BMRB: 6957

Solution structure of the SCAN homodimer from MZF-1/ZNF42

Authors

Volkman, B.F., Peterson, F.C., Sander, T.L., Waltner, J.K.

Assembly

SCAN

Entity

1. SCAN (polymer, Thiol state: all free), 94 monomers, 10504.78 × 2 Da Detail

GSDPGPEAAR LRFRCFHYEE ATGPQEALAQ LRELCRQWLR PEVRSKEQML ELLVLEQFLG ALPPEIQARV QGQRPGSPEE AAALVDGLRR EPGG

Total weight

21009.56 Da

Max. entity weight

10504.78 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

AUTOMATED METHODS WERE USED FOR BACKBONE CHEMICAL SHIFT ASSIGNMENT AND ITERATIVE NOE REFINEMENT.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 96.2 %, Completeness (bb): 97.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.2 % (1048 of 1089)	96.5 % (559 of 579)	95.9 % (399 of 416)	95.7 % (90 of 94)
Backbone	97.1 % (530 of 546)	96.8 % (182 of 188)	97.4 % (266 of 273)	96.5 % (82 of 85)
Sidechain	95.9 % (602 of 628)	96.4 % (377 of 391)	95.2 % (217 of 228)	88.9 % (8 of 9)
Aromatic	81.5 % (44 of 54)	96.3 % (26 of 27)	65.4 % (17 of 26)	100.0 % (1 of 1)
Methyl	97.9 % (92 of 94)	97.9 % (46 of 47)	97.9 % (46 of 47)

1. SCAN

GSDPGPEAAR LRFRCFHYEE ATGPQEALAQ LRELCRQWLR PEVRSKEQML ELLVLEQFLG ALPPEIQARV QGQRPGSPEE AAALVDGLRR EPGG

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	SCAN	[U-15N; U-13C]		2 mM
2	sodium phosphate			20 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.54 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.54 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.31 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2FI2, Strand ID: A, B Detail

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Release date

2006-03-28

Citation

Structure of the SCAN domain from the tumor suppressor protein MZF1
Peterson, F.C., Hayes, P.L., Waltner, J.K., Heisner, A.K., Jensen, D.R., Sander, T.L., Volkman, B.F.
J. Mol. Biol. (2006), 363, 137-147, PubMed 16950398 , DOI 10.1016/j.jmb.2006.07.063 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. SCAN, : 1 : 2 : 199 entities Detail

Interaction partners 1. SCAN, : 2 interactors Detail

Experiments performed 4 experiments Detail