BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	97.1 % (1372 of 1413)	96.3 % (721 of 749)	98.3 % (531 of 540)	96.8 % (120 of 124)
Backbone	98.6 % (651 of 660)	97.8 % (219 of 224)	99.7 % (327 of 328)	97.2 % (105 of 108)
Sidechain	96.3 % (827 of 859)	95.6 % (502 of 525)	97.5 % (310 of 318)	93.8 % (15 of 16)
Aromatic	90.8 % (138 of 152)	92.1 % (70 of 76)	89.0 % (65 of 73)	100.0 % (3 of 3)
Methyl	99.0 % (97 of 98)	98.0 % (48 of 49)	100.0 % (49 of 49)

1. K2

GQRQLLLASE NPQQFMDYFS EEFRNDFLEL LRRRFGTKRV HNNIVYNEYI SHREHIHMNA TQWETLTDFT KWLGREGLCK VDETPKGWYI QYIDRDPETI RRQLELEKKK K

Show sample condition

Sample #1

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N label

#	Name	Isotope labeling	Type	Concentration
1	KIN17	[U-15N]	protein	0.7 mM
2	NaCl		salt	150 mM
3	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
4	H2O		solvent	90 %
5	D2O			10 %
6	TCEP			1 mM

Sample #2

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label

#	Name	Isotope labeling	Type	Concentration
7	KIN17	[U-13C; U-15N]	protein	0.8 mM
8	NaCl		salt	150 mM
9	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
10	H2O		solvent	90 %
11	D2O			10 %
12	TCEP			1 mM

Sample #3

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label in D2O 100%

#	Name	Isotope labeling	Type	Concentration
13	KIN17	[U-13C; U-15N]	protein	0.7 mM
14	NaCl		salt	150 mM
15	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
16	D2O			100 %
17	TCEP			1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	internal	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	internal	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	internal	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	internal	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 12 models in PDB: 2V1N, Strand ID: A Detail

Release date

2006-05-01

Citation

NMR assignment of region 51-160 of human KIN17, a DNA and RNA-binding protein
Carlier, L., le Maire, A., Braud, S., Masson, C., Gondry, M., Zinn-Justin, S., Guilhaudis, L., Milazzo, I., Davoust, D., Gilquin, B., Couprie, J.
J. Biomol. NMR (2006), 36 Suppl 1, 29-29, PubMed 16636754 , DOI 10.1007/s10858-006-0013-y , Abstract

Related entities 1. monomer region 51-160 of human KIN17, : 1 : 5 : 20 entities Detail

Interaction partners 1. monomer region 51-160 of human KIN17, : 4 interactors Detail

More details for 4 interactors identified by 4 citations

Experiments performed 12 experiments Detail

1 1H-15N_HSQC

Instrument

#1 Model Bruker Avance DMX (600 MHz) Details 600 MHz equipped with triple resnance cryoprobe

#2 Model Varian Inova (800 MHz) Details 800 MHz equipped with triple resnance cryoprobe

Sample #1

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N label

#	Name	Isotope labeling	Type	Concentration
1	KIN17	[U-15N]	protein	0.7 mM
2	NaCl		salt	150 mM
3	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
4	H2O		solvent	90 %
5	D2O			10 %
6	TCEP			1 mM

Sample #2

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label

#	Name	Isotope labeling	Type	Concentration
7	KIN17	[U-13C; U-15N]	protein	0.8 mM
8	NaCl		salt	150 mM
9	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
10	H2O		solvent	90 %
11	D2O			10 %
12	TCEP			1 mM

Sample #3

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label in D2O 100%

#	Name	Isotope labeling	Type	Concentration
13	KIN17	[U-13C; U-15N]	protein	0.7 mM
14	NaCl		salt	150 mM
15	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
16	D2O			100 %
17	TCEP			1 mM

2 HNCO

Instrument

#1 Model Bruker Avance DMX (600 MHz) Details 600 MHz equipped with triple resnance cryoprobe

#2 Model Varian Inova (800 MHz) Details 800 MHz equipped with triple resnance cryoprobe

