BMRB: 6993

Phosphorylation of Cytoplasmic Tail of Tissue Factor and its Role in Modulating Structure and Binding Affinity.

Authors

Sen, M., Agrawal, S., Craft, J., Ruf, W., Legge, G.B.

Assembly

TFPP

Entity

1. TFPP (polymer, Thiol state: all free), 19 monomers, 2220.251 × 2 Da Detail

CRKAGVGQXW KENXPLNVS

Total weight

4440.502 Da

Max. entity weight

2220.251 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

DYANA AMBER 8.0

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 78.9 %, Completeness: 67.3 %, Completeness (bb): 65.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	67.3 % (134 of 199)	84.0 % (89 of 106)	39.7 % (29 of 73)	80.0 % (16 of 20)
Backbone	65.0 % (65 of 100)	88.6 % (31 of 35)	44.9 % (22 of 49)	75.0 % (12 of 16)
Sidechain	68.4 % (78 of 114)	81.7 % (58 of 71)	41.0 % (16 of 39)	100.0 % (4 of 4)
Aromatic	58.3 % (7 of 12)	100.0 % (6 of 6)	0.0 % (0 of 5)	100.0 % (1 of 1)
Methyl	57.1 % (8 of 14)	100.0 % (7 of 7)	14.3 % (1 of 7)

1. TFPP

CRKAGVGQXW KENXPLNVS

Show sample condition

Sample

Pressure 1 (±0) atm, Temperature 285 (±0.3) K, pH 6.0 (±0.05), Details 6mM TFPP in 10% D2O pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	TFPP		protein	6 (±0.05) mM
2	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 10 models in PDB: 2CEF, Strand ID: A Detail

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Release date

2006-02-16

Citation

Spectroscopic Characterization of Successive Phosphorylation of the Tissue Factor Cytoplasmic Region
Sen, M., Herzik, M., Craft, J.W., Creath, A.L., Agrawal, S., Ruf, W., Legge, G.B.
Open Spectrosc. J. (2009), 3, 58-64, PubMed 20076769 , DOI 10.2174/1874383800903010058 , Abstract

Related entities 1. TFPP, : 1 : 5 entities Detail

Interaction partners 1. TFPP, : 3 interactors Detail

Experiments performed 6 experiments Detail