1H, 15N, and 13C Assigned Chemical shift for Wzb
MGSSHHHHHH SSGLVPRGSH MFNNILVVCV GNICRSPTAE RLLQRYHPEL KVESAGLGAL VGKGADPTAI SVAAEHQLSL EGHCARQISR RLCRNYDLIL TMEKRHIERL CEMAPEMRGK VMLFGHWDNE CEIPDPYRKS RETFAAVYTL LERSARQWAQ ALNAEQV
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 84.4 % (1617 of 1916) | 86.5 % (864 of 999) | 80.5 % (598 of 743) | 89.1 % (155 of 174) |
Backbone | 87.6 % (865 of 988) | 87.6 % (296 of 338) | 87.1 % (427 of 490) | 88.7 % (142 of 160) |
Sidechain | 82.3 % (892 of 1084) | 85.9 % (568 of 661) | 76.0 % (311 of 409) | 92.9 % (13 of 14) |
Aromatic | 29.1 % (39 of 134) | 53.7 % (36 of 67) | 3.1 % (2 of 65) | 50.0 % (1 of 2) |
Methyl | 95.5 % (168 of 176) | 95.5 % (84 of 88) | 95.5 % (84 of 88) |
1. Wzb
MGSSHHHHHH SSGLVPRGSH MFNNILVVCV GNICRSPTAE RLLQRYHPEL KVESAGLGAL VGKGADPTAI SVAAEHQLSL EGHCARQISR RLCRNYDLIL TMEKRHIERL CEMAPEMRGK VMLFGHWDNE CEIPDPYRKS RETFAAVYTL LERSARQWAQ ALNAEQVTemperature 298 (±0.2) K, pH 6 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Wzb | [U-95% 13C; U-95% 15N] | 1 mM | |
2 | sodium phosphate | 50 mM | ||
3 | sodium chloride | 50 mM | ||
4 | DTT | 25 mM | ||
5 | sodium azide | 0.02 % |
Temperature 298 (±0.2) K, pH 6 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Wzb | [U-95% 15N] | 1 mM | |
7 | sodium phosphate | 50 mM | ||
8 | sodium chloride | 50 mM | ||
9 | DTT | 25 mM | ||
10 | sodium azide | 0.02 % |