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BMRB: 6925

2C06

Backbone and Beta 1H, 13C, and 15N Chemical Shift Assignments for the Bacterial Toxin Kid

Authors

Kamphuis, M.B., Boelens, R.

Assembly

Kid toxin

Entity

1. Kid toxin (polymer, Thiol state: all free), 110 monomers, 11880.63 Da Detail

MERGEIWLVS LDPTAGHEQQ GTRPVLIVTP AAFNRVTRLP VVVPVTSGGN FARTAGFAVS LDGVGIRTTG VVRCDQPRTI DMKARGGKRL ERVPETIMNE VLGRLSTILT

Formula weight

11880.63 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

HADDOCK

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 67.4 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	67.4 % (828 of 1229)	63.1 % (401 of 635)	66.9 % (324 of 484)	93.6 % (103 of 110)
Backbone	98.1 % (634 of 646)	98.7 % (222 of 225)	97.5 % (310 of 318)	99.0 % (102 of 103)
Sidechain	43.0 % (293 of 681)	43.9 % (180 of 410)	42.4 % (112 of 264)	14.3 % (1 of 7)
Aromatic	0.0 % (0 of 46)	0.0 % (0 of 23)	0.0 % (0 of 22)	0.0 % (0 of 1)
Methyl	17.5 % (27 of 154)	19.5 % (15 of 77)	15.6 % (12 of 77)

1. Kid toxin

MERGEIWLVS LDPTAGHEQQ GTRPVLIVTP AAFNRVTRLP VVVPVTSGGN FARTAGFAVS LDGVGIRTTG VVRCDQPRTI DMKARGGKRL ERVPETIMNE VLGRLSTILT

Show sample condition

Sample

Temperature 303 (±0.1) K, pH 5.8 (±0.01), Details 100 mM PO4 (K) pH 5.8

#	Name	Isotope labeling	Type	Concentration
1	Kid toxin	[U-13C; U-15N]		0.4 mM
2	PO4 (K)			100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.27 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.27 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2C06, Strand ID: A, B Detail

Release date

2006-11-05

Citation

Model for RNA binding and the catalytic site of the RNase Kid of the bacterial parD toxin-antitoxin system
Kamphuis, M.B., Bonvin, A.M., Monti, M.C., Lemonnier, M., Munoz-Gomez, A., van den Heuvel, R.H., Diaz-Orejas, R., Boelens, R.
J. Mol. Biol. (2006), 357, 115-126, PubMed 16413033 , DOI 10.1016/j.jmb.2005.12.033 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: P33647 released on 1994-02-01
    Title CHPB_ECOLI Entity mRNA interferase ChpB
  Swiss-Prot: P08365 released on 1988-08-01
    Title CHPS_ECOLI Entity Antitoxin ChpS

Related entities 1. Kid toxin, : 1 : 2 : 50 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC