BMRB: 6997

Structural Insights of the Specificity and Catalysis of a Dimeric Viral SET Domain Histone H3 Lysine-27 Methyltransferase

Authors

Qian, C.

Assembly

vSET complex with meK27 H3 Pept and cofactor product SAH

Entity

1. 13C/15N vSET complex (polymer, Thiol state: all free), 119 monomers, 13586.46 Da Detail

MFNDRVIVKK SPLGGYGVFA RKSFEKGELV EECLCIVRHN DDWGTALEDY LFSRKNMSAM ALGFGAIFNH SKDPNARHEL TAGLKRMRIF TIKPIAIGEE ITISYGDDYW LSRPRLTQN

2. me K27 H3 Peptide (polymer, Thiol state: all free), 21 monomers, 2488.504 Da Detail

GKAPRKQLAT KAARXSAPAT G

3. SAH (non-polymer), 384.411 Da

Total weight

16459.375 Da

Max. entity weight

13586.46 Da

Source organism

Paramecium bursaria

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 74.6 %, Completeness (bb): 76.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	74.6 % (1214 of 1628)	80.8 % (688 of 851)	65.3 % (414 of 634)	78.3 % (112 of 143)
Backbone	76.9 % (632 of 822)	93.7 % (266 of 284)	62.7 % (254 of 405)	84.2 % (112 of 133)
Sidechain	74.1 % (691 of 933)	74.4 % (422 of 567)	75.6 % (269 of 356)	0.0 % (0 of 10)
Aromatic	52.2 % (72 of 138)	53.6 % (37 of 69)	52.2 % (35 of 67)	0.0 % (0 of 2)
Methyl	93.0 % (132 of 142)	98.6 % (70 of 71)	87.3 % (62 of 71)

1. 13C/15N vSET complex

MFNDRVIVKK SPLGGYGVFA RKSFEKGELV EECLCIVRHN DDWGTALEDY LFSRKNMSAM ALGFGAIFNH SKDPNARHEL TAGLKRMRIF TIKPIAIGEE ITISYGDDYW LSRPRLTQN

2. me K27 H3 Peptide

GKAPRKQLAT KAARXSAPAT G

Show sample condition

Sample

Temperature 310 K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	13C/15N vSET complex	[U-13C; U-15N]		5.0 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 2G46, Strand ID: A, B, C, D Detail

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Release date

2007-02-06

Citation

Structural insights of the specificity and catalysis of a viral histone H3 lysine 27 methyltransferase
Qian, C., Wang, X., Manzur, K., Sachchidanand, Farooq, A., Zeng, L., Wang, R., Zhou, M.
J. Mol. Biol. (2006), 359, 86-96, PubMed 16603186 , DOI 10.1016/j.jmb.2006.03.006 , Abstract

Related entities 1. 13C/15N vSET complex, : 1 : 4 : 115 entities Detail

Related entities 2. me K27 H3 Peptide, : 57 : 74 entities Detail

Interaction partners 2. me K27 H3 Peptide, : 15 interactors Detail

Experiments performed 10 experiments Detail