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BMRB: 6943

2JNT

Resonance Assignment of Bombyx mori Chemosensory Protein 1

Authors

Jansen, S., Zidek, L., Lofstedt, C., Picimbon, J., Sklenar, V.

Assembly

chemosensory protein 1

Entity

1. chemosensory protein 1 (polymer, Thiol state: all disulfide bound), 108 monomers, 12537.09 Da Detail

YTDKYDKINL QEILENKRLL ESYMDCVLGK GKCTPEGKEL KDHLQEALET GCEKCTEAQE KGAETSIDYL IKNELEIWKE LTAHFDPDGK WRKKYEDRAK AKGIVIPE

Formula weight

12537.09 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS26:SG	1:CYS33:SG
2	disulfide	sing	1:CYS52:SG	1:CYS55:SG

Source organism

Bombyx mori

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.9 %, Completeness (bb): 98.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.9 % (1262 of 1303)	96.7 % (666 of 689)	96.6 % (484 of 501)	99.1 % (112 of 113)
Backbone	98.8 % (634 of 642)	99.1 % (218 of 220)	98.1 % (311 of 317)	100.0 % (105 of 105)
Sidechain	95.7 % (729 of 762)	95.5 % (448 of 469)	96.1 % (274 of 285)	87.5 % (7 of 8)
Aromatic	73.2 % (60 of 82)	73.2 % (30 of 41)	74.4 % (29 of 39)	50.0 % (1 of 2)
Methyl	100.0 % (104 of 104)	100.0 % (52 of 52)	100.0 % (52 of 52)

1. chemosensory protein 1

YTDKYDKINL QEILENKRLL ESYMDCVLGK GKCTPEGKEL KDHLQEALET GCEKCTEAQE KGAETSIDYL IKNELEIWKE LTAHFDPDGK WRKKYEDRAK AKGIVIPE

Show sample condition

Sample

Temperature 293 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	chemosensory protein 1	[U-95% 13C; U-90% 15N]	protein	1.0 (±0.1) mM
2	sodium phosphate		buffer	20 mM
3	sodium azide		antimicrobial agent	0.05 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.43 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2JNT, Strand ID: A Detail

Release date

2006-08-06

Citation

1H, 13C, and 15N resonance assignment of Bombyx mori chemosensory protein 1 (BmorCSP1)
Jansen, S., Zidek, L., Lofstedt, C., Picimbon, J., Sklenar, V.
J. Biomol. NMR (2006), 36 Suppl 1, 47-47, PubMed 16819588 , DOI 10.1007/s10858-006-9019-8 , Abstract

Related entities 1. chemosensory protein 1, : 1 : 15 entities Detail

Experiments performed 8 experiments Detail

1 1H15N HSQC