BMRBj - BMREntryMaster

Found 1 Document (2.864 seconds)

BMRB: 6919

2CH0

Letter to the Editor: 1H, 13C and 15N resonance assignments of the region 655-775 of the human MAN1

Authors

Caputo, S., Feng, L., Gilquin, B., Worman, H.J., Zinn-Justin, S., Couprie, J.

Assembly

MAN1 655-775 domain

Entity

1. MAN1 655-775 domain (polymer, Thiol state: all free), 133 monomers, 15891.97 Da Detail

GSPEFRWTKE EEETRQMYDM VVKIIDVLRS HNEACQENKD LQPYMPIPHV RDSLIQPHDR KKMKKVWDRA VDFLAANESR VRTETRRIGG ADFLVWRWIQ PSASCDKILV IPSKVWQGQA FHLDRRLERP HRD

Formula weight

15891.97 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY, SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 82.7 %, Completeness: 71.7 %, Completeness (bb): 75.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	71.7 % (1171 of 1634)	71.9 % (623 of 866)	70.5 % (443 of 628)	75.0 % (105 of 140)
Backbone	75.6 % (591 of 782)	76.0 % (199 of 262)	75.2 % (297 of 395)	76.0 % (95 of 125)
Sidechain	68.9 % (676 of 981)	70.2 % (424 of 604)	66.9 % (242 of 362)	66.7 % (10 of 15)
Aromatic	50.7 % (69 of 136)	52.9 % (36 of 68)	46.0 % (29 of 63)	80.0 % (4 of 5)
Methyl	88.1 % (111 of 126)	93.7 % (59 of 63)	82.5 % (52 of 63)

1. MAN1 655-775

GSPEFRWTKE EEETRQMYDM VVKIIDVLRS HNEACQENKD LQPYMPIPHV RDSLIQPHDR KKMKKVWDRA VDFLAANESR VRTETRRIGG ADFLVWRWIQ PSASCDKILV IPSKVWQGQA FHLDRRLERP HRD

Show sample condition

Sample

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MAN1655-775	[U-99% 15N; U-10% 2H]	protein	0.8 mM
2	Phosphate		buffer	50 mM
3	NaCl		salt	150 mM
4	EDTA		chelating agent	1 mM
5	TCEP		additive	2 mM
6	TSP		internal reference	2 mM
7	NaN3		antimicrobial agent	0.01 %
8	D2O		solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2CH0, Strand ID: A Detail

Release date

2005-12-15

Citation

NMR assignment of region 655-775 of human MAN1
Caputo, S., Lin, F., Gilquin, B., Worman, H.J., Zinn-Justin, S., Couprie, J.
J. Biomol. NMR (2006), 36 Suppl 1, 2-2, PubMed 16601860 , DOI 10.1007/s10858-005-2628-9 , Abstract

Related entities 1. MAN1 655-775 domain, : 1 : 6 entities Detail

Interaction partners 1. MAN1 655-775 domain, : 20 interactors Detail

More details for 20 interactors identified by 10 citations

Experiments performed 13 experiments Detail

1 1H15N_HSQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (900 MHz)

Sample #1

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MAN1655-775	[U-99% 15N; U-10% 2H]	protein	0.8 mM
2	Phosphate		buffer	50 mM
3	NaCl		salt	150 mM
4	EDTA		chelating agent	1 mM
5	TCEP		additive	2 mM
6	TSP		internal reference	2 mM
7	NaN3		antimicrobial agent	0.01 %
8	D2O		solvent	10 %

Sample #2

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	MAN1655-775	[U-99% 15N; U-99% 13C; U10% 2H]	protein	0.8 mM
10	Phosphate		buffer	50 mM
11	NaCl		salt	150 mM
12	EDTA		chelating agent	1 mM
13	TCEP		additive	2 mM
14	TSP		internal reference	2 mM
15	NaN3		antimicrobial agent	0.01 %
16	D2O		solvent	10 %

