BMRBj - BMREntryMaster

Found 1 Document (1.722 seconds)

BMRB: 6995

2LKD

Solution structure of GDP-bound form of the GTP/GDP binding domain of translation initiation factor IF2

Authors

Tischenko, E.V., Folkers, G., Boelens, R., Gualerzi, C.O.

Assembly

g2 complexed with GDP

Entity

1. IF2G2-GDP (polymer, Thiol state: all free), 178 monomers, 19500.20 Da Detail

GSHMVERPPV VTIMGHVDHG KTTLLDAIRH SKVTEQEAGG ITQHIGAYQV TVNDKKITFL DTPGHEAFTT MRARGAQVTD IVILVVAADD GVMPQTVEAI NHAKAANVPI IVAINKMDKP EANPDRVMQE LMEYNLVPEE WGGDTIFCKL SAKTKEGLDH LLEMILLVSE MEELKANP

2. GDP (non-polymer), 443.201 Da

Total weight

19943.4 Da

Max. entity weight

19500.2 Da

Source organism

Geobacillus stearothermophilus

Exptl. method

NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.9 %, Completeness: 77.8 %, Completeness (bb): 82.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.8 % (1568 of 2015)	74.3 % (771 of 1038)	81.5 % (647 of 794)	82.0 % (150 of 183)
Backbone	82.2 % (863 of 1050)	82.2 % (295 of 359)	81.8 % (427 of 522)	83.4 % (141 of 169)
Sidechain	74.9 % (847 of 1131)	70.1 % (476 of 679)	82.6 % (362 of 438)	64.3 % (9 of 14)
Aromatic	62.2 % (56 of 90)	62.2 % (28 of 45)	61.4 % (27 of 44)	100.0 % (1 of 1)
Methyl	85.6 % (202 of 236)	84.7 % (100 of 118)	86.4 % (102 of 118)

1. IF2G2-GDP

GSHMVERPPV VTIMGHVDHG KTTLLDAIRH SKVTEQEAGG ITQHIGAYQV TVNDKKITFL DTPGHEAFTT MRARGAQVTD IVILVVAADD GVMPQTVEAI NHAKAANVPI IVAINKMDKP EANPDRVMQE LMEYNLVPEE WGGDTIFCKL SAKTKEGLDH LLEMILLVSE MEELKANP

Show sample condition

Sample

Temperature 315 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	IF2G2	[U-13C; U-15N]	protein	0.6 (±0.2) mM
2	potassium phosphate		buffer	20 (±2.0) mM
3	potassiumm chloride		salt	150 (±5.0) mM
4	DTT		reducing agent	1 (±0.1) mM
5	GDP		additive	1 (±0.1) mM
6	MgCl2		salt	5 (±0.2) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.101329

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.101329

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.101329

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.101329

Referencing offset: 0.18 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 18 models in PDB: 2LKD, Strand ID: A Detail

Release date

2006-11-06

Citation

The nucleotide-binding site of bacterial translation initiation factor 2 (IF2) as a metabolic sensor
Milon, P., Tischenko, E.V., Tomsic, J., Caserta, E., Folkers, G., La Teana, A., Rodnina, M.V., Pon, C.L., Boelens, R., Gualerzi, C.O.
Proc. Natl. Acad. Sci. U. S. A. (2006), 103, 13962-13967, PubMed 16968770 , DOI 10.1073/pnas.0606384103 , Abstract

Related entities 1. IF2G2-GDP, : 1 : 1 : 199 entities Detail

Interaction partners 1. IF2G2-GDP, : 4 interactors Detail

More details for 4 interactors identified by 1 citations