BMRB: 6981

Solution Structure of Monomeric BsaL, the Type III Secretion Needle Protein of Burkholderia pseudomallei

Authors

Zhang, L., Wang, Y., Picking, W.L., Picking, W.D., De Guzman, R.N.

Assembly

BsaL

Entity

1. BsaL (polymer, Thiol state: not present), 92 monomers, 10355.35 Da Detail

MSNPPTPLLA DYEWSGYLTG IGRAFDDGVK DLNKQLQDAQ ANLTKNPSDP TALANYQMIM SEYNLYRNAQ SSAVKSMKDI DSSILEHHHH HH

Formula weight

10355.35 Da

Source organism

Burkholderia pseudomallei

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 75.1 %, Completeness (bb): 87.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	75.1 % (791 of 1053)	75.5 % (413 of 547)	71.7 % (291 of 406)	87.0 % (87 of 100)
Backbone	87.1 % (472 of 542)	88.5 % (162 of 183)	86.0 % (234 of 272)	87.4 % (76 of 87)
Sidechain	66.1 % (396 of 599)	69.0 % (251 of 364)	60.4 % (134 of 222)	84.6 % (11 of 13)
Aromatic	11.6 % (10 of 86)	14.0 % (6 of 43)	7.1 % (3 of 42)	100.0 % (1 of 1)
Methyl	64.3 % (54 of 84)	69.0 % (29 of 42)	59.5 % (25 of 42)

1. BsaL

MSNPPTPLLA DYEWSGYLTG IGRAFDDGVK DLNKQLQDAQ ANLTKNPSDP TALANYQMIM SEYNLYRNAQ SSAVKSMKDI DSSILEHHHH HH

Show sample condition

Sample

pH 7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BsaL	[U-15N; U-13C]	protein	0.8 (±0.2) mM

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.22 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2G0U, Strand ID: A Detail

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Release date

2007-02-06

Citation

Solution structure of monomeric BsaL, the type III secretion needle protein of Burkholderia pseudomallei
Zhang, L., Wang, Y., Picking, W.L., Picking, W.D., De Guzman, R.N.
J. Mol. Biol. (2006), 359, 322-330, PubMed 16631790 , DOI 10.1016/j.jmb.2006.03.028 , Abstract

Related entities 1. BsaL, : 1 : 43 entities Detail