BMRB: 6953

NMR solution of rabbit Prion Protein (91-228)

Authors

Li, J., Lin, D.H.

Assembly

Major prion protein

Entity

1. Major prion protein (polymer, Thiol state: all disulfide bound), 138 monomers, 15638.27 Da Detail

QGGTHNQWGK PSKPKTSMKH VAGAAAAGAV VGGLGGYMLG SAMSRPLIHF GNDYEDRYYR ENMYRYPNQV YYRPVDQYSN QNSFVHDCVN ITVKQHTVTT TTKGENFTET DIKIMERVVE QMCITQYQQE SQAAYQRA

Formula weight

15638.27 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS88:SG	1:CYS123:SG

Source organism

Oryctolagus cuniculus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.5 %, Completeness (bb): 98.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.5 % (1472 of 1591)	95.2 % (788 of 828)	87.5 % (533 of 609)	98.1 % (151 of 154)
Backbone	98.5 % (806 of 818)	97.5 % (276 of 283)	99.0 % (398 of 402)	99.2 % (132 of 133)
Sidechain	88.1 % (792 of 899)	93.9 % (512 of 545)	78.4 % (261 of 333)	90.5 % (19 of 21)
Aromatic	40.7 % (61 of 150)	74.7 % (56 of 75)	5.4 % (4 of 74)	100.0 % (1 of 1)
Methyl	97.5 % (115 of 118)	98.3 % (58 of 59)	96.6 % (57 of 59)

1. Major prion protein

QGGTHNQWGK PSKPKTSMKH VAGAAAAGAV VGGLGGYMLG SAMSRPLIHF GNDYEDRYYR ENMYRYPNQV YYRPVDQYSN QNSFVHDCVN ITVKQHTVTT TTKGENFTET DIKIMERVVE QMCITQYQQE SQAAYQRA

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 4.5

#	Name	Isotope labeling	Type	Concentration
1	Major prion protein	[U-15N; U-13C]		1 mM
2	acetate buffer			20 mM
3	H2O			90 %
4	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.2 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.2 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.16 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 15 models in PDB: 2FJ3, Strand ID: A Detail

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Release date

2007-09-23

Citation

1H, 13C and 15N resonance assignments of rabbit prion protein (91-228)
Li, J., Mei, F.H., Xiao, G.F., Lin, D.H.
J. Biomol. NMR (2007), 38, 181-181, PubMed 17415669 , DOI 10.1007/s10858-006-9115-9 , Abstract

Related entities 1. Major prion protein, : 1 : 1 : 2 : 111 entities Detail

Interaction partners 1. Major prion protein, : 1 interactors Detail

Experiments performed 4 experiments Detail