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Found 1 Document (1.639 seconds)

BMRB: 7063

2G7J

Solution NMR structure of the putative cytoplasmic protein ygaC from Salmonella typhimurium. Northeast Structural Genomics target StR72.

Authors

Aramini, J.M., Swapna, G.V.T., Ramelot, T.A., Ho, C.K., Cunningham, K., Ma, L., Shetty, K., Xiao, R., Acton, T.B., Kennedy, M.A., Montelione, G.T.

Assembly

putative cytoplasmic protein

Entity

1. putative cytoplasmic protein (polymer, Thiol state: all free), 124 monomers, 14377.87 Da Detail

MYLRPDEVAR VLEKAGFTVD VVTNKTYGYR RGENYVYVNR EARMGRTALI IHPRLKDRSS SLADPASDIK TCDHYQNFPL YLGGETHEHY GIPHGFSSRI ALERYLNGLF GDEKTDLEHH HHHH

Formula weight

14377.87 Da

Source organism

Salmonella enterica subsp. enterica serovar Typhimurium

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 94.5 %, Completeness (bb): 96.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.5 % (1365 of 1445)	94.1 % (706 of 750)	94.2 % (537 of 570)	97.6 % (122 of 125)
Backbone	96.7 % (710 of 734)	95.7 % (242 of 253)	97.2 % (352 of 362)	97.5 % (116 of 119)
Sidechain	92.8 % (766 of 825)	93.4 % (464 of 497)	91.9 % (296 of 322)	100.0 % (6 of 6)
Aromatic	78.8 % (123 of 156)	84.6 % (66 of 78)	73.1 % (57 of 78)
Methyl	98.3 % (118 of 120)	98.3 % (59 of 60)	98.3 % (59 of 60)

1. putative cytoplasmic protein

MYLRPDEVAR VLEKAGFTVD VVTNKTYGYR RGENYVYVNR EARMGRTALI IHPRLKDRSS SLADPASDIK TCDHYQNFPL YLGGETHEHY GIPHGFSSRI ALERYLNGLF GDEKTDLEHH HHHH

Show sample condition

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		100 %
16	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
17	putative cytoplasmic protein	[U-5% 13C; U-15N]	1.07 mM
18	MES		20 mM
19	NaCl		100 mM
20	CaCl2		5 mM
21	DTT		10 mM
22	NaN3		0.02 %
23	D2O		5 %
24	H2O		95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2G7J, Strand ID: A Detail

Coupling constant

65 J values in 1 lists
Pressure 1 atm, Temperature 293 K, pH 6.5 Detail

Release date

2007-01-31

Citation

Solution NMR structure of the putative cytoplasmic protein ygaC from Salmonella typhimurium. Northeast Structural Genomics target StR72
Aramini, J.M., Swapna, G.V.T., Ramelot, T.A., Ho, C.K., Cunningham, K., Ma, L., Shetty, K., Xiao, R., Acton, T.B., Kennedy, M.A., Montelione, G.T.

Related entities 1. putative cytoplasmic protein, : 1 : 2 entities Detail

Experiments performed 9 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		100 %
16	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
17	putative cytoplasmic protein	[U-5% 13C; U-15N]	1.07 mM
18	MES		20 mM
19	NaCl		100 mM
20	CaCl2		5 mM
21	DTT		10 mM
22	NaN3		0.02 %
23	D2O		5 %
24	H2O		95 %

2 3D 13C-separated NOESY

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		100 %
16	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
17	putative cytoplasmic protein	[U-5% 13C; U-15N]	1.07 mM
18	MES		20 mM
19	NaCl		100 mM
20	CaCl2		5 mM
21	DTT		10 mM
22	NaN3		0.02 %
23	D2O		5 %
24	H2O		95 %

3 4D 13C-separated NOESY

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		100 %
16	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
17	putative cytoplasmic protein	[U-5% 13C; U-15N]	1.07 mM
18	MES		20 mM
19	NaCl		100 mM
20	CaCl2		5 mM
21	DTT		10 mM
22	NaN3		0.02 %
23	D2O		5 %
24	H2O		95 %

4 HNHA

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		100 %
16	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
17	putative cytoplasmic protein	[U-5% 13C; U-15N]	1.07 mM
18	MES		20 mM
19	NaCl		100 mM
20	CaCl2		5 mM
21	DTT		10 mM
22	NaN3		0.02 %
23	D2O		5 %
24	H2O		95 %

5 high resolution CH-HSQC (stereospecific assignment of Val/Leu methyls)

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		100 %
16	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
17	putative cytoplasmic protein	[U-5% 13C; U-15N]	1.07 mM
18	MES		20 mM
19	NaCl		100 mM
20	CaCl2		5 mM
21	DTT		10 mM
22	NaN3		0.02 %
23	D2O		5 %
24	H2O		95 %

6 backbone TR expts

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		100 %
16	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
17	putative cytoplasmic protein	[U-5% 13C; U-15N]	1.07 mM
18	MES		20 mM
19	NaCl		100 mM
20	CaCl2		5 mM
21	DTT		10 mM
22	NaN3		0.02 %
23	D2O		5 %
24	H2O		95 %

7 3D HCCH-COSY

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		100 %
16	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
17	putative cytoplasmic protein	[U-5% 13C; U-15N]	1.07 mM
18	MES		20 mM
19	NaCl		100 mM
20	CaCl2		5 mM
21	DTT		10 mM
22	NaN3		0.02 %
23	D2O		5 %
24	H2O		95 %

8 3D TOCSYs

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		100 %
16	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
17	putative cytoplasmic protein	[U-5% 13C; U-15N]	1.07 mM
18	MES		20 mM
19	NaCl		100 mM
20	CaCl2		5 mM
21	DTT		10 mM
22	NaN3		0.02 %
23	D2O		5 %
24	H2O		95 %

9 aromatic expts

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	putative cytoplasmic protein	[U-13C; U-15N]	1.12 mM
10	MES		20 mM
11	NaCl		100 mM
12	CaCl2		5 mM
13	DTT		10 mM
14	NaN3		0.02 %
15	D2O		100 %
16	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
17	putative cytoplasmic protein	[U-5% 13C; U-15N]	1.07 mM
18	MES		20 mM
19	NaCl		100 mM
20	CaCl2		5 mM
21	DTT		10 mM
22	NaN3		0.02 %
23	D2O		5 %
24	H2O		95 %

nullKeywords AUTOSTRUCTURE, NESG, NMR structure, Northeast Structural Genomics Consortium, Protein Structure Initiative, PSI, StR72, Structural Genomics

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Found 1 Document (1.639 seconds)

BMRB: 7063

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. putative cytoplasmic protein, : 1 : 2 entities Detail

Experiments performed 9 experiments Detail

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