BMRB: 6989

1H, 13C, and 15N Chemical Shift Assignments for Ribosomal Protein S24E

Authors

Jeon, B., Hong, E., Jung, J., Yee, A., Cheryl H, A., Lee, W.

Assembly

Ribosomal Protein S24E

Entity

1. Ribosomal Protein S24E (polymer, Thiol state: all free), 98 monomers, 11516.17 Da Detail

MDLIIKEKRD NPILKRKEIK YVLKFDSSRT PSREEIKELI AKHEGVDKEL VIVDNNKQLT GKHEIEGYTK IYADKPSAML YEPDYELIRN GLKQKEAK

Formula weight

11516.17 Da

Source organism

Thermoplasma acidophilum

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 83.3 %, Completeness (bb): 96.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.3 % (1020 of 1224)	79.0 % (516 of 653)	87.3 % (411 of 471)	93.0 % (93 of 100)
Backbone	96.0 % (557 of 580)	92.9 % (182 of 196)	97.6 % (283 of 290)	97.9 % (92 of 94)
Sidechain	75.3 % (556 of 738)	73.1 % (334 of 457)	80.4 % (221 of 275)	16.7 % (1 of 6)
Aromatic	17.2 % (10 of 58)	34.5 % (10 of 29)	0.0 % (0 of 29)
Methyl	92.5 % (98 of 106)	94.3 % (50 of 53)	90.6 % (48 of 53)

1. Ribosomal Protein S24E

MDLIIKEKRD NPILKRKEIK YVLKFDSSRT PSREEIKELI AKHEGVDKEL VIVDNNKQLT GKHEIEGYTK IYADKPSAML YEPDYELIRN GLKQKEAK

Show sample condition

Sample #1

Temperature 298 K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ribosomal Protein S24E	[U-13C; U-15N]		1.5 mM
2	photasuim phosphate			50 mM
3	NaN3			0.1 mM
4	H2O			90 %
5	D2O			10 %

Sample #2

Temperature 298 K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ribosomal Protein S24E	[U-15N]		1.5 mM
7	photasuim phosphate			50 mM
8	NaN3			0.1 mM
9	H2O			90 %
10	D2O			10 %

Sample #3

Temperature 298 K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
11	Ribosomal Protein S24E	[U-13C]		1.5 mM
12	photasuim phosphate			50 mM
13	NaN3			0.1 mM
14	D2O			100 %

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; CO

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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Protein Blocks Logo

Calculated from 10 models in PDB: 2G1D, Strand ID: A Detail

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Release date

2007-03-05

Citation

Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit
Ban, N., Nissen, P., Hansen, J., Capel, M., Moore, P., Steitz, T.
Nature (1999), 400, 841-847, PubMed 10476961 , DOI 10.1038/23641 , Abstract

Entries sharing articles Swiss-Prot: 8 entries Detail

Related entities 1. Ribosomal Protein S24E, : 1 : 1 : 178 entities Detail

Experiments performed 8 experiments Detail