BMRB: 6924

1H chemical shifts for discrepin, a scorpion toxin that blocks IA currents of the voltage dependent K+ channels

Authors

Prochnicka-Chalufour, A., Corzo, G., Satake, H., Martin-Euaclaire, M., Murgia, A.R., Prestipino, G., D'Suze, G., Possani, L.D., Delepierre, M.

Assembly

discrepin monomer

Entity

1. discrepin monomer (polymer, Thiol state: all disulfide bound), 38 monomers, 4182.830 Da Detail

XIDTNVKCSG SSKCVKICID RYNTRGAKCI NGRCTCYP

Formula weight

4182.83 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS8:SG	1:CYS29:SG
2	disulfide	sing	1:CYS14:SG	1:CYS34:SG
3	disulfide	sing	1:CYS18:SG	1:CYS36:SG

Source organism

Tityus discrepans

Exptl. method

NMR

Refine. method

Torsion Angle Dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 94.9 %, Completeness (bb): 97.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	94.9 % (203 of 214)	94.9 % (203 of 214)
Backbone	97.4 % (74 of 76)	97.4 % (74 of 76)
Sidechain	93.5 % (129 of 138)	93.5 % (129 of 138)
Aromatic	100.0 % (8 of 8)	100.0 % (8 of 8)
Methyl	100.0 % (16 of 16)	100.0 % (16 of 16)

1. discrepin, Tityus discrepans toxin from alpha-KTx15 sub-family

XIDTNVKCSG SSKCVKICID RYNTRGAKCI NGRCTCYP

Show sample condition

Sample

Temperature 308 (±0.5) K, pH 3 (±0.5), Details discrepin, H2O,D2O

#	Name	Isotope labeling	Type	Concentration
1	discrepin		protein	3.0 mM
2	H20		buffer	90 %
3	D20		buffer	10 %

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Protein Blocks Logo

Calculated from 10 models in PDB: 2AXK, Strand ID: A Detail

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Release date

2006-04-04

Citation 1

Discrepin, a new peptide of the sub-family alpha-ktx15, isolated from the scorpion Tityus discrepans irreversibly blocks K+ -channels (IA currents) of cerebellum granular cells
D'Suze, G., Batista, C.V.F., Frau, A., Murgia, A.R., Zamudio, F.Z., Sevcik, C., Possani, L.D., Possani, L.D., Prestipino, G.
Arch. Biochem. Biophys. (2004), 430, 256-263, PubMed 15369825 , DOI 10.1016/j.abb.2004.07.010 , Abstract

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Citation 2

Solution structure of discrepin, a new K+-channel blocking peptide from the alpha-KTx15 subfamily
Prochnicka-Chalufour, A., Corzo, G., Satake, H., Martin-Eauclaire, M., Murgia, A.R., Prestipino, G., D'Suze, G., Possani, L.D., Delepierre, M.
Biochemistry (2006), 45, 1795-1804, PubMed 16460026 , DOI 10.1021/bi0519248 , Abstract

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Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. discrepin monomer, : 1 : 1 : 143 entities Detail

Experiments performed 3 experiments Detail