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BMRB: 7056

2GMO

1H, 13C, 15N assignments of an independently folded C-terminal domain of influenza polymerase subunit PB2

Authors

Boudet, J., Bougault, C.M., Simorre, J.

Assembly

DPDE

Entity

1. DPDE (polymer, Thiol state: not present), 83 monomers, 8972.145 × 2 Da Detail

GDPDESTSGV ESAVLRGFLI LGKEDRRYGP ALSINELSNL AKGEKANVLI GQGDVVLVMK RKRDSSILTD SQTATKRIRM AIN

Total weight

17944.29 Da

Max. entity weight

8972.145 Da

Source organism

Influenza A virus (A/Victoria/3/1975(H3N2))

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.9 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.9 % (903 of 932)	95.9 % (467 of 487)	98.3 % (352 of 358)	96.6 % (84 of 87)
Backbone	97.4 % (481 of 494)	95.9 % (165 of 172)	98.8 % (238 of 241)	96.3 % (78 of 81)
Sidechain	96.7 % (496 of 513)	95.9 % (302 of 315)	97.9 % (188 of 192)	100.0 % (6 of 6)
Aromatic	83.3 % (15 of 18)	100.0 % (9 of 9)	66.7 % (6 of 9)
Methyl	99.0 % (103 of 104)	98.1 % (51 of 52)	100.0 % (52 of 52)

1. DPDE-trans

GDPDESTSGV ESAVLRGFLI LGKEDRRYGP ALSINELSNL AKGEKANVLI GQGDVVLVMK RKRDSSILTD SQTATKRIRM AIN

Show sample condition

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2GMO, Strand ID: A Detail

Heteronucl. T₁

68 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 600 MHz, Temperature 283 (±0.5) K, pH 7.5 (±0.1) Detail

Heteronucl. T₂

69 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 600 MHz, Temperature 283 (±0.5) K, pH 7.5 (±0.1) Detail

Heteronucl. T_1ρ

70 T_1ρ values in 1 lists
Coherence S(+,-), Field strength (¹H) 600 MHz, Temperature 283 (±0.5) K, pH 7.5 (±0.1) Detail

Heteronucl. NOE

69 NOE values in 1 lists
Value type relative intensities, Field strength (¹H) 600 MHz, Temperature 283 (±0.5) K, pH 7.5 (±0.1) Detail

Heteronucl. T₁/T₂

68 T₁/T₂ values in 1 lists
Field strength (¹H) 600 MHz, Temperature 283 (±0.5) K, pH 7.5 (±0.1) Detail

Coupling constant

56 J values in 1 lists
Temperature 283 (±0.5) K, pH 7.5 (±0.1) Detail

Release date

2007-03-08

Citation

Structure and nuclear import function of the C-terminal domain of influenza virus polymerase PB2 subunit
Tarendeau, F., Boudet, J., Guiligay, D., Mas, P., Bougault, C.M., Boulo, S., Baudin, F., Ruigrok, R.W., Daigle, N., Ellenberg, J., Cusack, S., Simorre, J., Hart, D.J.
Nat. Struct. Mol. Biol. (2007), 14, 229-233, PubMed 17310249 , DOI 10.1038/nsmb1212 , Abstract

Related entities 1. DPDE, : 1 : 1 : 28 : 85 entities Detail

Interaction partners 1. DPDE, : 6 interactors Detail

More details for 6 interactors identified by 3 citations

Experiments performed 22 experiments Detail

1 1H15N_HSQC

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

2 HNCA

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

3 HN(CO)CA

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

4 HN(CA)CO

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

5 HNCACB

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

6 CBCA(CO)NH

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

7 H(CCO)NH

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

8 (H)C(CO)NH

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

9 H(C)CH-TOCSY

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

10 (H)CCH-TOCSY

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

11 1H13C_HSQC

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

12 methylselective-1H13C_HSQC

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

13 13C_NOESY_HSQC

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

14 15N_NOESY_HSQC

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

15 HNHA

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

16 1H15N_HSQC_t1

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

17 1H15N_HSQC_t1rho

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

18 1H15N_HSQC_t2

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

19 methylselective-13C_NOESY_HSQC

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

20 2D-1H1H-NOESY

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

21 15N_heteronuclear_{1H}-NOE

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

22 HNCO

Instrument

#1 Model Varian Inova600 (600 MHz)

#2 Model Varian Inova800 (800 MHz)

Sample #1

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Sample contains 75% of P3-trans form and 25% of P3-cis form at pH = 7.5 at 10C in H2O 90%/ D2O 10%

#	Name	Isotope labeling	Type	Concentration
1	DPDE-trans	[U-13C; U-15N]	protein	0.45 (±0.05) mM
2	DPDE-cis	[U-13C; U-15N]	protein	0.15 (±0.05) mM
3	sodium chloride		salt	200 mM
4	Tris pH = 7.5		buffer	50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 283 (±0.5) K, pH 7.5 (±0.1), Details Same sample as before in D2O 99.9%

#	Name	Isotope labeling	Type	Concentration
7	DPDE-trans	[U-95% 13C; U-90% 15N]	protein	0.45 (±0.05) mM
8	DPDE-cis	[U-95% 13C; U-90% 15N]	protein	0.15 (±0.05) mM
9	sodium chloride		salt	200 mM
10	Tris pH = 7.5		buffer	50 mM
11	D2O			99.9 %

nullKeywords ESPRIT, Influenza, PB2, RNA-polymerase

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Found 1 Document (4.708 seconds)

BMRB: 7056

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. DPDE, : 1 : 1 : 28 : 85 entities Detail

Interaction partners 1. DPDE, : 6 interactors Detail

Experiments performed 22 experiments Detail

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