Sample #3

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
17	MAN1655-775	[U-99% 15N; U-99% 13C; U-100% 2H]	protein	0.8 mM
18	Phosphate		buffer	50 mM
19	NaCl		salt	150 mM
20	EDTA		chelating agent	1 mM
21	TCEP		additive	2 mM
22	TSP		internal reference	2 mM
23	NaN3		antimicrobial agent	0.01 %
24	D2O		solvent	10 %

2 HNCO

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (900 MHz)

Sample #1

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MAN1655-775	[U-99% 15N; U-10% 2H]	protein	0.8 mM
2	Phosphate		buffer	50 mM
3	NaCl		salt	150 mM
4	EDTA		chelating agent	1 mM
5	TCEP		additive	2 mM
6	TSP		internal reference	2 mM
7	NaN3		antimicrobial agent	0.01 %
8	D2O		solvent	10 %

Sample #2

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	MAN1655-775	[U-99% 15N; U-99% 13C; U10% 2H]	protein	0.8 mM
10	Phosphate		buffer	50 mM
11	NaCl		salt	150 mM
12	EDTA		chelating agent	1 mM
13	TCEP		additive	2 mM
14	TSP		internal reference	2 mM
15	NaN3		antimicrobial agent	0.01 %
16	D2O		solvent	10 %

Sample #3

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
17	MAN1655-775	[U-99% 15N; U-99% 13C; U-100% 2H]	protein	0.8 mM
18	Phosphate		buffer	50 mM
19	NaCl		salt	150 mM
20	EDTA		chelating agent	1 mM
21	TCEP		additive	2 mM
22	TSP		internal reference	2 mM
23	NaN3		antimicrobial agent	0.01 %
24	D2O		solvent	10 %

3 HNCA

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (900 MHz)

Sample #1

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MAN1655-775	[U-99% 15N; U-10% 2H]	protein	0.8 mM
2	Phosphate		buffer	50 mM
3	NaCl		salt	150 mM
4	EDTA		chelating agent	1 mM
5	TCEP		additive	2 mM
6	TSP		internal reference	2 mM
7	NaN3		antimicrobial agent	0.01 %
8	D2O		solvent	10 %

Sample #2

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	MAN1655-775	[U-99% 15N; U-99% 13C; U10% 2H]	protein	0.8 mM
10	Phosphate		buffer	50 mM
11	NaCl		salt	150 mM
12	EDTA		chelating agent	1 mM
13	TCEP		additive	2 mM
14	TSP		internal reference	2 mM
15	NaN3		antimicrobial agent	0.01 %
16	D2O		solvent	10 %

Sample #3

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
17	MAN1655-775	[U-99% 15N; U-99% 13C; U-100% 2H]	protein	0.8 mM
18	Phosphate		buffer	50 mM
19	NaCl		salt	150 mM
20	EDTA		chelating agent	1 mM
21	TCEP		additive	2 mM
22	TSP		internal reference	2 mM
23	NaN3		antimicrobial agent	0.01 %
24	D2O		solvent	10 %

4 HNCACB

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (900 MHz)

Sample #1

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MAN1655-775	[U-99% 15N; U-10% 2H]	protein	0.8 mM
2	Phosphate		buffer	50 mM
3	NaCl		salt	150 mM
4	EDTA		chelating agent	1 mM
5	TCEP		additive	2 mM
6	TSP		internal reference	2 mM
7	NaN3		antimicrobial agent	0.01 %
8	D2O		solvent	10 %

Sample #2

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	MAN1655-775	[U-99% 15N; U-99% 13C; U10% 2H]	protein	0.8 mM
10	Phosphate		buffer	50 mM
11	NaCl		salt	150 mM
12	EDTA		chelating agent	1 mM
13	TCEP		additive	2 mM
14	TSP		internal reference	2 mM
15	NaN3		antimicrobial agent	0.01 %
16	D2O		solvent	10 %

Sample #3

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
17	MAN1655-775	[U-99% 15N; U-99% 13C; U-100% 2H]	protein	0.8 mM
18	Phosphate		buffer	50 mM
19	NaCl		salt	150 mM
20	EDTA		chelating agent	1 mM
21	TCEP		additive	2 mM
22	TSP		internal reference	2 mM
23	NaN3		antimicrobial agent	0.01 %
24	D2O		solvent	10 %

