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Found 1 Document (1.65 seconds)

BMRB: 6912

2I9H

1H, 13C, and 15N Resonance Assignments for the reduced form of Thioredoxin 1 from Sacharomyces cerevisae

Authors

Pinheiro, A., Amorim, G., Luis Eduardo, N., Ana Paula, V., Fabio, A.

Assembly

yeast thioredoxin 1

Entity

1. yeast thioredoxin 1 (polymer, Thiol state: all free), 103 monomers, 11234.85 Da Detail

MVTQFKTASE FDSAIAQDKL VVVDFYATWC GPCKMIAPMI EKFSEQYPQA DFYKLDVDEL GDVAQKNEVS AMPTLLLFKN GKEVAKVVGA NPAAIKQAIA ANA

Formula weight

11234.85 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

torsion angle simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.8 %, Completeness (bb): 96.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.8 % (1106 of 1192)	93.3 % (573 of 614)	91.3 % (428 of 469)	96.3 % (105 of 109)
Backbone	96.2 % (585 of 608)	97.6 % (200 of 205)	95.4 % (291 of 305)	95.9 % (94 of 98)
Sidechain	90.6 % (619 of 683)	91.2 % (373 of 409)	89.4 % (235 of 263)	100.0 % (11 of 11)
Aromatic	91.7 % (88 of 96)	95.8 % (46 of 48)	87.2 % (41 of 47)	100.0 % (1 of 1)
Methyl	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)

1. Trx1

MVTQFKTASE FDSAIAQDKL VVVDFYATWC GPCKMIAPMI EKFSEQYPQA DFYKLDVDEL GDVAQKNEVS AMPTLLLFKN GKEVAKVVGA NPAAIKQAIA ANA

Show sample condition

Sample

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	thioredoxin 2	[U-13C; U-15N]	1 mM
2	2-mercaptoethanol		3 mM
3	H2O		90 %
4	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.23 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.23 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 3.95 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2I9H, Strand ID: A Detail

Release date

2006-04-13

Citation

1H, 13C and 15N resonance assignments for the reduced forms of thioredoxin 1 and 2 from S. cerevisiae
Pinheiro, A.S., Amorim, G.C., Netto, L.S., Valente, A., Almeida, F.C. L.
J. Biomol. NMR (2006), 36 Suppl 1, 35-35, PubMed 16609834 , DOI 10.1007/s10858-006-0025-7 , Abstract

Related entities 1. yeast thioredoxin 1, : 1 : 3 : 3 : 264 entities Detail

Interaction partners 1. yeast thioredoxin 1, : 15 interactors Detail

More details for 15 interactors identified by 5 citations

Experiments performed 9 experiments Detail

1 HNCA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	thioredoxin 2	[U-13C; U-15N]	1 mM
2	2-mercaptoethanol		3 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	thioredoxin 2	[U-15N]	1.2 mM
6	DTT		3 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Concentration
9	thioredoxin 2	1.2 mM
10	DTT	3 mM
11	D2O	99 %

2 HN(CO)CA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	thioredoxin 2	[U-13C; U-15N]	1 mM
2	2-mercaptoethanol		3 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	thioredoxin 2	[U-15N]	1.2 mM
6	DTT		3 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Concentration
9	thioredoxin 2	1.2 mM
10	DTT	3 mM
11	D2O	99 %

3 HNCACB

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	thioredoxin 2	[U-13C; U-15N]	1 mM
2	2-mercaptoethanol		3 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	thioredoxin 2	[U-15N]	1.2 mM
6	DTT		3 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Concentration
9	thioredoxin 2	1.2 mM
10	DTT	3 mM
11	D2O	99 %

4 CBCA(CO)NH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	thioredoxin 2	[U-13C; U-15N]	1 mM
2	2-mercaptoethanol		3 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	thioredoxin 2	[U-15N]	1.2 mM
6	DTT		3 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Concentration
9	thioredoxin 2	1.2 mM
10	DTT	3 mM
11	D2O	99 %

5 HNCO

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	thioredoxin 2	[U-13C; U-15N]	1 mM
2	2-mercaptoethanol		3 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	thioredoxin 2	[U-15N]	1.2 mM
6	DTT		3 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Concentration
9	thioredoxin 2	1.2 mM
10	DTT	3 mM
11	D2O	99 %

6 H(CCO)NH-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	thioredoxin 2	[U-13C; U-15N]	1 mM
2	2-mercaptoethanol		3 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	thioredoxin 2	[U-15N]	1.2 mM
6	DTT		3 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Concentration
9	thioredoxin 2	1.2 mM
10	DTT	3 mM
11	D2O	99 %

7 (H)CC(CO)NH-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	thioredoxin 2	[U-13C; U-15N]	1 mM
2	2-mercaptoethanol		3 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	thioredoxin 2	[U-15N]	1.2 mM
6	DTT		3 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Concentration
9	thioredoxin 2	1.2 mM
10	DTT	3 mM
11	D2O	99 %

8 HCCH-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	thioredoxin 2	[U-13C; U-15N]	1 mM
2	2-mercaptoethanol		3 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	thioredoxin 2	[U-15N]	1.2 mM
6	DTT		3 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Concentration
9	thioredoxin 2	1.2 mM
10	DTT	3 mM
11	D2O	99 %

9 1H-1H_TOCSY_2D

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	thioredoxin 2	[U-13C; U-15N]	1 mM
2	2-mercaptoethanol		3 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	thioredoxin 2	[U-15N]	1.2 mM
6	DTT		3 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Temperature 308 (±0.2) K, pH 7.0 (±0.2)

#	Name	Concentration
9	thioredoxin 2	1.2 mM
10	DTT	3 mM
11	D2O	99 %

null

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Found 1 Document (1.65 seconds)

BMRB: 6912

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. yeast thioredoxin 1, : 1 : 3 : 3 : 264 entities Detail

Interaction partners 1. yeast thioredoxin 1, : 15 interactors Detail

Experiments performed 9 experiments Detail

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