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BMRB: 7014

2GDT

NMR assigment of the SARS-CoV protein nsp1

Authors

Almeida, M.S., Geralt, M., Johnson, M.A., Saikatendu, K., Joseph, J., Subramanian, V., Stevens, R.C., Kuhn, P., Wuthrich, K.

Assembly

nsp1

Entity

1. nsp1 (polymer, Thiol state: all free), 116 monomers, 12663.38 Da Detail

GHVQLSLPVL QVRDVLVRGF GDSVEEALSE AREHLKNGTC GLVELEKGVL PQLEQPYVFI KRSDALSTNH GHKVVELVAE MDGIQYGRSG ITLGVLVPHV GETPIAYRNV LLRKNG

Formula weight

12663.38 Da

Source organism

Severe acute respiratory syndrome-related coronavirus

Exptl. method

Refine. method

ATNOS/CANDID/CYANA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.6 %, Completeness (bb): 97.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.6 % (1292 of 1324)	97.8 % (670 of 685)	97.5 % (506 of 519)	96.7 % (116 of 120)
Backbone	97.7 % (670 of 686)	98.3 % (236 of 240)	97.6 % (327 of 335)	96.4 % (107 of 111)
Sidechain	97.8 % (725 of 741)	97.5 % (434 of 445)	98.3 % (282 of 287)	100.0 % (9 of 9)
Aromatic	84.4 % (54 of 64)	84.4 % (27 of 32)	84.4 % (27 of 32)
Methyl	100.0 % (162 of 162)	100.0 % (81 of 81)	100.0 % (81 of 81)

1. leader protein from SARS-CoV

GHVQLSLPVL QVRDVLVRGF GDSVEEALSE AREHLKNGTC GLVELEKGVL PQLEQPYVFI KRSDALSTNH GHKVVELVAE MDGIQYGRSG ITLGVLVPHV GETPIAYRNV LLRKNG

Show sample condition

Sample

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7 (±0.01), Details 25 mM Na Phosphate pH 7.0 250 mM NaCl 0.03% Azide 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	leader protein from SARS-CoV	[U-13C; U-15N]	protein	2 (±0.2) mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	250 mM
4	Azide			0.03 %
5	D2O			10 %

LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2GDT, Strand ID: A Detail

Release date

2006-08-08

Citation

NMR assignment of the SARS-CoV protein nsp1
Almeida, M.S., Johnson, M.A., Wuthrich, K.
J. Biomol. NMR (2006), 36 Suppl 1, 46-46, PubMed 16821128 , DOI 10.1007/s10858-006-9018-9 , Abstract

Related entities 1. nsp1, : 1 : 3 : 2 : 33 entities Detail

Experiments performed 10 experiments Detail

1 2D [15N,1H]-HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7 (±0.01), Details 25 mM Na Phosphate pH 7.0 250 mM NaCl 0.03% Azide 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	leader protein from SARS-CoV	[U-13C; U-15N]	protein	2 (±0.2) mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	250 mM
4	Azide			0.03 %
5	D2O			10 %

2 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7 (±0.01), Details 25 mM Na Phosphate pH 7.0 250 mM NaCl 0.03% Azide 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	leader protein from SARS-CoV	[U-13C; U-15N]	protein	2 (±0.2) mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	250 mM
4	Azide			0.03 %
5	D2O			10 %

3 2D [13C,1H]-HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7 (±0.01), Details 25 mM Na Phosphate pH 7.0 250 mM NaCl 0.03% Azide 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	leader protein from SARS-CoV	[U-13C; U-15N]	protein	2 (±0.2) mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	250 mM
4	Azide			0.03 %
5	D2O			10 %

4 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7 (±0.01), Details 25 mM Na Phosphate pH 7.0 250 mM NaCl 0.03% Azide 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	leader protein from SARS-CoV	[U-13C; U-15N]	protein	2 (±0.2) mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	250 mM
4	Azide			0.03 %
5	D2O			10 %

5 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7 (±0.01), Details 25 mM Na Phosphate pH 7.0 250 mM NaCl 0.03% Azide 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	leader protein from SARS-CoV	[U-13C; U-15N]	protein	2 (±0.2) mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	250 mM
4	Azide			0.03 %
5	D2O			10 %

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7 (±0.01), Details 25 mM Na Phosphate pH 7.0 250 mM NaCl 0.03% Azide 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	leader protein from SARS-CoV	[U-13C; U-15N]	protein	2 (±0.2) mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	250 mM
4	Azide			0.03 %
5	D2O			10 %

7 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7 (±0.01), Details 25 mM Na Phosphate pH 7.0 250 mM NaCl 0.03% Azide 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	leader protein from SARS-CoV	[U-13C; U-15N]	protein	2 (±0.2) mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	250 mM
4	Azide			0.03 %
5	D2O			10 %

8 3D CC(co)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7 (±0.01), Details 25 mM Na Phosphate pH 7.0 250 mM NaCl 0.03% Azide 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	leader protein from SARS-CoV	[U-13C; U-15N]	protein	2 (±0.2) mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	250 mM
4	Azide			0.03 %
5	D2O			10 %

9 3D 13C-[1H,1H] NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7 (±0.01), Details 25 mM Na Phosphate pH 7.0 250 mM NaCl 0.03% Azide 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	leader protein from SARS-CoV	[U-13C; U-15N]	protein	2 (±0.2) mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	250 mM
4	Azide			0.03 %
5	D2O			10 %

10 3D 15N-[1H,1H] NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 7 (±0.01), Details 25 mM Na Phosphate pH 7.0 250 mM NaCl 0.03% Azide 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	leader protein from SARS-CoV	[U-13C; U-15N]	protein	2 (±0.2) mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	250 mM
4	Azide			0.03 %
5	D2O			10 %

nullKeywords NMR, nsp, SARS-CoV