5 CBCA(CO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (900 MHz)

Sample #1

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MAN1655-775	[U-99% 15N; U-10% 2H]	protein	0.8 mM
2	Phosphate		buffer	50 mM
3	NaCl		salt	150 mM
4	EDTA		chelating agent	1 mM
5	TCEP		additive	2 mM
6	TSP		internal reference	2 mM
7	NaN3		antimicrobial agent	0.01 %
8	D2O		solvent	10 %

Sample #2

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	MAN1655-775	[U-99% 15N; U-99% 13C; U10% 2H]	protein	0.8 mM
10	Phosphate		buffer	50 mM
11	NaCl		salt	150 mM
12	EDTA		chelating agent	1 mM
13	TCEP		additive	2 mM
14	TSP		internal reference	2 mM
15	NaN3		antimicrobial agent	0.01 %
16	D2O		solvent	10 %

Sample #3

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
17	MAN1655-775	[U-99% 15N; U-99% 13C; U-100% 2H]	protein	0.8 mM
18	Phosphate		buffer	50 mM
19	NaCl		salt	150 mM
20	EDTA		chelating agent	1 mM
21	TCEP		additive	2 mM
22	TSP		internal reference	2 mM
23	NaN3		antimicrobial agent	0.01 %
24	D2O		solvent	10 %

6 (HCA)CO(CA)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (900 MHz)

Sample #1

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MAN1655-775	[U-99% 15N; U-10% 2H]	protein	0.8 mM
2	Phosphate		buffer	50 mM
3	NaCl		salt	150 mM
4	EDTA		chelating agent	1 mM
5	TCEP		additive	2 mM
6	TSP		internal reference	2 mM
7	NaN3		antimicrobial agent	0.01 %
8	D2O		solvent	10 %

Sample #2

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	MAN1655-775	[U-99% 15N; U-99% 13C; U10% 2H]	protein	0.8 mM
10	Phosphate		buffer	50 mM
11	NaCl		salt	150 mM
12	EDTA		chelating agent	1 mM
13	TCEP		additive	2 mM
14	TSP		internal reference	2 mM
15	NaN3		antimicrobial agent	0.01 %
16	D2O		solvent	10 %

Sample #3

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
17	MAN1655-775	[U-99% 15N; U-99% 13C; U-100% 2H]	protein	0.8 mM
18	Phosphate		buffer	50 mM
19	NaCl		salt	150 mM
20	EDTA		chelating agent	1 mM
21	TCEP		additive	2 mM
22	TSP		internal reference	2 mM
23	NaN3		antimicrobial agent	0.01 %
24	D2O		solvent	10 %

7 CBCACOHA

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (900 MHz)

Sample #1

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MAN1655-775	[U-99% 15N; U-10% 2H]	protein	0.8 mM
2	Phosphate		buffer	50 mM
3	NaCl		salt	150 mM
4	EDTA		chelating agent	1 mM
5	TCEP		additive	2 mM
6	TSP		internal reference	2 mM
7	NaN3		antimicrobial agent	0.01 %
8	D2O		solvent	10 %

Sample #2

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	MAN1655-775	[U-99% 15N; U-99% 13C; U10% 2H]	protein	0.8 mM
10	Phosphate		buffer	50 mM
11	NaCl		salt	150 mM
12	EDTA		chelating agent	1 mM
13	TCEP		additive	2 mM
14	TSP		internal reference	2 mM
15	NaN3		antimicrobial agent	0.01 %
16	D2O		solvent	10 %

Sample #3

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
17	MAN1655-775	[U-99% 15N; U-99% 13C; U-100% 2H]	protein	0.8 mM
18	Phosphate		buffer	50 mM
19	NaCl		salt	150 mM
20	EDTA		chelating agent	1 mM
21	TCEP		additive	2 mM
22	TSP		internal reference	2 mM
23	NaN3		antimicrobial agent	0.01 %
24	D2O		solvent	10 %

8 HNHA

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (900 MHz)