Sample #1

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N label

#	Name	Isotope labeling	Type	Concentration
1	KIN17	[U-15N]	protein	0.7 mM
2	NaCl		salt	150 mM
3	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
4	H2O		solvent	90 %
5	D2O			10 %
6	TCEP			1 mM

Sample #2

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label

#	Name	Isotope labeling	Type	Concentration
7	KIN17	[U-13C; U-15N]	protein	0.8 mM
8	NaCl		salt	150 mM
9	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
10	H2O		solvent	90 %
11	D2O			10 %
12	TCEP			1 mM

Sample #3

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label in D2O 100%

#	Name	Isotope labeling	Type	Concentration
13	KIN17	[U-13C; U-15N]	protein	0.7 mM
14	NaCl		salt	150 mM
15	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
16	D2O			100 %
17	TCEP			1 mM

3 HNCA

Instrument

#1 Model Bruker Avance DMX (600 MHz) Details 600 MHz equipped with triple resnance cryoprobe

#2 Model Varian Inova (800 MHz) Details 800 MHz equipped with triple resnance cryoprobe

Sample #1

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N label

#	Name	Isotope labeling	Type	Concentration
1	KIN17	[U-15N]	protein	0.7 mM
2	NaCl		salt	150 mM
3	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
4	H2O		solvent	90 %
5	D2O			10 %
6	TCEP			1 mM

Sample #2

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label

#	Name	Isotope labeling	Type	Concentration
7	KIN17	[U-13C; U-15N]	protein	0.8 mM
8	NaCl		salt	150 mM
9	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
10	H2O		solvent	90 %
11	D2O			10 %
12	TCEP			1 mM

Sample #3

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label in D2O 100%

#	Name	Isotope labeling	Type	Concentration
13	KIN17	[U-13C; U-15N]	protein	0.7 mM
14	NaCl		salt	150 mM
15	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
16	D2O			100 %
17	TCEP			1 mM

4 CBCACONH

Instrument

#1 Model Bruker Avance DMX (600 MHz) Details 600 MHz equipped with triple resnance cryoprobe

#2 Model Varian Inova (800 MHz) Details 800 MHz equipped with triple resnance cryoprobe

Sample #1

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N label

#	Name	Isotope labeling	Type	Concentration
1	KIN17	[U-15N]	protein	0.7 mM
2	NaCl		salt	150 mM
3	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
4	H2O		solvent	90 %
5	D2O			10 %
6	TCEP			1 mM

Sample #2

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label

#	Name	Isotope labeling	Type	Concentration
7	KIN17	[U-13C; U-15N]	protein	0.8 mM
8	NaCl		salt	150 mM
9	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
10	H2O		solvent	90 %
11	D2O			10 %
12	TCEP			1 mM

Sample #3

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label in D2O 100%

#	Name	Isotope labeling	Type	Concentration
13	KIN17	[U-13C; U-15N]	protein	0.7 mM
14	NaCl		salt	150 mM
15	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
16	D2O			100 %
17	TCEP			1 mM

5 CBCANH

Instrument

#1 Model Bruker Avance DMX (600 MHz) Details 600 MHz equipped with triple resnance cryoprobe

#2 Model Varian Inova (800 MHz) Details 800 MHz equipped with triple resnance cryoprobe

Sample #1

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N label

#	Name	Isotope labeling	Type	Concentration
1	KIN17	[U-15N]	protein	0.7 mM
2	NaCl		salt	150 mM
3	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
4	H2O		solvent	90 %
5	D2O			10 %
6	TCEP			1 mM

Sample #2

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label

#	Name	Isotope labeling	Type	Concentration
7	KIN17	[U-13C; U-15N]	protein	0.8 mM
8	NaCl		salt	150 mM
9	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
10	H2O		solvent	90 %
11	D2O			10 %
12	TCEP			1 mM

Sample #3

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label in D2O 100%

#	Name	Isotope labeling	Type	Concentration
13	KIN17	[U-13C; U-15N]	protein	0.7 mM
14	NaCl		salt	150 mM
15	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
16	D2O			100 %
17	TCEP			1 mM

