BMRB: 7000

Solution structure of the conserved hypothetical protein Rv2302 from the bacterium Mycobacterium tuberculosis

Authors

Buchko, G.W., Kim, C.Y., Terwilliger, T.C., Kennedy, M.A.

Assembly

Rv2302

Entity

1. Rv2302 (polymer, Thiol state: not present), 83 monomers, 8871.662 Da Detail

GSHMHAKVGD YLVVKGTTTE RHDQHAEIIE VRSADGSPPY VVRWLVNGHE TTVYPGSDAV VVTATEHAEA EKRAAARAGH AAT

Formula weight

8871.662 Da

Source organism

Mycobacterium tuberculosis

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.8 %, Completeness: 85.4 %, Completeness (bb): 89.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.4 % (747 of 875)	83.5 % (369 of 442)	86.9 % (304 of 350)	89.2 % (74 of 83)
Backbone	89.4 % (440 of 492)	88.2 % (150 of 170)	90.5 % (219 of 242)	88.7 % (71 of 80)
Sidechain	82.4 % (378 of 459)	80.5 % (219 of 272)	84.8 % (156 of 184)	100.0 % (3 of 3)
Aromatic	57.8 % (37 of 64)	59.4 % (19 of 32)	54.8 % (17 of 31)	100.0 % (1 of 1)
Methyl	95.1 % (97 of 102)	94.1 % (48 of 51)	96.1 % (49 of 51)

1. Rv2302

GSHMHAKVGD YLVVKGTTTE RHDQHAEIIE VRSADGSPPY VVRWLVNGHE TTVYPGSDAV VVTATEHAEA EKRAAARAGH AAT

Show sample condition

Sample

Temperature 298 (±1) K, pH 7.1 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Rv2302	[U-13C; U-15N]	protein	2 (±0.3) mM
2	KCl	none	salt	100 (±2.0) mM
3	potassium phosphate	none	salt	20 (±1.0) mM
4	dithiothreitol	none	salt	2 (±0.5) mM

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LACS Plot; CA

Referencing offset: -0.26 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.26 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.18 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 25 models in PDB: 2A7Y, Strand ID: A Detail

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Release date

2006-03-19

Citation

Solution structure of the conserved hypothetical protein Rv2302 from Mycobacterium tuberculosis
Buchko, G.W., Kim, C.Y., Terwilliger, T.C., Kennedy, M.A.
J. Bacteriol. (2006), 188, 5993-6001, PubMed 16885468 , DOI 10.1128/JB.00460-06 , Abstract

Related entities 1. Rv2302, : 1 : 2 : 4 entities Detail

Experiments performed 10 experiments Detail