Sample #1

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MAN1655-775	[U-99% 15N; U-10% 2H]	protein	0.8 mM
2	Phosphate		buffer	50 mM
3	NaCl		salt	150 mM
4	EDTA		chelating agent	1 mM
5	TCEP		additive	2 mM
6	TSP		internal reference	2 mM
7	NaN3		antimicrobial agent	0.01 %
8	D2O		solvent	10 %

Sample #2

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	MAN1655-775	[U-99% 15N; U-99% 13C; U10% 2H]	protein	0.8 mM
10	Phosphate		buffer	50 mM
11	NaCl		salt	150 mM
12	EDTA		chelating agent	1 mM
13	TCEP		additive	2 mM
14	TSP		internal reference	2 mM
15	NaN3		antimicrobial agent	0.01 %
16	D2O		solvent	10 %

Sample #3

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
17	MAN1655-775	[U-99% 15N; U-99% 13C; U-100% 2H]	protein	0.8 mM
18	Phosphate		buffer	50 mM
19	NaCl		salt	150 mM
20	EDTA		chelating agent	1 mM
21	TCEP		additive	2 mM
22	TSP		internal reference	2 mM
23	NaN3		antimicrobial agent	0.01 %
24	D2O		solvent	10 %

9 HBHA(CO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (900 MHz)

Sample #1

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MAN1655-775	[U-99% 15N; U-10% 2H]	protein	0.8 mM
2	Phosphate		buffer	50 mM
3	NaCl		salt	150 mM
4	EDTA		chelating agent	1 mM
5	TCEP		additive	2 mM
6	TSP		internal reference	2 mM
7	NaN3		antimicrobial agent	0.01 %
8	D2O		solvent	10 %

Sample #2

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	MAN1655-775	[U-99% 15N; U-99% 13C; U10% 2H]	protein	0.8 mM
10	Phosphate		buffer	50 mM
11	NaCl		salt	150 mM
12	EDTA		chelating agent	1 mM
13	TCEP		additive	2 mM
14	TSP		internal reference	2 mM
15	NaN3		antimicrobial agent	0.01 %
16	D2O		solvent	10 %

Sample #3

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
17	MAN1655-775	[U-99% 15N; U-99% 13C; U-100% 2H]	protein	0.8 mM
18	Phosphate		buffer	50 mM
19	NaCl		salt	150 mM
20	EDTA		chelating agent	1 mM
21	TCEP		additive	2 mM
22	TSP		internal reference	2 mM
23	NaN3		antimicrobial agent	0.01 %
24	D2O		solvent	10 %

10 HCCH-TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (900 MHz)

Sample #1

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MAN1655-775	[U-99% 15N; U-10% 2H]	protein	0.8 mM
2	Phosphate		buffer	50 mM
3	NaCl		salt	150 mM
4	EDTA		chelating agent	1 mM
5	TCEP		additive	2 mM
6	TSP		internal reference	2 mM
7	NaN3		antimicrobial agent	0.01 %
8	D2O		solvent	10 %

Sample #2

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	MAN1655-775	[U-99% 15N; U-99% 13C; U10% 2H]	protein	0.8 mM
10	Phosphate		buffer	50 mM
11	NaCl		salt	150 mM
12	EDTA		chelating agent	1 mM
13	TCEP		additive	2 mM
14	TSP		internal reference	2 mM
15	NaN3		antimicrobial agent	0.01 %
16	D2O		solvent	10 %

Sample #3

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
17	MAN1655-775	[U-99% 15N; U-99% 13C; U-100% 2H]	protein	0.8 mM
18	Phosphate		buffer	50 mM
19	NaCl		salt	150 mM
20	EDTA		chelating agent	1 mM
21	TCEP		additive	2 mM
22	TSP		internal reference	2 mM
23	NaN3		antimicrobial agent	0.01 %
24	D2O		solvent	10 %

