BMRB: 6920

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Mid1 BBox 1

Authors

Massiah, M.A., Simmons, B.N., Short, K.M., Cox, T.C.

Assembly

Midline-1 B-box Type 1

Entity

1. B-box 1 type 1 (polymer, Thiol state: all other bound), 78 monomers, 8618.570 Da Detail

QKASVSGPNS PSETRRERAF DANTMTSAEK VLCQFCDQDP AQDAVKTCVT CEVSYCDECL KATHPNKKPF TGHRLIEP

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

8749.389 Da

Max. entity weight

8618.57 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	2:ZN1:ZN	1:CYS33:SG
2	na	sing	2:ZN1:ZN	1:CYS36:SG
3	na	sing	2:ZN1:ZN	1:CYS56:SG
4	na	sing	2:ZN1:ZN	1:CYS59:SG
5	na	sing	2:ZN1:ZN	1:CYS48:SG
6	na	sing	2:ZN1:ZN	1:CYS51:SG
7	na	sing	2:ZN1:ZN	1:HIS64:NE2
8	na	sing	2:ZN1:ZN	1:HIS73:HD1

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 71.2 %, Completeness (bb): 81.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	71.2 % (617 of 867)	79.7 % (362 of 454)	54.8 % (183 of 334)	91.1 % (72 of 79)
Backbone	81.4 % (371 of 456)	95.4 % (145 of 152)	67.7 % (157 of 232)	95.8 % (69 of 72)
Sidechain	63.9 % (311 of 487)	70.2 % (212 of 302)	54.5 % (97 of 178)	28.6 % (2 of 7)
Aromatic	28.3 % (13 of 46)	56.5 % (13 of 23)	0.0 % (0 of 23)
Methyl	79.7 % (51 of 64)	90.6 % (29 of 32)	68.8 % (22 of 32)

1. B-box 1 type 1

QKASVSGPNS PSETRRERAF DANTMTSAEK VLCQFCDQDP AQDAVKTCVT CEVSYCDECL KATHPNKKPF TGHRLIEP

Show sample condition

Sample

Temperature 294 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	B-box 1 type 1	[U-13C; U-15N]	protein	0.75 (±0.1) mM
2	ZINC (II) ION		ligand	0.0 ~ 0.0 mM
3	Tris			50 mM
4	NaCl			300 mM

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LACS Plot; CA

Referencing offset: -0.3 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.3 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 13 models in PDB: 2FFW, Strand ID: A Detail

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Release date

2006-04-26

Citation

Solution structure of the RBCC/TRIM B-box1 domain of human MID1: B-box with a RING
Massiah, M.A., Simmons, B.N., Short, K.M., Cox, T.C.
J. Mol. Biol. (2006), 358, 532-545, PubMed 16529770 , DOI 10.1016/j.jmb.2006.02.009 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. B-box 1 type 1, : 1 : 2 : 5 : 33 entities Detail

Interaction partners 1. B-box 1 type 1, : 28 interactors Detail

Experiments performed 8 experiments Detail