BMRBj - BMREntryMaster

Found 1 Document (1.612 seconds)

BMRB: 6926

2FFT

Chemical Shift Assignments of Thylakoid Soluble Phosphoprotein of 9 kDa

Authors

Song, J., Carlberg, I., Lee, M.S., Markley, J.L.

Assembly

TSP9

Entity

1. TSP9 (polymer, Thiol state: not present), 84 monomers, 8726.685 Da Detail

SAAKGTAETK QEKSFVDWLL GKITKEDQFY ETDPILRGGD VKSSGSTSGK KGGTTSGKKG TVSIPSKKKN GNGGVFGGLF AKKD

Formula weight

8726.685 Da

Source organism

Spinacia oleracea

Exptl. method

Refine. method

torsion angle dynamics,simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 79.7 %, Completeness (bb): 93.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.7 % (749 of 940)	71.0 % (350 of 493)	86.9 % (313 of 360)	98.9 % (86 of 87)
Backbone	93.6 % (468 of 500)	91.2 % (165 of 181)	94.1 % (223 of 237)	97.6 % (80 of 82)
Sidechain	67.6 % (344 of 509)	59.0 % (184 of 312)	80.7 % (155 of 192)	100.0 % (5 of 5)
Aromatic	38.3 % (23 of 60)	73.3 % (22 of 30)	0.0 % (0 of 29)	100.0 % (1 of 1)
Methyl	88.2 % (60 of 68)	85.3 % (29 of 34)	91.2 % (31 of 34)

1. TSP9

SAAKGTAETK QEKSFVDWLL GKITKEDQFY ETDPILRGGD VKSSGSTSGK KGGTTSGKKG TVSIPSKKKN GNGGVFGGLF AKKD

Show sample condition

Sample

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1), Details 10 mM HEPES, 100 mM NaCl, and 75 mM SDS

#	Name	Isotope labeling	Type	Concentration
1	TSP9	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	HEPES		buffer	10 (±1.0) mM
3	NaCl		salt	100 (±5.0) mM
4	SDS		lipid	75 (±5.0) mM

LACS Plot; CA

Referencing offset: -0.68 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.68 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2FFT, Strand ID: A Detail

Release date

2005-12-22

Citation

Micelle-induced folding of spinach thylakoid soluble phosphoprotein of 9 kDa and its functional implications
Song, J., Lee, M.S., Carlberg, I., Vener, A.V., Markley, J.L.
Biochemistry (2006), 45, 15633-15643, PubMed 17176085 , DOI 10.1021/bi062148m , Abstract

Related entities 1. TSP9, : 1 : 3 entities Detail

Experiments performed 11 experiments Detail

1 1H15N_HSQC

Instrument

Varian Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1), Details 10 mM HEPES, 100 mM NaCl, and 75 mM SDS

#	Name	Isotope labeling	Type	Concentration
1	TSP9	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	HEPES		buffer	10 (±1.0) mM
3	NaCl		salt	100 (±5.0) mM
4	SDS		lipid	75 (±5.0) mM

2 1H,13C-HSQC

Instrument

Varian Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1), Details 10 mM HEPES, 100 mM NaCl, and 75 mM SDS

#	Name	Isotope labeling	Type	Concentration
1	TSP9	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	HEPES		buffer	10 (±1.0) mM
3	NaCl		salt	100 (±5.0) mM
4	SDS		lipid	75 (±5.0) mM

3 HNCACB

Instrument

Varian Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1), Details 10 mM HEPES, 100 mM NaCl, and 75 mM SDS

#	Name	Isotope labeling	Type	Concentration
1	TSP9	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	HEPES		buffer	10 (±1.0) mM
3	NaCl		salt	100 (±5.0) mM
4	SDS		lipid	75 (±5.0) mM

4 CBCACONH

Instrument

Varian Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1), Details 10 mM HEPES, 100 mM NaCl, and 75 mM SDS

#	Name	Isotope labeling	Type	Concentration
1	TSP9	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	HEPES		buffer	10 (±1.0) mM
3	NaCl		salt	100 (±5.0) mM
4	SDS		lipid	75 (±5.0) mM

5 CCONH

Instrument

Varian Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1), Details 10 mM HEPES, 100 mM NaCl, and 75 mM SDS

#	Name	Isotope labeling	Type	Concentration
1	TSP9	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	HEPES		buffer	10 (±1.0) mM
3	NaCl		salt	100 (±5.0) mM
4	SDS		lipid	75 (±5.0) mM

6 HCCONH

Instrument

Varian Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1), Details 10 mM HEPES, 100 mM NaCl, and 75 mM SDS

#	Name	Isotope labeling	Type	Concentration
1	TSP9	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	HEPES		buffer	10 (±1.0) mM
3	NaCl		salt	100 (±5.0) mM
4	SDS		lipid	75 (±5.0) mM

7 HCCHTOCSY

Instrument

Varian Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1), Details 10 mM HEPES, 100 mM NaCl, and 75 mM SDS

#	Name	Isotope labeling	Type	Concentration
1	TSP9	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	HEPES		buffer	10 (±1.0) mM
3	NaCl		salt	100 (±5.0) mM
4	SDS		lipid	75 (±5.0) mM

8 HNCO

Instrument

Varian Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1), Details 10 mM HEPES, 100 mM NaCl, and 75 mM SDS

#	Name	Isotope labeling	Type	Concentration
1	TSP9	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	HEPES		buffer	10 (±1.0) mM
3	NaCl		salt	100 (±5.0) mM
4	SDS		lipid	75 (±5.0) mM

9 HBACONH

Instrument

Varian Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1), Details 10 mM HEPES, 100 mM NaCl, and 75 mM SDS

#	Name	Isotope labeling	Type	Concentration
1	TSP9	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	HEPES		buffer	10 (±1.0) mM
3	NaCl		salt	100 (±5.0) mM
4	SDS		lipid	75 (±5.0) mM

10 3D 15N edited 1H,1H NOESY

Instrument

Varian Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1), Details 10 mM HEPES, 100 mM NaCl, and 75 mM SDS

#	Name	Isotope labeling	Type	Concentration
1	TSP9	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	HEPES		buffer	10 (±1.0) mM
3	NaCl		salt	100 (±5.0) mM
4	SDS		lipid	75 (±5.0) mM

11 3D 13C edited 1H,1H NOESY

Instrument

Varian Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1), Details 10 mM HEPES, 100 mM NaCl, and 75 mM SDS

#	Name	Isotope labeling	Type	Concentration
1	TSP9	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	HEPES		buffer	10 (±1.0) mM
3	NaCl		salt	100 (±5.0) mM
4	SDS		lipid	75 (±5.0) mM

null