6 (HCA)CO(CA)NH

Instrument

#1 Model Bruker Avance DMX (600 MHz) Details 600 MHz equipped with triple resnance cryoprobe

#2 Model Varian Inova (800 MHz) Details 800 MHz equipped with triple resnance cryoprobe

Sample #1

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N label

#	Name	Isotope labeling	Type	Concentration
1	KIN17	[U-15N]	protein	0.7 mM
2	NaCl		salt	150 mM
3	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
4	H2O		solvent	90 %
5	D2O			10 %
6	TCEP			1 mM

Sample #2

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label

#	Name	Isotope labeling	Type	Concentration
7	KIN17	[U-13C; U-15N]	protein	0.8 mM
8	NaCl		salt	150 mM
9	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
10	H2O		solvent	90 %
11	D2O			10 %
12	TCEP			1 mM

Sample #3

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label in D2O 100%

#	Name	Isotope labeling	Type	Concentration
13	KIN17	[U-13C; U-15N]	protein	0.7 mM
14	NaCl		salt	150 mM
15	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
16	D2O			100 %
17	TCEP			1 mM

7 HBHACONH

Instrument

#1 Model Bruker Avance DMX (600 MHz) Details 600 MHz equipped with triple resnance cryoprobe

#2 Model Varian Inova (800 MHz) Details 800 MHz equipped with triple resnance cryoprobe

Sample #1

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N label

#	Name	Isotope labeling	Type	Concentration
1	KIN17	[U-15N]	protein	0.7 mM
2	NaCl		salt	150 mM
3	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
4	H2O		solvent	90 %
5	D2O			10 %
6	TCEP			1 mM

Sample #2

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label

#	Name	Isotope labeling	Type	Concentration
7	KIN17	[U-13C; U-15N]	protein	0.8 mM
8	NaCl		salt	150 mM
9	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
10	H2O		solvent	90 %
11	D2O			10 %
12	TCEP			1 mM

Sample #3

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label in D2O 100%

#	Name	Isotope labeling	Type	Concentration
13	KIN17	[U-13C; U-15N]	protein	0.7 mM
14	NaCl		salt	150 mM
15	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
16	D2O			100 %
17	TCEP			1 mM

8 HCCH-COSY

Instrument

#1 Model Bruker Avance DMX (600 MHz) Details 600 MHz equipped with triple resnance cryoprobe

#2 Model Varian Inova (800 MHz) Details 800 MHz equipped with triple resnance cryoprobe

Sample #1

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N label

#	Name	Isotope labeling	Type	Concentration
1	KIN17	[U-15N]	protein	0.7 mM
2	NaCl		salt	150 mM
3	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
4	H2O		solvent	90 %
5	D2O			10 %
6	TCEP			1 mM

Sample #2

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label

#	Name	Isotope labeling	Type	Concentration
7	KIN17	[U-13C; U-15N]	protein	0.8 mM
8	NaCl		salt	150 mM
9	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
10	H2O		solvent	90 %
11	D2O			10 %
12	TCEP			1 mM

Sample #3

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label in D2O 100%

#	Name	Isotope labeling	Type	Concentration
13	KIN17	[U-13C; U-15N]	protein	0.7 mM
14	NaCl		salt	150 mM
15	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
16	D2O			100 %
17	TCEP			1 mM

9 HCCH-TOCSY

Instrument

#1 Model Bruker Avance DMX (600 MHz) Details 600 MHz equipped with triple resnance cryoprobe

#2 Model Varian Inova (800 MHz) Details 800 MHz equipped with triple resnance cryoprobe

Sample #1

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N label

#	Name	Isotope labeling	Type	Concentration
1	KIN17	[U-15N]	protein	0.7 mM
2	NaCl		salt	150 mM
3	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
4	H2O		solvent	90 %
5	D2O			10 %
6	TCEP			1 mM

Sample #2

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label

#	Name	Isotope labeling	Type	Concentration
7	KIN17	[U-13C; U-15N]	protein	0.8 mM
8	NaCl		salt	150 mM
9	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
10	H2O		solvent	90 %
11	D2O			10 %
12	TCEP			1 mM

