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BMRB: 6922


6922
2FE9
Solution structure of the Vts1 SAM domain in the presence of RNA
Authors
Edwards, T.A., Butterwick, J.A., Palmer, A.G., Aggarwal, A.K.
Assembly
Vts1:RNA dimer
Entity
1. Vts1 (438-523) (polymer, Thiol state: not present), 86 monomers, 9863.418 Da Detail

SNSSMNPKSL TDPKLLKNIP MWLKSLRLHK YSDALSGTPW IELIYLDDET LEKKGVLALG ARRKLLKAFG IVIDYKERDL IDRSAY


2. TCE (polymer, Thiol state: not present), 13 monomers, 4211.490 Da Detail

GGCUCUGGCA GCC


Total weight
14074.908 Da
Max. entity weight
9863.418 Da
Source organism
Saccharomyces cerevisiae , Drosophila melanogaster
Exptl. method
NMR
Refine. method
Torsion angle dynamics with CNS
Data set
assigned_chemical_shifts, RDCs
Chem. Shift Complete
Sequence coverage: 85.9 %, Completeness: 75.4 %, Completeness (bb): 73.4 % Detail
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Polymer type: polypeptide(L) polyribonucleotide

Total1H13C15N
All75.4 % (951 of 1261)72.0 % (481 of 668)77.4 % (386 of 499)89.4 % (84 of 94)
Backbone73.4 % (478 of 651)62.8 % (157 of 250)75.9 % (242 of 319)96.3 % (79 of 82)
Sidechain78.9 % (546 of 692)76.1 % (318 of 418)85.1 % (223 of 262)41.7 % (5 of 12)
Aromatic38.1 % (48 of 126)39.7 % (25 of 63)38.9 % (21 of 54)22.2 % (2 of 9)
Methyl100.0 % (108 of 108)100.0 % (54 of 54)100.0 % (54 of 54)

1. Vts1 (438-523)

SNSSMNPKSL TDPKLLKNIP MWLKSLRLHK YSDALSGTPW IELIYLDDET LEKKGVLALG ARRKLLKAFG IVIDYKERDL IDRSAY

2. TCE

GGCUCUGGCA GCC

Show sample condition
Sample

Temperature 303 (±1) K, pH 6.25 (±0.05)


#NameIsotope labelingTypeConcentration
1Vts1 (438-523)[U-13C; U-15N]0.5 mM
2TCE0.5 mM

LACS Plot; CA
Referencing offset: -0.63 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.63 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.08 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.1 ppm, Outliers: 3 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2FE9, Strand ID: A Detail

RDC
60 RDC values in 1 lists
Field strength (1H) 500 MHz, Temperature 303 (±1) K, pH 6.25 (±0.05) Detail
Release date
2007-02-05
Citation
Solution structure of the Vts1 SAM domain in the presence of RNA
Edwards, T.A., Butterwick, J.A., Zeng, L., Gupta, Y.K., Wang, X., Wharton, R.P., Palmer, A.G., Aggarwal, A.K.
J. Mol. Biol. (2006), 356, 1065-1072, PubMed 16405996 , DOI 10.1016/j.jmb.2005.12.004 ,
Related entities 1. Vts1 (438-523), : 1 : 5 : 2 : 18 entities Detail
More details for 26 related entities
Related entities 2. TCE, : 5 entities Detail
More details for 5 related entities
Interaction partners 1. Vts1 (438-523), : 7 interactors Detail
More details for 7 interactors identified by 5 citations
Experiments performed 10 experiments Detail
nullKeywords SAM, Vts1