BMRB: 6991

Phosphorylation of Cytoplasmic Tail of Tissue Factor and its Role in Modulating

Authors

Sen, M., Agrawal, S., Craft, J., Ruf, W., Legge, G.B.

Assembly

TFCD

Entity

1. TFCD (polymer, Thiol state: all free), 19 monomers, 2060.293 × 2 Da Detail

CRKAGVGQSW KENSPLNVS

Total weight

4120.586 Da

Max. entity weight

2060.293 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

DYANA AMBER 8.0.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.5 %, Completeness: 73.0 %, Completeness (bb): 72.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	73.0 % (157 of 215)	85.1 % (97 of 114)	51.9 % (41 of 79)	86.4 % (19 of 22)
Backbone	72.3 % (81 of 112)	89.7 % (35 of 39)	56.4 % (31 of 55)	83.3 % (15 of 18)
Sidechain	73.3 % (88 of 120)	82.7 % (62 of 75)	53.7 % (22 of 41)	100.0 % (4 of 4)
Aromatic	58.3 % (7 of 12)	100.0 % (6 of 6)	0.0 % (0 of 5)	100.0 % (1 of 1)
Methyl	85.7 % (12 of 14)	100.0 % (7 of 7)	71.4 % (5 of 7)

1. TFCD

CRKAGVGQSW KENSPLNVS

Show sample condition

Sample

Pressure 1 (±0) atm, Temperature 285 (±0.3) K, pH 6.0 (±0.05), Details 6mM TFCD in 10% D2O pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	TFCD		protein	6 (±0.05) mM
2	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 10 models in PDB: 2CEH, Strand ID: A Detail

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Release date

2006-02-14

Citation

Spectroscopic Characterization of Successive Phosphorylation of the Tissue Factor Cytoplasmic Region
Sen, M., Herzik, M., Craft, J.W., Creath, A.L., Agrawal, S., Ruf, W., Legge, G.B.
Open Spectrosc. J. (2009), 3, 58-64, PubMed 20076769 , DOI 10.2174/1874383800903010058 , Abstract

Related entities 1. TFCD, : 1 : 2 : 3 : 2 entities Detail

Interaction partners 1. TFCD, : 3 interactors Detail

Experiments performed 6 experiments Detail