BMRB: 7019

NMR assignment of the protein nsp3a from SARS-CoV

Authors

Serrano, P., Almeida, M.S., Johnson, M.A., Saikatendu, K., Subramanian, V., Joseph, J., Stevens, R.C., Kuhn, P., Wuthrich, K.

Assembly

nsp3a

Entity

1. nsp3a (polymer, Thiol state: all free), 112 monomers, 12639.94 Da Detail

APIKGVTFGE DTVWEVQGYK NVRITFELDE RVDKVLNEKC SVYTVESGTE VTEFACVVAE AVVKTLQPVS DLLTNMGIDL DEWSVATFYL FDDAGEENFS SRMYCSFYPP DE

Formula weight

12639.94 Da

Source organism

Severe acute respiratory syndrome-related coronavirus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.5 %, Completeness (bb): 98.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.5 % (1236 of 1281)	95.6 % (624 of 653)	97.3 % (498 of 512)	98.3 % (114 of 116)
Backbone	98.0 % (651 of 664)	96.9 % (219 of 226)	98.8 % (326 of 330)	98.1 % (106 of 108)
Sidechain	95.6 % (691 of 723)	94.8 % (405 of 427)	96.5 % (278 of 288)	100.0 % (8 of 8)
Aromatic	91.0 % (122 of 134)	89.6 % (60 of 67)	92.3 % (60 of 65)	100.0 % (2 of 2)
Methyl	96.9 % (126 of 130)	96.9 % (63 of 65)	96.9 % (63 of 65)

1. nsp3a

APIKGVTFGE DTVWEVQGYK NVRITFELDE RVDKVLNEKC SVYTVESGTE VTEFACVVAE AVVKTLQPVS DLLTNMGIDL DEWSVATFYL FDDAGEENFS SRMYCSFYPP DE

Show sample condition

Sample

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	nsp3a	[U-13C; U-15N]		1.0 ~ 2.5 mM
2	sodium phosphate			50 mM
3	NaCl			150 mM

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LACS Plot; CA

Referencing offset: 2.66 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.66 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.82 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2GRI, Strand ID: A Detail

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Release date

2007-02-13

Citation

NMR assignment of the protein nsp3a from SARS-CoV
Serrano, P., Almeida, M.S., Johnson, M.A., Wuthrich, K.
J. Biomol. NMR (2006), 36 Suppl 1, 45-45, PubMed 16799860 , DOI 10.1007/s10858-006-9017-x , Abstract

Related entities 1. nsp3a, : 1 : 3 : 47 entities Detail

Experiments performed 7 experiments Detail