BMRB: 6986

NMR ASSIGNMENT OF THE PHOSPHOTYROSINE BINDING(PTB) DOMAIN OF TENSIN

Authors

Leone, M., Pellecchia, M.

Assembly

Phosphotyrosine binding domain of chicken tensin 1

Entity

1. Phosphotyrosine binding domain of chicken tensin 1 (polymer, Thiol state: all free), 144 monomers, 15650.66 Da Detail

GSHMGAACNV LFINSVEMES LTGPQAISKA VAETLVADPT PTATIVHFKV SAQGITLTDN QRKLFFRRHY PLNTVTFCDL DPQERKWTKT DGSGPAKLFG FVARKQGSTT DNVCHLFAEL DPDQPAAAIV NFVSRVMLGS GQKR

Formula weight

15650.66 Da

Source organism

Gallus gallus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 78.7 %, Completeness (bb): 92.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	78.7 % (1285 of 1632)	88.0 % (740 of 841)	63.8 % (409 of 641)	90.7 % (136 of 150)
Backbone	92.8 % (787 of 848)	93.1 % (270 of 290)	92.4 % (390 of 422)	93.4 % (127 of 136)
Sidechain	68.1 % (625 of 918)	85.3 % (470 of 551)	41.4 % (146 of 353)	64.3 % (9 of 14)
Aromatic	35.7 % (45 of 126)	69.8 % (44 of 63)	0.0 % (0 of 62)	100.0 % (1 of 1)
Methyl	61.4 % (102 of 166)	96.4 % (80 of 83)	26.5 % (22 of 83)

1. Phosphotyrosine Binding Domain of tensin

GSHMGAACNV LFINSVEMES LTGPQAISKA VAETLVADPT PTATIVHFKV SAQGITLTDN QRKLFFRRHY PLNTVTFCDL DPQERKWTKT DGSGPAKLFG FVARKQGSTT DNVCHLFAEL DPDQPAAAIV NFVSRVMLGS GQKR

Show sample condition

Sample #1

Temperature 298.0 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Phosphotyrosine Binding Domain of tensin	[U- 13C; U- 15N]	protein	1 mM
2	potassium phosphate		buffer	50 mM
3	NaCl		salt	150 mM

Sample #2

Temperature 298.0 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Phosphotyrosine Binding Domain of tensin	[U-15N]	protein	1 mM
5	Potassium phosphate		buffer	50 mM
6	NaCl		salt	150 mM

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LACS Plot; CA

Referencing offset: 2.5 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.5 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.99 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2GJY, Strand ID: A Detail

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Release date

2006-06-27

Citation

NMR assignment of the phosphotyrosine binding (PTB) domain of tensin
Leone, M., Yu, E.C.W., Liddington, R., Pellecchia, M.
J. Biomol. NMR (2006), 36 Suppl 1, 40-40, PubMed 16705357 , DOI 10.1007/s10858-006-9011-3 , Abstract

Related entities 1. Phosphotyrosine binding domain of chicken tensin 1, : 1 : 2 : 32 entities Detail

Interaction partners 1. Phosphotyrosine binding domain of chicken tensin 1, : 3 interactors Detail

Experiments performed 9 experiments Detail