BMRB: 7010

Solution Structure and Immune Response to an Epidermal Growth Factor Domain from Plasmodium Falciparum Merozoite Surface Protein 1

Authors

James, S., Moehle, K., Pluschke, G., Robinson, J.

Assembly

Merozoite surface protein 1

Entity

1. Merozoite surface protein 1 (polymer, Thiol state: all disulfide bound), 49 monomers, 5591.235 Da Detail

NISQHQCVKK QCPQNSGCFR HLDEREECKC LLNYKQEGDK CVENPNPTX

Formula weight

5591.235 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS18:SG
2	disulfide	sing	1:CYS12:SG	1:CYS28:SG
3	disulfide	sing	1:CYS30:SG	1:CYS41:SG

Source organism

Plasmodium falciparum

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 98.0 %, Completeness: 96.7 %, Completeness (bb): 91.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	96.7 % (296 of 306)	96.7 % (296 of 306)
Backbone	91.6 % (87 of 95)	91.6 % (87 of 95)
Sidechain	99.1 % (209 of 211)	99.1 % (209 of 211)
Aromatic	92.3 % (12 of 13)	92.3 % (12 of 13)
Methyl	100.0 % (13 of 13)	100.0 % (13 of 13)

1. Merozoite surface protein 1

NISQHQCVKK QCPQNSGCFR HLDEREECKC LLNYKQEGDK CVENPNPTX

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5

#	Name	Isotope labeling	Type	Concentration
1	Merozoite surface protein 1			1.25 mM
2	H2O			90 %
3	D2O			10 %

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Chem. Shift Complete2

Sequence coverage: 98.0 %, Completeness: 89.1 %, Completeness (bb): 77.9 % Detail

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• BMRB/XML (partial)
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Polymer type: polypeptide(L)

	Total	1H
All	89.1 % (545 of 612)	89.1 % (545 of 612)
Backbone	77.9 % (148 of 190)	77.9 % (148 of 190)
Sidechain	94.1 % (397 of 422)	94.1 % (397 of 422)
Aromatic	92.3 % (24 of 26)	92.3 % (24 of 26)
Methyl	92.3 % (24 of 26)	92.3 % (24 of 26)

1. Merozoite surface protein 1

NISQHQCVKK QCPQNSGCFR HLDEREECKC LLNYKQEGDK CVENPNPTX

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5

#	Name	Isotope labeling	Type	Concentration
4	Merozoite surface protein 1			1.25 mM
5	D2O			100 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2FLG, Strand ID: A Detail

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Release date

2006-02-27

Citation

Synthesis, solution structure and immune recognition of an epidermal growth factor-like domain from Plasmodium falciparum merozoite surface protein-1
James, S., Moehle, K., Renard, A., Mueller, M.S., Vogel, D., Zurbriggen, R., Pluschke, G., Robinson, J.A.
Chembiochem. (2006), 7, 1943-1950, PubMed 17068840 , DOI 10.1002/cbic.200600357 , Abstract

Related entities 1. Merozoite surface protein 1, : 1 : 5 : 23 entities Detail

Experiments performed 3 experiments Detail