BMRB: 6916

NMR structure of the C-terminal domain (dimer) of HPV45 oncoprotein E7

Authors

Ohlenschlager, O., Gorlach, M.

Assembly

Protein E7

Entity

1. Protein E7 (polymer, Thiol state: free and other bound), 56 monomers, 6298.232 × 2 Da Detail

GSHMAEPQRH KILCVCCKCD GRIELTVESS AEDLRTLQQL FLSTLSFVCP WCATNQ

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

12727.282 Da

Max. entity weight

6298.232 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS16:SG	2:ZN1:ZN
2	na	sing	1:CYS19:SG	2:ZN1:ZN
3	na	sing	1:CYS49:SG	2:ZN1:ZN
4	na	sing	1:CYS52:SG	2:ZN1:ZN
5	na	sing	1:CYS16:SG	2:ZN1:ZN
6	na	sing	1:CYS19:SG	2:ZN1:ZN
7	na	sing	1:CYS49:SG	2:ZN1:ZN
8	na	sing	1:CYS52:SG	2:ZN1:ZN

Source organism

Human papillomavirus

Exptl. method

NMR

Refine. method

distance geometry/simulated annealing by CYANA, energy minimisation by OPAL

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 95.1 %, Completeness (bb): 94.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.1 % (608 of 639)	95.5 % (316 of 331)	95.2 % (236 of 248)	93.3 % (56 of 60)
Backbone	94.6 % (314 of 332)	94.6 % (106 of 112)	95.2 % (158 of 166)	92.6 % (50 of 54)
Sidechain	96.1 % (347 of 361)	95.9 % (210 of 219)	96.3 % (131 of 136)	100.0 % (6 of 6)
Aromatic	80.0 % (32 of 40)	80.0 % (16 of 20)	78.9 % (15 of 19)	100.0 % (1 of 1)
Methyl	100.0 % (62 of 62)	100.0 % (31 of 31)	100.0 % (31 of 31)

1. Protein E7

GSHMAEPQRH KILCVCCKCD GRIELTVESS AEDLRTLQQL FLSTLSFVCP WCATNQ

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	Protein E7	[U-15N]		1 mM
2	d-TRIS			20 mM
3	NaCl			100 mM
4	H2O			90 %
5	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.4 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DIOXANE	methylene carbons	67.8 ppm	external	direct	1.0
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	NH4Cl	nitrogen	24.93 ppm	external	direct	1.0

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LACS Plot; CB

Referencing offset: -0.4 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DIOXANE	methylene carbons	67.8 ppm	external	direct	1.0
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	NH4Cl	nitrogen	24.93 ppm	external	direct	1.0

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LACS Plot; HA

Referencing offset: -0.15 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DIOXANE	methylene carbons	67.8 ppm	external	direct	1.0
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	NH4Cl	nitrogen	24.93 ppm	external	direct	1.0

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LACS Plot; CO

Referencing offset: 1.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DIOXANE	methylene carbons	67.8 ppm	external	direct	1.0
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	NH4Cl	nitrogen	24.93 ppm	external	direct	1.0

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Protein Blocks Logo

Calculated from 15 models in PDB: 2EWL, Strand ID: A Detail

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Release date

2006-05-01

Citation

Solution structure of the partially folded high-risk human papilloma virus 45 oncoprotein E7
Ohlenschlager, O., Seiboth, T., Zengerling, H., Briese, L., Marchanka, A., Ramachandran, R., Baum, M., Korbas, M., Meyer-Klaucke, W., Durst, M., Gorlach, M.
Oncogene (2006), 25, 5953-5959, PubMed 16636661 , DOI 10.1038/sj.onc.1209584 , Abstract

Related entities 1. Protein E7, : 1 : 1 : 1 : 51 entities Detail

Experiments performed 3 experiments Detail