BMRB: 7002

1H, 13C, and 15N Chemical Shift Assignments for the Ede1 UBA-ubiquitin complex

Authors

Swanson, K.A., Radhakrishnan, I.

Assembly

Ede1 UBA-ubiquitin complex

Entity

1. Ubiquitin (polymer, Thiol state: all free), 76 monomers, 8556.668 Da Detail

MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

2. Ede1 UBA (polymer, Thiol state: all free), 43 monomers, 4684.082 Da Detail

TTPKSLAVEE LSGMGFTEEE AHNALEKCNW DLEAATNFLL DSA

Total weight

13240.75 Da

Max. entity weight

8556.668 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

Torsion angle dynamics and simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.8 %, Completeness (bb): 96.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.8 % (1260 of 1373)	90.2 % (643 of 713)	92.5 % (492 of 532)	97.7 % (125 of 128)
Backbone	96.0 % (680 of 708)	94.7 % (230 of 243)	96.6 % (337 of 349)	97.4 % (113 of 116)
Sidechain	88.8 % (689 of 776)	87.9 % (413 of 470)	89.8 % (264 of 294)	100.0 % (12 of 12)
Aromatic	51.5 % (35 of 68)	73.5 % (25 of 34)	27.3 % (9 of 33)	100.0 % (1 of 1)
Methyl	95.8 % (138 of 144)	95.8 % (69 of 72)	95.8 % (69 of 72)

1. Ubiquitin

MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

2. Ede1 UBA

TTPKSLAVEE LSGMGFTEEE AHNALEKCNW DLEAATNFLL DSA

Show sample condition

Sample

pH 6.0 (±0), Details 1mM each Ede1 UBA and ubiquitin

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin			1 mM
2	Ede1 UBA			1 mM
3	Sodium Phosphate			20 mM

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Calculated from 20 models in PDB: 2G3Q, Strand ID: A, B Detail

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Release date

2006-04-26

Citation

Structural basis for monoubiquitin recognition by the Ede1 UBA domain
Swanson, K.A., Hicke, L., Radhakrishnan, I.
J. Mol. Biol. (2006), 358, 713-724, PubMed 16563434 , DOI 10.1016/j.jmb.2006.02.059 , Abstract

Related entities 1. Ubiquitin, : 21 : 119 : 182 entities Detail

Related entities 2. Ede1 UBA, : 1 : 2 : 94 entities Detail

Interaction partners 2. Ede1 UBA, : 26 interactors Detail