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Complete Chemical Shift Assignment for the Neurabin PDZ Domain
Authors
Dancheck, B., Peti, W.
Assembly
Neurabin PDZ
Entity
1. Neurabin PDZ (polymer, Thiol state: not present), 94 monomers, 9938.184 Da Detail

GHMELFPVEL EKDEDGLGIS IIGMGVGADA GLEKLGIFVK TVTEGGAAQR DGRIQVNDQI VEVDGISLVG VTQNFAATVL RNTKGNVRFV IGRE


Formula weight
9938.184 Da
Source organism
Rattus norvegicus
Exptl. method
NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.9 %, Completeness: 94.2 %, Completeness (bb): 97.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All94.2 % (976 of 1036)95.5 % (508 of 532)91.6 % (369 of 403)98.0 % (99 of 101)
Backbone97.0 % (545 of 562)97.5 % (197 of 202)96.3 % (257 of 267)97.8 % (91 of 93)
Sidechain91.9 % (508 of 553)94.2 % (311 of 330)87.9 % (189 of 215)100.0 % (8 of 8)
Aromatic43.2 % (19 of 44)86.4 % (19 of 22) 0.0 % (0 of 22)
Methyl100.0 % (130 of 130)100.0 % (65 of 65)100.0 % (65 of 65)

1. Neurabin PDZ Domain

GHMELFPVEL EKDEDGLGIS IIGMGVGADA GLEKLGIFVK TVTEGGAAQR DGRIQVNDQI VEVDGISLVG VTQNFAATVL RNTKGNVRFV IGRE

Sample

Temperature 298 (±0.2) K, pH 6.5 (±0.1)


#NameIsotope labelingTypeConcentration
1Neurabin PDZ Domainprotein2 mM
2Na phosphatebuffer20 mM
3NaClsalt50 mM

LACS Plot; CA
Referencing offset: -0.17 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.17 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.04 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.17 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2FN5, Strand ID: A Detail


Release date
2007-04-10
Citation
Structural basis for spinophilin-neurabin receptor interaction
Kelker, M.S., Dancheck, B., Ju, T., Kessler, R.P., Hudak, J., Nairn, A.C., Peti, W.
Biochemistry (2007), 46, 2333-2344, PubMed 17279777 , DOI 10.1021/bi602341c ,
Related entities 1. Neurabin PDZ, : 1 : 583 entities Detail
Interaction partners 1. Neurabin PDZ, : 3 interactors Detail
Experiments performed 7 experiments Detail
nullKeywords Neurabin, PDZ