BMRBj - BMREntryMaster

Found 1 Document (2.123 seconds)

BMRB: 6934

2FEK

1H, 15N, and 13C Assigned Chemical shift for Wzb

Authors

Lescop, E., Jin, C.

Assembly

Wzb monomer

Entity

1. Wzb monomer (polymer, Thiol state: all free), 167 monomers, 18872.41 Da Detail

MGSSHHHHHH SSGLVPRGSH MFNNILVVCV GNICRSPTAE RLLQRYHPEL KVESAGLGAL VGKGADPTAI SVAAEHQLSL EGHCARQISR RLCRNYDLIL TMEKRHIERL CEMAPEMRGK VMLFGHWDNE CEIPDPYRKS RETFAAVYTL LERSARQWAQ ALNAEQV

Formula weight

18872.41 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

200 structures were generated in CYANA. The 100 structures with lowest energy were further refined in AMBER7. The 20 lowest energy structures were finally selected.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.2 %, Completeness: 84.4 %, Completeness (bb): 87.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.4 % (1617 of 1916)	86.5 % (864 of 999)	80.5 % (598 of 743)	89.1 % (155 of 174)
Backbone	87.6 % (865 of 988)	87.6 % (296 of 338)	87.1 % (427 of 490)	88.7 % (142 of 160)
Sidechain	82.3 % (892 of 1084)	85.9 % (568 of 661)	76.0 % (311 of 409)	92.9 % (13 of 14)
Aromatic	29.1 % (39 of 134)	53.7 % (36 of 67)	3.1 % (2 of 65)	50.0 % (1 of 2)
Methyl	95.5 % (168 of 176)	95.5 % (84 of 88)	95.5 % (84 of 88)

1. Wzb

MGSSHHHHHH SSGLVPRGSH MFNNILVVCV GNICRSPTAE RLLQRYHPEL KVESAGLGAL VGKGADPTAI SVAAEHQLSL EGHCARQISR RLCRNYDLIL TMEKRHIERL CEMAPEMRGK VMLFGHWDNE CEIPDPYRKS RETFAAVYTL LERSARQWAQ ALNAEQV

Show sample condition

Sample #1

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Wzb	[U-95% 13C; U-95% 15N]	1 mM
2	sodium phosphate		50 mM
3	sodium chloride		50 mM
4	DTT		25 mM
5	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
6	Wzb	[U-95% 15N]	1 mM
7	sodium phosphate		50 mM
8	sodium chloride		50 mM
9	DTT		25 mM
10	sodium azide		0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2FEK, Strand ID: A Detail

Release date

2007-02-05

Citation

The solution structure of Escherichia coli Wzb reveals a novel substrate recognition mechanism of prokaryotic low molecular weight protein-tyrosine phosphatases
Lescop, E., Hu, Y., Xu, H., Hu, W., Chen, J., Xia, B., Jin, C.
J. Biol. Chem. (2006), 281, 19570-19577, PubMed 16651264 , DOI 10.1074/jbc.M601263200 , Abstract

Related entities 1. Wzb monomer, : 1 : 2 : 82 entities Detail

Interaction partners 1. Wzb monomer, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 1H-15N_HSQC

Instrument

#1 Model Bruker Avance (800 MHz) Details equipped with triple-resonance probes and pulsed-field gradients

#2 Model Bruker Avance (500 MHz) Details with cryoprobe and equipped with triple-resonance probes and pulsed-field gradients

Sample #1

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Wzb	[U-95% 13C; U-95% 15N]	1 mM
2	sodium phosphate		50 mM
3	sodium chloride		50 mM
4	DTT		25 mM
5	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
6	Wzb	[U-95% 15N]	1 mM
7	sodium phosphate		50 mM
8	sodium chloride		50 mM
9	DTT		25 mM
10	sodium azide		0.02 %

2 1H-13C_HSQC

Instrument

#1 Model Bruker Avance (800 MHz) Details equipped with triple-resonance probes and pulsed-field gradients

#2 Model Bruker Avance (500 MHz) Details with cryoprobe and equipped with triple-resonance probes and pulsed-field gradients

Sample #1

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Wzb	[U-95% 13C; U-95% 15N]	1 mM
2	sodium phosphate		50 mM
3	sodium chloride		50 mM
4	DTT		25 mM
5	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
6	Wzb	[U-95% 15N]	1 mM
7	sodium phosphate		50 mM
8	sodium chloride		50 mM
9	DTT		25 mM
10	sodium azide		0.02 %

3 HNCA

Instrument

#1 Model Bruker Avance (800 MHz) Details equipped with triple-resonance probes and pulsed-field gradients

#2 Model Bruker Avance (500 MHz) Details with cryoprobe and equipped with triple-resonance probes and pulsed-field gradients

Sample #1

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Wzb	[U-95% 13C; U-95% 15N]	1 mM
2	sodium phosphate		50 mM
3	sodium chloride		50 mM
4	DTT		25 mM
5	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
6	Wzb	[U-95% 15N]	1 mM
7	sodium phosphate		50 mM
8	sodium chloride		50 mM
9	DTT		25 mM
10	sodium azide		0.02 %