Sample #3

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label in D2O 100%

#	Name	Isotope labeling	Type	Concentration
13	KIN17	[U-13C; U-15N]	protein	0.7 mM
14	NaCl		salt	150 mM
15	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
16	D2O			100 %
17	TCEP			1 mM

10 15N-NOESY-HSQC

Instrument

#1 Model Bruker Avance DMX (600 MHz) Details 600 MHz equipped with triple resnance cryoprobe

#2 Model Varian Inova (800 MHz) Details 800 MHz equipped with triple resnance cryoprobe

Sample #1

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N label

#	Name	Isotope labeling	Type	Concentration
1	KIN17	[U-15N]	protein	0.7 mM
2	NaCl		salt	150 mM
3	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
4	H2O		solvent	90 %
5	D2O			10 %
6	TCEP			1 mM

Sample #2

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label

#	Name	Isotope labeling	Type	Concentration
7	KIN17	[U-13C; U-15N]	protein	0.8 mM
8	NaCl		salt	150 mM
9	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
10	H2O		solvent	90 %
11	D2O			10 %
12	TCEP			1 mM

Sample #3

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label in D2O 100%

#	Name	Isotope labeling	Type	Concentration
13	KIN17	[U-13C; U-15N]	protein	0.7 mM
14	NaCl		salt	150 mM
15	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
16	D2O			100 %
17	TCEP			1 mM

11 13C-NOESY-HSQC

Instrument

#1 Model Bruker Avance DMX (600 MHz) Details 600 MHz equipped with triple resnance cryoprobe

#2 Model Varian Inova (800 MHz) Details 800 MHz equipped with triple resnance cryoprobe

Sample #1

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N label

#	Name	Isotope labeling	Type	Concentration
1	KIN17	[U-15N]	protein	0.7 mM
2	NaCl		salt	150 mM
3	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
4	H2O		solvent	90 %
5	D2O			10 %
6	TCEP			1 mM

Sample #2

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label

#	Name	Isotope labeling	Type	Concentration
7	KIN17	[U-13C; U-15N]	protein	0.8 mM
8	NaCl		salt	150 mM
9	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
10	H2O		solvent	90 %
11	D2O			10 %
12	TCEP			1 mM

Sample #3

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label in D2O 100%

#	Name	Isotope labeling	Type	Concentration
13	KIN17	[U-13C; U-15N]	protein	0.7 mM
14	NaCl		salt	150 mM
15	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
16	D2O			100 %
17	TCEP			1 mM

12 HNHA

Instrument

#1 Model Bruker Avance DMX (600 MHz) Details 600 MHz equipped with triple resnance cryoprobe

#2 Model Varian Inova (800 MHz) Details 800 MHz equipped with triple resnance cryoprobe

Sample #1

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N label

#	Name	Isotope labeling	Type	Concentration
1	KIN17	[U-15N]	protein	0.7 mM
2	NaCl		salt	150 mM
3	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
4	H2O		solvent	90 %
5	D2O			10 %
6	TCEP			1 mM

Sample #2

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label

#	Name	Isotope labeling	Type	Concentration
7	KIN17	[U-13C; U-15N]	protein	0.8 mM
8	NaCl		salt	150 mM
9	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
10	H2O		solvent	90 %
11	D2O			10 %
12	TCEP			1 mM

Sample #3

Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label in D2O 100%

#	Name	Isotope labeling	Type	Concentration
13	KIN17	[U-13C; U-15N]	protein	0.7 mM
14	NaCl		salt	150 mM
15	NaH2PO4/Na2HPO4 Phosphate		salt	50 mM
16	D2O			100 %
17	TCEP			1 mM

nullKeywords DNA-binding, KIN17, NMR assignments, nuclear metabolism, RNA-binding, NMR assignment

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BMRB: 6938

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. monomer region 51-160 of human KIN17, : 1 : 5 : 20 entities Detail

Interaction partners 1. monomer region 51-160 of human KIN17, : 4 interactors Detail

Experiments performed 12 experiments Detail

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