BMRB: 6969

Chemical Shift Assignment for SARS-CoV S2-HR2

Authors

Hakansson-McReynolds, S., Caffrey, M., Jiang, S.

Assembly

S2-HR2 trimer

Entity

1. S2-HR2 trimer (polymer, Thiol state: not present), 55 monomers, 6084.666 × 3 Da Detail

GSHTSPDVDL GDISGINASV VNIQKEIDRL NEVAKNLNES LIDLQELGKY EQYIK

Total weight

18253.998 Da

Max. entity weight

6084.666 Da

Source organism

Severe acute respiratory syndrome-related coronavirus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 86.3 %, Completeness (bb): 94.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.3 % (544 of 630)	82.6 % (271 of 328)	89.2 % (214 of 240)	95.2 % (59 of 62)
Backbone	94.2 % (309 of 328)	93.8 % (106 of 113)	94.4 % (152 of 161)	94.4 % (51 of 54)
Sidechain	80.5 % (284 of 353)	76.7 % (165 of 215)	85.4 % (111 of 130)	100.0 % (8 of 8)
Aromatic	5.0 % (1 of 20)	0.0 % (0 of 10)	10.0 % (1 of 10)
Methyl	88.6 % (62 of 70)	88.6 % (31 of 35)	88.6 % (31 of 35)

1. S2-Hr2

GSHTSPDVDL GDISGINASV VNIQKEIDRL NEVAKNLNES LIDLQELGKY EQYIK

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	S2-Hr2	[U-95% 13C; U-95% 15N]	protein	1 (±0.1) mM
2	TFE		solvent	30 %

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Protein Blocks Logo

Calculated from 1 models in PDB: 2FXP, Strand ID: A, B, C Detail

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Release date

2006-04-26

Citation

Solution structure of the severe acute respiratory syndrome-coronavirus heptad repeat 2 domain in the prefusion state
Hakansson-McReynolds, S., Jiang, S., Rong, L., Caffrey, M.
J. Biol. Chem. (2006), 281, 11965-11971, PubMed 16507566 , DOI 10.1074/jbc.M601174200 , Abstract

Related entities 1. S2-HR2 trimer, : 1 : 146 entities Detail

Interaction partners 1. S2-HR2 trimer, : 1 interactors Detail

Experiments performed 8 experiments Detail