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Found 1 Document (1.919 seconds)

BMRB: 6976

2FJ6

Solution NMR structure of the UPF0346 protein yozE from Bacillus subtilis. Northeast Structural Genomics target SR391.

Authors

Rossi, P.

Assembly

Hypothetical UPF0346 protein yozE

Entity

1. Hypothetical UPF0346 protein yozE (polymer, Thiol state: not present), 82 monomers, 9976.709 Da Detail

MKSFYHYLLK YRHPKPKDSI SEFANQAYED HSFPKTSTDY HEISSYLELN ADYLHTMATF DEAWDQYESE VHGRLEHHHH HH

Formula weight

9976.709 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

Noesy assignments using AutoStructure, dyana and xplor for simulated annealing with a total of 1096 NOE distances. An average of 16.6 constraints/restrained res, 3.7 of which long range. 208 dihedral angle constraints and 42 h-bond constraints.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 90.9 %, Completeness (bb): 93.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.9 % (901 of 991)	90.6 % (464 of 512)	90.9 % (359 of 395)	92.9 % (78 of 84)
Backbone	93.8 % (456 of 486)	92.6 % (150 of 162)	95.1 % (233 of 245)	92.4 % (73 of 79)
Sidechain	89.6 % (525 of 586)	89.7 % (314 of 350)	89.2 % (206 of 231)	100.0 % (5 of 5)
Aromatic	70.7 % (116 of 164)	70.7 % (58 of 82)	70.4 % (57 of 81)	100.0 % (1 of 1)
Methyl	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (27 of 27)

1. Hypothetical UPF0346 protein yozE

MKSFYHYLLK YRHPKPKDSI SEFANQAYED HSFPKTSTDY HEISSYLELN ADYLHTMATF DEAWDQYESE VHGRLEHHHH HH

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0346 protein yozE	[U-13C; U-15N]	1.5 mM
2	CaCl2		5 mM
3	NaCl		100 mM
4	NH4OAc		20 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.19 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.19 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2FJ6, Strand ID: A Detail

Release date

2006-04-05

Citation

Solution NMR structure of the UPF0346 protein yozE from Bacillus subtilis. Northeast Structural Genomics target SR391
Rossi, P., Acton, T.B., Cunningham, K.L., Ma, L.C., Shetty, K., Swapna, G.V.T., Xiao, R., Montelione, G.T.

Related entities 1. Hypothetical UPF0346 protein yozE, : 1 : 1 : 45 entities Detail

Experiments performed 11 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0346 protein yozE	[U-13C; U-15N]	1.5 mM
2	CaCl2		5 mM
3	NaCl		100 mM
4	NH4OAc		20 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0346 protein yozE	[U-5% 13C; U-15N]	1.35 mM
10	CaCl2		5 mM
11	NaCl		100 mM
12	NH4OAc		20 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

2 3D 13C-separated NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0346 protein yozE	[U-13C; U-15N]	1.5 mM
2	CaCl2		5 mM
3	NaCl		100 mM
4	NH4OAc		20 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0346 protein yozE	[U-5% 13C; U-15N]	1.35 mM
10	CaCl2		5 mM
11	NaCl		100 mM
12	NH4OAc		20 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

3 HNCA-J

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0346 protein yozE	[U-13C; U-15N]	1.5 mM
2	CaCl2		5 mM
3	NaCl		100 mM
4	NH4OAc		20 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0346 protein yozE	[U-5% 13C; U-15N]	1.35 mM
10	CaCl2		5 mM
11	NaCl		100 mM
12	NH4OAc		20 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

4 HiRes-CH HSQC for stereospec. VL Methyl assign.

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0346 protein yozE	[U-13C; U-15N]	1.5 mM
2	CaCl2		5 mM
3	NaCl		100 mM
4	NH4OAc		20 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0346 protein yozE	[U-5% 13C; U-15N]	1.35 mM
10	CaCl2		5 mM
11	NaCl		100 mM
12	NH4OAc		20 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

5 HNCACB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0346 protein yozE	[U-13C; U-15N]	1.5 mM
2	CaCl2		5 mM
3	NaCl		100 mM
4	NH4OAc		20 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0346 protein yozE	[U-5% 13C; U-15N]	1.35 mM
10	CaCl2		5 mM
11	NaCl		100 mM
12	NH4OAc		20 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

6 HNcoCACB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0346 protein yozE	[U-13C; U-15N]	1.5 mM
2	CaCl2		5 mM
3	NaCl		100 mM
4	NH4OAc		20 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0346 protein yozE	[U-5% 13C; U-15N]	1.35 mM
10	CaCl2		5 mM
11	NaCl		100 mM
12	NH4OAc		20 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

7 HNCO

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0346 protein yozE	[U-13C; U-15N]	1.5 mM
2	CaCl2		5 mM
3	NaCl		100 mM
4	NH4OAc		20 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0346 protein yozE	[U-5% 13C; U-15N]	1.35 mM
10	CaCl2		5 mM
11	NaCl		100 mM
12	NH4OAc		20 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

8 HNCA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0346 protein yozE	[U-13C; U-15N]	1.5 mM
2	CaCl2		5 mM
3	NaCl		100 mM
4	NH4OAc		20 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0346 protein yozE	[U-5% 13C; U-15N]	1.35 mM
10	CaCl2		5 mM
11	NaCl		100 mM
12	NH4OAc		20 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

9 CccoNH tocsy

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0346 protein yozE	[U-13C; U-15N]	1.5 mM
2	CaCl2		5 mM
3	NaCl		100 mM
4	NH4OAc		20 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0346 protein yozE	[U-5% 13C; U-15N]	1.35 mM
10	CaCl2		5 mM
11	NaCl		100 mM
12	NH4OAc		20 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

10 HCCH-COSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0346 protein yozE	[U-13C; U-15N]	1.5 mM
2	CaCl2		5 mM
3	NaCl		100 mM
4	NH4OAc		20 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0346 protein yozE	[U-5% 13C; U-15N]	1.35 mM
10	CaCl2		5 mM
11	NaCl		100 mM
12	NH4OAc		20 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

11 HAcacoNH single transfer

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0346 protein yozE	[U-13C; U-15N]	1.5 mM
2	CaCl2		5 mM
3	NaCl		100 mM
4	NH4OAc		20 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0346 protein yozE	[U-5% 13C; U-15N]	1.35 mM
10	CaCl2		5 mM
11	NaCl		100 mM
12	NH4OAc		20 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

nullKeywords AutoStructure, NESG, NMR Structure, Northeast Structural Genomics, Northeast Structural Genomics Consortium, Protein Structure Initiative, PSI, SR391

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Found 1 Document (1.919 seconds)

BMRB: 6976

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Hypothetical UPF0346 protein yozE, : 1 : 1 : 45 entities Detail

Experiments performed 11 experiments Detail

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