BMRB: 7008

Second Ca2+ binding domain of the Na,Ca-exchanger (NCX1)

Authors

Hilge, M., Vuister, G.W., Aelen, J.

Assembly

Sodium/calcium exchanger 1

Entity

1. Sodium/calcium exchanger 1 (polymer, Thiol state: not present), 165 monomers, 18837.74 Da Detail

MSHHHHHHHA GIFTFEEPVT HVSESIGIME VKVLRTSGAR GNVIVPYKTI EGTARGGGED FEDTCGELEF QNDEIVKTIS VKVIDDEEYE KNKTFFLEIG EPRLVEMSEK KGGFTITEEY DDKQPLTSKE EEERRIAEMG RPILGEHTKL EVIIEESYEF KSTVD

2. CA (non-polymer), 40.078 Da

Total weight

18877.818 Da

Max. entity weight

18837.74 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.2 %, Completeness: 81.5 %, Completeness (bb): 78.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	81.5 % (1561 of 1916)	89.7 % (894 of 997)	68.4 % (516 of 754)	91.5 % (151 of 165)
Backbone	78.8 % (772 of 980)	93.8 % (318 of 339)	63.6 % (306 of 481)	92.5 % (148 of 160)
Sidechain	85.2 % (926 of 1087)	87.5 % (576 of 658)	81.8 % (347 of 424)	60.0 % (3 of 5)
Aromatic	54.1 % (80 of 148)	67.6 % (50 of 74)	40.5 % (30 of 74)
Methyl	97.6 % (162 of 166)	98.8 % (82 of 83)	96.4 % (80 of 83)

1. Sodium/calcium exchanger 1

MSHHHHHHHA GIFTFEEPVT HVSESIGIME VKVLRTSGAR GNVIVPYKTI EGTARGGGED FEDTCGELEF QNDEIVKTIS VKVIDDEEYE KNKTFFLEIG EPRLVEMSEK KGGFTITEEY DDKQPLTSKE EEERRIAEMG RPILGEHTKL EVIIEESYEF KSTVD

Show sample condition

Sample

Pressure 1 atm, Temperature 306 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	Sodium/calcium exchanger 1	[U-13C; U-15N]		0.5 ~ 1.0 mM
2	HEPES			20 mM
3	beta-Mercaptoethanol buffer			20 mM
4	H2O			95 %
5	D2O			5 %

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 6 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 6 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 2FWU, Strand ID: A Detail

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Release date

2006-04-10

Citation

Ca2+ regulation in the Na+/Ca2+ exchanger involves two markedly different Ca2+ sensors
Hilge, M., Aelen, J., Vuister, G.W.
Mol. Cell (2006), 22, 15-25, PubMed 16600866 , DOI 10.1016/j.molcel.2006.03.008 , Abstract

Related entities 1. Sodium/calcium exchanger 1, : 1 : 2 : 39 entities Detail

Interaction partners 1. Sodium/calcium exchanger 1, : 2 interactors Detail

Experiments performed 3 experiments Detail