Structural Insights of the Specificity and Catalysis of a Dimeric Viral SET Domain Histone H3 Lysine-27 Methyltransferase
MFNDRVIVKK SPLGGYGVFA RKSFEKGELV EECLCIVRHN DDWGTALEDY LFSRKNMSAM ALGFGAIFNH SKDPNARHEL TAGLKRMRIF TIKPIAIGEE ITISYGDDYW LSRPRLTQN
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 74.6 % (1214 of 1628) | 80.8 % (688 of 851) | 65.3 % (414 of 634) | 78.3 % (112 of 143) |
Backbone | 76.9 % (632 of 822) | 93.7 % (266 of 284) | 62.7 % (254 of 405) | 84.2 % (112 of 133) |
Sidechain | 74.1 % (691 of 933) | 74.4 % (422 of 567) | 75.6 % (269 of 356) | 0.0 % (0 of 10) |
Aromatic | 52.2 % (72 of 138) | 53.6 % (37 of 69) | 52.2 % (35 of 67) | 0.0 % (0 of 2) |
Methyl | 93.0 % (132 of 142) | 98.6 % (70 of 71) | 87.3 % (62 of 71) |
1. 13C/15N vSET complex
MFNDRVIVKK SPLGGYGVFA RKSFEKGELV EECLCIVRHN DDWGTALEDY LFSRKNMSAM ALGFGAIFNH SKDPNARHEL TAGLKRMRIF TIKPIAIGEE ITISYGDDYW LSRPRLTQN2. me K27 H3 Peptide
GKAPRKQLAT KAARXSAPAT GTemperature 310 K, pH 6.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | 13C/15N vSET complex | [U-13C; U-15N] | 5.0 mM |