BMRB: 7061

NMR structure of talin-PTB in complex with PIPKI

Authors

Qin, J., Kong, X., Wang, X., Misra, S.

Assembly

Talin-1

Entity

1. Talin-1 (polymer, Thiol state: all free), 106 monomers, 12210.06 Da Detail

LKTYGVSFFL VKEKMKGKNK LVPRLLGITK ECVMRVDEKT KEVIQEWSLT NIKRWAASPK SFTLDFGDYQ DGYYSVQTTE GEQIAQLIAG YIDIILKKKK SKDHFG

2. SWVXSPLH (polymer, Thiol state: not present), 8 monomers, 1068.075 Da Detail

SWVXSPLH

Total weight

13278.135 Da

Max. entity weight

12210.06 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

Distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 93.9 %, Completeness: 87.4 %, Completeness (bb): 79.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.4 % (1225 of 1401)	94.4 % (692 of 733)	76.6 % (420 of 548)	94.2 % (113 of 120)
Backbone	79.6 % (535 of 672)	93.9 % (217 of 231)	64.7 % (214 of 331)	94.5 % (104 of 110)
Sidechain	94.6 % (789 of 834)	94.6 % (475 of 502)	94.7 % (305 of 322)	90.0 % (9 of 10)
Aromatic	88.1 % (118 of 134)	88.1 % (59 of 67)	89.1 % (57 of 64)	66.7 % (2 of 3)
Methyl	96.8 % (122 of 126)	96.8 % (61 of 63)	96.8 % (61 of 63)

1. Talin-1

LKTYGVSFFL VKEKMKGKNK LVPRLLGITK ECVMRVDEKT KEVIQEWSLT NIKRWAASPK SFTLDFGDYQ DGYYSVQTTE GEQIAQLIAG YIDIILKKKK SKDHFG

2. SWVXSPLH

SWVXSPLH

Show sample condition

Sample

Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
1	Talin-1	[U-13C; U-15N]		1 mM
2	SWVXSPLH			1 mM
3	phosphate buffer			20 mM
4	Ca2+			5 mM
5	D2O			10 %
6	H2O			90 %

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Chem. Shift Complete2

Sequence coverage: 36.0 %, Completeness: 56.9 %, Completeness (bb): 55.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	56.9 % (1593 of 2802)	59.8 % (876 of 1466)	51.4 % (563 of 1096)	64.2 % (154 of 240)
Backbone	55.4 % (744 of 1344)	62.1 % (287 of 462)	47.6 % (315 of 662)	64.5 % (142 of 220)
Sidechain	59.0 % (984 of 1668)	58.7 % (589 of 1004)	59.5 % (383 of 644)	60.0 % (12 of 20)
Aromatic	50.0 % (134 of 268)	50.0 % (67 of 134)	50.0 % (64 of 128)	50.0 % (3 of 6)
Methyl	61.9 % (156 of 252)	61.9 % (78 of 126)	61.9 % (78 of 126)

1. Talin-1

LKTYGVSFFL VKEKMKGKNK LVPRLLGITK ECVMRVDEKT KEVIQEWSLT NIKRWAASPK SFTLDFGDYQ DGYYSVQTTE GEQIAQLIAG YIDIILKKKK SKDHFG

2. SWVXSPLH

SWVXSPLH

Show sample condition

Sample

Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
1	Talin-1	[U-13C; U-15N]		1 mM
2	SWVXSPLH			1 mM
3	phosphate buffer			20 mM
4	Ca2+			5 mM
5	D2O			10 %
6	H2O			90 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2G35, Strand ID: A, B Detail

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Release date

2008-07-16

Citation

Structural basis for the phosphorylation-regulated focal adhesion targeting of type Igamma phosphatidylinositol phosphate kinase (PIPKIgamma) by talin
Kong, X., Wang, X., Misra, S., Qin, J.
J. Mol. Biol. (2006), 359, 47-54, PubMed 16616931 , DOI 10.1016/j.jmb.2006.02.048 , Abstract

Related entities 1. Talin-1, : 4 : 2 : 1 : 64 entities Detail

Experiments performed 6 experiments Detail