BMRB: 6898

NMR structure of the peptide linked to the genome (VPg) of poliovirus

Authors

Schein, C.H., Oezguen, N.

Assembly

Genome linked protein VPg

Entity

1. Genome linked protein VPg (polymer, Thiol state: not present), 22 monomers, 2353.715 Da Detail

GAYTGLPNKK PNVPTIRTAK VQ

Formula weight

2353.715 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

automatic NOE assignment in combination with distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 83.8 %, Completeness (bb): 93.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	83.8 % (114 of 136)	83.8 % (114 of 136)
Backbone	93.0 % (40 of 43)	93.0 % (40 of 43)
Sidechain	79.6 % (74 of 93)	79.6 % (74 of 93)
Aromatic	100.0 % (4 of 4)	100.0 % (4 of 4)
Methyl	92.3 % (12 of 13)	92.3 % (12 of 13)

1. Genome linked protein VPg

GAYTGLPNKK PNVPTIRTAK VQ

Show sample condition

Sample #1

Pressure 1 atm, Temperature 283 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	Genome linked protein VPg			2.78 mM
2	sodium phosphate buffer			0.01 M
3	deuterated Trimethylamine oxide			1 M
4	D2O			10 %
5	H2O			90 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
6	Genome linked protein VPg			3.7 mM
7	sodium phosphate buffer			0.01 M
8	D2O			10 %
9	H2O			90 %

Hide sample codition

Chem. Shift Complete2

Sequence coverage: 95.5 %, Completeness: 81.3 %, Completeness (bb): 88.4 % Detail

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• BMRB/XML (partial)
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Polymer type: polypeptide(L)

	Total	1H
All	81.3 % (221 of 272)	81.3 % (221 of 272)
Backbone	88.4 % (76 of 86)	88.4 % (76 of 86)
Sidechain	78.0 % (145 of 186)	78.0 % (145 of 186)
Aromatic	100.0 % (8 of 8)	100.0 % (8 of 8)
Methyl	92.3 % (24 of 26)	92.3 % (24 of 26)

1. Genome linked protein VPg

GAYTGLPNKK PNVPTIRTAK VQ

Show sample condition

Sample

Pressure 1 atm, Temperature 283 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
6	Genome linked protein VPg			3.7 mM
7	sodium phosphate buffer			0.01 M
8	D2O			10 %
9	H2O			90 %

Hide sample codition

Protein Blocks Logo

Calculated from 10 models in PDB: 2BBP, Strand ID: A Detail

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Release date

2007-02-05

Citation

NMR structure of the viral peptide linked to the genome (VPg) of poliovirus
Schein, C.H., Oezguen, N.N., Volk, D.E., Garimella, R., Paul, A., Braun, W.
Peptides (2006), 27, 1676-1684, PubMed 16540201 , DOI 10.1016/j.peptides.2006.01.018 , Abstract

Related entities 1. Genome linked protein VPg, : 1 : 3 : 4 : 1 : 27 entities Detail

Experiments performed 3 experiments Detail