BMRB: 6927

Spinophilin PDZ domain

Authors

Kelker, M.S., Peti, W.

Assembly

spinophlin PDZ domain

Entity

1. spinophlin PDZ domain (polymer, Thiol state: not present), 113 monomers, 12122.63 Da Detail

GHMELFPVEL EKDSEGLGIS IIGMGAGADM GLEKLGIFVK TVTEGGAAHR DGRIQVNDLL VEVDGTSLVG VTQSFAASVL RNTKGRVRFM IGRERPGEQS EVAQLIQQTL EQE

Formula weight

12122.63 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

simulated annealing torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 93.3 %, Completeness (bb): 96.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.3 % (1173 of 1257)	94.2 % (613 of 651)	92.6 % (450 of 486)	91.7 % (110 of 120)
Backbone	96.9 % (653 of 674)	97.1 % (233 of 240)	96.9 % (313 of 323)	96.4 % (107 of 111)
Sidechain	90.7 % (617 of 680)	92.5 % (380 of 411)	90.0 % (234 of 260)	33.3 % (3 of 9)
Aromatic	37.5 % (18 of 48)	66.7 % (16 of 24)	8.3 % (2 of 24)
Methyl	100.0 % (142 of 142)	100.0 % (71 of 71)	100.0 % (71 of 71)

1. spinophilin

GHMELFPVEL EKDSEGLGIS IIGMGAGADM GLEKLGIFVK TVTEGGAAHR DGRIQVNDLL VEVDGTSLVG VTQSFAASVL RNTKGRVRFM IGRERPGEQS EVAQLIQQTL EQE

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 6.5 (±0.2), Details 20 mM Na phohphate buffer, pH 6.5 50 mM NaCl 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	spinophilin		protein	2.0 mM
2	Na phohphate buffer		buffer	20 mM
3	NaCl		salt	50 mM
4	NaN3			0.02 %

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.18 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2G5M, Strand ID: B Detail

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Release date

2006-05-01

Citation

NMR assignment of the spinophilin PDZ domain (493-602)
Kelker, M.S., Peti, W.
J. Biomol. NMR (2006), 36 Suppl 1, 24-24, PubMed 16607467 , DOI 10.1007/s10858-006-0006-x , Abstract

Related entities 1. spinophlin PDZ domain, : 1 : 330 entities Detail

Interaction partners 1. spinophlin PDZ domain, : 43 interactors Detail

Experiments performed 6 experiments Detail