11 HCCH-COSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (900 MHz)

Sample #1

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MAN1655-775	[U-99% 15N; U-10% 2H]	protein	0.8 mM
2	Phosphate		buffer	50 mM
3	NaCl		salt	150 mM
4	EDTA		chelating agent	1 mM
5	TCEP		additive	2 mM
6	TSP		internal reference	2 mM
7	NaN3		antimicrobial agent	0.01 %
8	D2O		solvent	10 %

Sample #2

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	MAN1655-775	[U-99% 15N; U-99% 13C; U10% 2H]	protein	0.8 mM
10	Phosphate		buffer	50 mM
11	NaCl		salt	150 mM
12	EDTA		chelating agent	1 mM
13	TCEP		additive	2 mM
14	TSP		internal reference	2 mM
15	NaN3		antimicrobial agent	0.01 %
16	D2O		solvent	10 %

Sample #3

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
17	MAN1655-775	[U-99% 15N; U-99% 13C; U-100% 2H]	protein	0.8 mM
18	Phosphate		buffer	50 mM
19	NaCl		salt	150 mM
20	EDTA		chelating agent	1 mM
21	TCEP		additive	2 mM
22	TSP		internal reference	2 mM
23	NaN3		antimicrobial agent	0.01 %
24	D2O		solvent	10 %

12 13C_HSQC_NOESY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (900 MHz)

Sample #1

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MAN1655-775	[U-99% 15N; U-10% 2H]	protein	0.8 mM
2	Phosphate		buffer	50 mM
3	NaCl		salt	150 mM
4	EDTA		chelating agent	1 mM
5	TCEP		additive	2 mM
6	TSP		internal reference	2 mM
7	NaN3		antimicrobial agent	0.01 %
8	D2O		solvent	10 %

Sample #2

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	MAN1655-775	[U-99% 15N; U-99% 13C; U10% 2H]	protein	0.8 mM
10	Phosphate		buffer	50 mM
11	NaCl		salt	150 mM
12	EDTA		chelating agent	1 mM
13	TCEP		additive	2 mM
14	TSP		internal reference	2 mM
15	NaN3		antimicrobial agent	0.01 %
16	D2O		solvent	10 %

Sample #3

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
17	MAN1655-775	[U-99% 15N; U-99% 13C; U-100% 2H]	protein	0.8 mM
18	Phosphate		buffer	50 mM
19	NaCl		salt	150 mM
20	EDTA		chelating agent	1 mM
21	TCEP		additive	2 mM
22	TSP		internal reference	2 mM
23	NaN3		antimicrobial agent	0.01 %
24	D2O		solvent	10 %

13 15N_HSQC-NOESY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (900 MHz)

Sample #1

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MAN1655-775	[U-99% 15N; U-10% 2H]	protein	0.8 mM
2	Phosphate		buffer	50 mM
3	NaCl		salt	150 mM
4	EDTA		chelating agent	1 mM
5	TCEP		additive	2 mM
6	TSP		internal reference	2 mM
7	NaN3		antimicrobial agent	0.01 %
8	D2O		solvent	10 %

Sample #2

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	MAN1655-775	[U-99% 15N; U-99% 13C; U10% 2H]	protein	0.8 mM
10	Phosphate		buffer	50 mM
11	NaCl		salt	150 mM
12	EDTA		chelating agent	1 mM
13	TCEP		additive	2 mM
14	TSP		internal reference	2 mM
15	NaN3		antimicrobial agent	0.01 %
16	D2O		solvent	10 %

Sample #3

Temperature 300 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
17	MAN1655-775	[U-99% 15N; U-99% 13C; U-100% 2H]	protein	0.8 mM
18	Phosphate		buffer	50 mM
19	NaCl		salt	150 mM
20	EDTA		chelating agent	1 mM
21	TCEP		additive	2 mM
22	TSP		internal reference	2 mM
23	NaN3		antimicrobial agent	0.01 %
24	D2O		solvent	10 %

nullKeywords MAN1, NMR, nuclear envelope, Smad, Structure, structure

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (2.864 seconds)

BMRB: 6919

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. MAN1 655-775 domain, : 1 : 6 entities Detail

Interaction partners 1. MAN1 655-775 domain, : 20 interactors Detail

Experiments performed 13 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2