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BMRB: 6946

2LKC

Solution structure of free form of the GTP/GDP binding domain of translation initiation factor IF2

Authors

Tischenko, E.V., Folkers, G., Boelens, R., Gualerzi, C.O.

Assembly

g2 free

Entity

1. g2 free (polymer, Thiol state: all free), 178 monomers, 19500.20 Da Detail

GSHMVERPPV VTIMGHVDHG KTTLLDAIRH SKVTEQEAGG ITQHIGAYQV TVNDKKITFL DTPGHEAFTT MRARGAQVTD IVILVVAADD GVMPQTVEAI NHAKAANVPI IVAINKMDKP EANPDRVMQE LMEYNLVPEE WGGDTIFCKL SAKTKEGLDH LLEMILLVSE MEELKANP

Formula weight

19500.2 Da

Source organism

Geobacillus stearothermophilus

Exptl. method

NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.3 %, Completeness: 72.2 %, Completeness (bb): 81.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.2 % (1455 of 2015)	66.7 % (692 of 1038)	78.2 % (621 of 794)	77.6 % (142 of 183)
Backbone	81.4 % (855 of 1050)	80.5 % (289 of 359)	81.4 % (425 of 522)	83.4 % (141 of 169)
Sidechain	65.2 % (737 of 1131)	59.4 % (403 of 679)	76.0 % (333 of 438)	7.1 % (1 of 14)
Aromatic	40.0 % (36 of 90)	40.0 % (18 of 45)	38.6 % (17 of 44)	100.0 % (1 of 1)
Methyl	86.0 % (203 of 236)	83.9 % (99 of 118)	88.1 % (104 of 118)

1. IF2G2

GSHMVERPPV VTIMGHVDHG KTTLLDAIRH SKVTEQEAGG ITQHIGAYQV TVNDKKITFL DTPGHEAFTT MRARGAQVTD IVILVVAADD GVMPQTVEAI NHAKAANVPI IVAINKMDKP EANPDRVMQE LMEYNLVPEE WGGDTIFCKL SAKTKEGLDH LLEMILLVSE MEELKANP

Show sample condition

Sample

Temperature 315 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	IF2G2	[U-13C; U-15N]	protein	0.6 (±0.2) mM
2	potassium phosphate		buffer	20 (±2.0) mM
3	potassiumm chloride		salt	150 (±5.0) mM
4	DTT			1 (±0.1) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.101329

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.101329

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.101329

Referencing offset: -0.12 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.101329

Referencing offset: 0.15 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LKC, Strand ID: A Detail

Release date

2006-11-06

Citation

The nucleotide-binding site of bacterial translation initiation factor 2 (IF2) as a metabolic sensor
Milon, P., Tischenko, E.V., Tomsic, J., Caserta, E., Folkers, G., La Teana, A., Rodnina, M.V., Pon, C.L., Boelens, R., Gualerzi, C.O.
Proc. Natl. Acad. Sci. U. S. A. (2006), 103, 13962-13967, PubMed 16968770 , DOI 10.1073/pnas.0606384103 , Abstract

Related entities 1. g2 free, : 1 : 1 : 199 entities Detail

Interaction partners 1. g2 free, : 4 interactors Detail

More details for 4 interactors identified by 1 citations