4 HNCO

Instrument

#1 Model Bruker Avance (800 MHz) Details equipped with triple-resonance probes and pulsed-field gradients

#2 Model Bruker Avance (500 MHz) Details with cryoprobe and equipped with triple-resonance probes and pulsed-field gradients

Sample #1

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Wzb	[U-95% 13C; U-95% 15N]	1 mM
2	sodium phosphate		50 mM
3	sodium chloride		50 mM
4	DTT		25 mM
5	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
6	Wzb	[U-95% 15N]	1 mM
7	sodium phosphate		50 mM
8	sodium chloride		50 mM
9	DTT		25 mM
10	sodium azide		0.02 %

5 HN(CA)CO

Instrument

#1 Model Bruker Avance (800 MHz) Details equipped with triple-resonance probes and pulsed-field gradients

#2 Model Bruker Avance (500 MHz) Details with cryoprobe and equipped with triple-resonance probes and pulsed-field gradients

Sample #1

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Wzb	[U-95% 13C; U-95% 15N]	1 mM
2	sodium phosphate		50 mM
3	sodium chloride		50 mM
4	DTT		25 mM
5	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
6	Wzb	[U-95% 15N]	1 mM
7	sodium phosphate		50 mM
8	sodium chloride		50 mM
9	DTT		25 mM
10	sodium azide		0.02 %

6 CBCACONH

Instrument

#1 Model Bruker Avance (800 MHz) Details equipped with triple-resonance probes and pulsed-field gradients

#2 Model Bruker Avance (500 MHz) Details with cryoprobe and equipped with triple-resonance probes and pulsed-field gradients

Sample #1

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Wzb	[U-95% 13C; U-95% 15N]	1 mM
2	sodium phosphate		50 mM
3	sodium chloride		50 mM
4	DTT		25 mM
5	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
6	Wzb	[U-95% 15N]	1 mM
7	sodium phosphate		50 mM
8	sodium chloride		50 mM
9	DTT		25 mM
10	sodium azide		0.02 %

7 HNCACB

Instrument

#1 Model Bruker Avance (800 MHz) Details equipped with triple-resonance probes and pulsed-field gradients

#2 Model Bruker Avance (500 MHz) Details with cryoprobe and equipped with triple-resonance probes and pulsed-field gradients

Sample #1

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Wzb	[U-95% 13C; U-95% 15N]	1 mM
2	sodium phosphate		50 mM
3	sodium chloride		50 mM
4	DTT		25 mM
5	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
6	Wzb	[U-95% 15N]	1 mM
7	sodium phosphate		50 mM
8	sodium chloride		50 mM
9	DTT		25 mM
10	sodium azide		0.02 %

8 CCH-TOCSY-HSQC

Instrument

#1 Model Bruker Avance (800 MHz) Details equipped with triple-resonance probes and pulsed-field gradients

#2 Model Bruker Avance (500 MHz) Details with cryoprobe and equipped with triple-resonance probes and pulsed-field gradients

Sample #1

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Wzb	[U-95% 13C; U-95% 15N]	1 mM
2	sodium phosphate		50 mM
3	sodium chloride		50 mM
4	DTT		25 mM
5	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
6	Wzb	[U-95% 15N]	1 mM
7	sodium phosphate		50 mM
8	sodium chloride		50 mM
9	DTT		25 mM
10	sodium azide		0.02 %

9 HCCH-TOCSY-HSQC

Instrument

#1 Model Bruker Avance (800 MHz) Details equipped with triple-resonance probes and pulsed-field gradients

#2 Model Bruker Avance (500 MHz) Details with cryoprobe and equipped with triple-resonance probes and pulsed-field gradients

Sample #1

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Wzb	[U-95% 13C; U-95% 15N]	1 mM
2	sodium phosphate		50 mM
3	sodium chloride		50 mM
4	DTT		25 mM
5	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
6	Wzb	[U-95% 15N]	1 mM
7	sodium phosphate		50 mM
8	sodium chloride		50 mM
9	DTT		25 mM
10	sodium azide		0.02 %

10 CCH-COSY-HSQC

Instrument

#1 Model Bruker Avance (800 MHz) Details equipped with triple-resonance probes and pulsed-field gradients

#2 Model Bruker Avance (500 MHz) Details with cryoprobe and equipped with triple-resonance probes and pulsed-field gradients

Sample #1

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Wzb	[U-95% 13C; U-95% 15N]	1 mM
2	sodium phosphate		50 mM
3	sodium chloride		50 mM
4	DTT		25 mM
5	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6 (±0.1)

#	Name	Isotope labeling	Concentration
6	Wzb	[U-95% 15N]	1 mM
7	sodium phosphate		50 mM
8	sodium chloride		50 mM
9	DTT		25 mM
10	sodium azide		0.02 %

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Found 1 Document (2.123 seconds)

BMRB: 6934

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Wzb monomer, : 1 : 2 : 82 entities Detail

Interaction partners 1. Wzb monomer, : 3 interactors Detail

Experiments performed 10 experiments Detail

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