2O		100 %

6 HNCOCA

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ataxin-2-binding protein 1	[U-15N]	1 mM
2	UGCAUGU		1 mM
3	sodium phoshphate		20 mM
4	NaCl		30 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
8	UGCAUGU		1 mM
9	sodium phoshphate		20 mM
10	NaCl		30 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Ataxin-2-binding protein 1	[U-15N]	1 mM
14	UGCAUGU		1 mM
15	sodium phoshphate		20 mM
16	NaCl		30 mM
17	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
19	UGCAUGU		1 mM
20	sodium phoshphate		20 mM
21	NaCl		30 mM
22	D2O		100 %

7 HCCH-TOCSY

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ataxin-2-binding protein 1	[U-15N]	1 mM
2	UGCAUGU		1 mM
3	sodium phoshphate		20 mM
4	NaCl		30 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
8	UGCAUGU		1 mM
9	sodium phoshphate		20 mM
10	NaCl		30 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Ataxin-2-binding protein 1	[U-15N]	1 mM
14	UGCAUGU		1 mM
15	sodium phoshphate		20 mM
16	NaCl		30 mM
17	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
19	UGCAUGU		1 mM
20	sodium phoshphate		20 mM
21	NaCl		30 mM
22	D2O		100 %

8 2D F1

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ataxin-2-binding protein 1	[U-15N]	1 mM
2	UGCAUGU		1 mM
3	sodium phoshphate		20 mM
4	NaCl		30 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
8	UGCAUGU		1 mM
9	sodium phoshphate		20 mM
10	NaCl		30 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Ataxin-2-binding protein 1	[U-15N]	1 mM
14	UGCAUGU		1 mM
15	sodium phoshphate		20 mM
16	NaCl		30 mM
17	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
19	UGCAUGU		1 mM
20	sodium phoshphate		20 mM
21	NaCl		30 mM
22	D2O		100 %

9 F2-edited NOESY

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ataxin-2-binding protein 1	[U-15N]	1 mM
2	UGCAUGU		1 mM
3	sodium phoshphate		20 mM
4	NaCl		30 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
8	UGCAUGU		1 mM
9	sodium phoshphate		20 mM
10	NaCl		30 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Ataxin-2-binding protein 1	[U-15N]	1 mM
14	UGCAUGU		1 mM
15	sodium phoshphate		20 mM
16	NaCl		30 mM
17	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
19	UGCAUGU		1 mM
20	sodium phoshphate		20 mM
21	NaCl		30 mM
22	D2O		100 %

10 2D F1-edited

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ataxin-2-binding protein 1	[U-15N]	1 mM
2	UGCAUGU		1 mM
3	sodium phoshphate		20 mM
4	NaCl		30 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
8	UGCAUGU		1 mM
9	sodium phoshphate		20 mM
10	NaCl		30 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Ataxin-2-binding protein 1	[U-15N]	1 mM
14	UGCAUGU		1 mM
15	sodium phoshphate		20 mM
16	NaCl		30 mM
17	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
19	UGCAUGU		1 mM
20	sodium phoshphate		20 mM
21	NaCl		30 mM
22	D2O		100 %

11 F2-fitered NOESY

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ataxin-2-binding protein 1	[U-15N]	1 mM
2	UGCAUGU		1 mM
3	sodium phoshphate		20 mM
4	NaCl		30 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
8	UGCAUGU		1 mM
9	sodium phoshphate		20 mM
10	NaCl		30 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Ataxin-2-binding protein 1	[U-15N]	1 mM
14	UGCAUGU		1 mM
15	sodium phoshphate		20 mM
16	NaCl		30 mM
17	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Ataxin-2-binding protein 1	[U-13C; U-15N]	1 mM
19	UGCAUGU		1 mM
20	sodium phoshphate		20 mM
21	NaCl		30 mM
22	D2O		100 %

nullKeywords PROTEIN-RNA COMPLEX

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Found 1 Document (1.909 seconds)

BMRB: 6895

Sample

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Ataxin-2-binding protein 1, : 1 : 8 : 74 entities Detail

Experiments performed 11 experiments Detail

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