BMRB: 6975

1H chemical shift assignments for Bcl2MidG4

Authors

Dai, J., Chen, D., Jones, R.A., Hurley, L., Danzhou, Y.

Assembly

Bcl2MidG4Pu23-G15T G16T

Entity

1. Bcl2MidG4Pu23-G15T G16T (polymer, Thiol state: not present), 23 monomers, 7371.652 Da Detail

GGGCGCGGGA GGAATTGGGC GGG

Formula weight

7371.652 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.7 %, Completeness (bb): 95.7 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	90.7 % (195 of 215)	90.7 % (195 of 215)
Suger, PO4	95.7 % (154 of 161)	95.7 % (154 of 161)
Nucleobase	75.9 % (41 of 54)	75.9 % (41 of 54)
Aromatic	84.8 % (39 of 46)	84.8 % (39 of 46)
Methyl	100.0 % (2 of 2)	100.0 % (2 of 2)

1. Bcl2 middle G4 oligonucleotides

GGGCGCGGGA GGAATTGGGC GGG

Show sample condition

Sample

Temperature 298 (±0.01) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	Bcl2 middle G4 oligonucleotides			1.5 mM

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Release date

2006-04-26

Citation

An intramolecular G-quadruplex structure with mixed parallel/antiparallel G-strands formed in the human BCL-2 promoter region in solution
Dai, J., Dexheimer, T.S., Chen, D., Carver, M., Ambrus, A., Jones, R.A., Yang, D.
J. Am. Chem. Soc. (2006), 128, 1096-1098, PubMed , DOI: