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Found 1 Document (1.92 seconds)

BMRB: 6979


6979
2DD3
An Alternating Sheared AA Pair and Elements of Stability for a Single Sheared Purine-Purine Pair Flanked by Sheared GA Pairs
Authors
Chen, G., Kennedy, S.D., Krugh, T.R., Turner, D.H.
Assembly
5'-R(*GP*GP*UP*GP*AP*AP*GP*GP*CP*U)-3'/5'-R(*GP*CP*CP*GP*AP*AP*GP*CP*CP*(PR5))-3'
Entity
1. GGUGAAGGCU (polymer, Thiol state: not present), 10 monomers, 3319.968 Da Detail

GGUGAAGGCU


2. GCCGAAGCCP (polymer, Thiol state: not present), 10 monomers, 3246.962 Da Detail

GCCGAAGCCX


Total weight
6566.9297 Da
Max. entity weight
3319.968 Da
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 50.0 %, Completeness: 49.2 %, Completeness (bb): 50.4 % Detail
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Polymer type: polyribonucleotide

Total1H31P
All49.2 % (90 of 183)49.4 % (81 of 164)47.4 % (9 of 19)
Suger, PO450.4 % (67 of 133)50.9 % (58 of 114)47.4 % (9 of 19)
Nucleobase46.0 % (23 of 50)46.0 % (23 of 50)
Aromatic52.5 % (21 of 40)52.5 % (21 of 40)

1. GGUGAAGGCU

GGUGAAGGCU

2. GCCGAAGCCP

GCCGAAGCCX

Show sample condition
Sample

Temperature 303 K, pH 5.1


#NameIsotope labelingTypeConcentration
1GGUGAAGGCU2 mM
2GCCGAAGCCP2 mM
3NaCl80 mM
4sodium phosphate10 mM
5Na2EDTA0.5 mM

Chem. Shift Complete2
Sequence coverage: 45.0 %, Completeness: 47.5 %, Completeness (bb): 47.4 % Detail
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Polymer type: polyribonucleotide

Total1H31P
All47.5 % (174 of 366)47.9 % (157 of 328)44.7 % (17 of 38)
Suger, PO447.4 % (126 of 266)47.8 % (109 of 228)44.7 % (17 of 38)
Nucleobase48.0 % (48 of 100)48.0 % (48 of 100)
Aromatic47.5 % (38 of 80)47.5 % (38 of 80)

1. GGUGAAGGCU

GGUGAAGGCU

2. GCCGAAGCCP

GCCGAAGCCX

Show sample condition
Sample

Temperature 303 K, pH 5.1


#NameIsotope labelingTypeConcentration
1GGUGAAGGCU2 mM
2GCCGAAGCCP2 mM
3NaCl80 mM
4sodium phosphate10 mM
5Na2EDTA0.5 mM

Release date
2006-06-25
Citation
An alternating sheared AA pair and elements of stability for a single sheared purine-purine pair flanked by sheared GA pairs in RNA
Chen, G., Kennedy, S.D., Qiao, J., Krugh, T.R., Turner, D.H.
Biochemistry (2006), 45, 6889-6903, PubMed 16734425 , DOI 10.1021/bi0524464 ,
Experiments performed 1 experiments Detail
nullKeywords alternating sheared AA pair, Kink-turn motif, rapid conformation exchange, RNA secondary structure prediction, sequence specific A-minor motif, thermodynamics