BMRBj - BMREntryMaster

Found 1 Document (1.649 seconds)

BMRB: 7034

2GHF

1H, 13C, and 15N chemical shift assignments for the zinc-finger region of human ZHX1

Authors

Wienk, H., Lammers, I., Wu, J., Hotze, A., Wechselberger, R., Kaptein, R., Folkers, G.

Assembly

hZHX1-ZNF

Entity

1. hZHX1-ZNF (polymer, Thiol state: all other bound), 102 monomers, 12114.38 Da Detail

MAHHHHHHNQ QNKKVEGGYE CKYCTFQTPD LNMFTFHVDS EHPNVVLNSS YVCVECNFLT KRYDALSEHN LKYHPGEENF KLTMVKRNNQ TIFEQTINDL TF

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

12245.198 Da

Max. entity weight

12114.38 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS21:SG	2:ZN1:ZN
2	na	sing	1:CYS24:SG	2:ZN1:ZN
3	na	sing	1:HIS37:NE2	2:ZN1:ZN
4	na	sing	1:HIS42:NE2	2:ZN1:ZN
5	na	sing	1:CYS53:SG	2:ZN1:ZN
6	na	sing	1:CYS56:SG	2:ZN1:ZN
7	na	sing	1:HIS69:NE2	2:ZN1:ZN
8	na	sing	1:HIS74:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Sparky ASSIGNMENT - CYANA structure calculations; run1: use 70% most intense peaks for initial fold, run2: refine initial fold using all peaks - water refinement with CNS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 82.4 %, Completeness: 72.3 %, Completeness (bb): 78.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.3 % (891 of 1232)	75.0 % (480 of 640)	67.7 % (323 of 477)	76.5 % (88 of 115)
Backbone	78.9 % (478 of 606)	79.9 % (163 of 204)	78.2 % (237 of 303)	78.8 % (78 of 99)
Sidechain	68.0 % (493 of 725)	72.7 % (317 of 436)	60.8 % (166 of 273)	62.5 % (10 of 16)
Aromatic	24.0 % (36 of 150)	41.3 % (31 of 75)	6.7 % (5 of 75)
Methyl	81.0 % (68 of 84)	81.0 % (34 of 42)	81.0 % (34 of 42)

1. hZHX1-ZNF

MAHHHHHHNQ QNKKVEGGYE CKYCTFQTPD LNMFTFHVDS EHPNVVLNSS YVCVECNFLT KRYDALSEHN LKYHPGEENF KLTMVKRNNQ TIFEQTINDL TF

Show sample condition

Sample

Temperature 293 (±0.0) K, pH 8.0 (±0.0)

#	Name	Isotope labeling	Type	Concentration
1	hZHX1-ZNF	[U-98% 13C; U-99% 15N]	protein	0.3 mM
2	NaPO4		buffer	30 mM
3	NaCl		salt	100 mM
4	ZnCl2		salt	50 mM
5	D2O		solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.74 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.74 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.28 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2GHF, Strand ID: A Detail

Release date

2006-04-04

Citation

The tandem zinc-finger region of human ZHX adopts a novel C2H2 zinc finger structure with a C-terminal extension
Wienk, H., Lammers, I., Hotze, A., Wu, J., Wechselberger, R.W., Owens, R., Stammers, D.K., Stuart, D., Kaptein, R., Folkers, G.E.
Biochemistry (2009), 48, 4431-4439, PubMed 19348505 , DOI 10.1021/bi9001997 , Abstract

Related entities 1. hZHX1-ZNF, : 1 : 9 : 18 entities Detail

Interaction partners 1. hZHX1-ZNF, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 10 experiments Detail

1 1H15N_HSQC