BMRB: 7070

RRMs 1 and 2 of Prp24 from S. cerevisiae

Authors

Reiter, N.J., Lee, D., Wang, Y., Tonelli, M., Bahrami, A., Cornilescu, C.C., Butcher, S.E.

Assembly

monomer

Entity

1. monomer (polymer, Thiol state: all free), 161 monomers, 18598.33 Da Detail

MRELTTVLVK NLPKSYNQNK VYKYFKHCGP IIHVDVADSL KKNFRFARIE FARYDGALAA ITKTHKVVGQ NEIIVSHLTE CTLWMTNFPP SYTQRNIRDL LQDINVVALS IRLPSLRFNT SRRFAYIDVT SKEDARYCVE KLNGLKIEGY TLVTKVSNPL E

Formula weight

18598.33 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.2 %, Completeness (bb): 97.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.2 % (1765 of 1956)	88.8 % (903 of 1017)	90.5 % (695 of 768)	97.7 % (167 of 171)
Backbone	97.3 % (928 of 954)	97.2 % (312 of 321)	96.7 % (462 of 478)	99.4 % (154 of 155)
Sidechain	85.7 % (992 of 1158)	84.9 % (591 of 696)	87.0 % (388 of 446)	81.3 % (13 of 16)
Aromatic	64.8 % (105 of 162)	81.5 % (66 of 81)	47.5 % (38 of 80)	100.0 % (1 of 1)
Methyl	97.1 % (200 of 206)	96.1 % (99 of 103)	98.1 % (101 of 103)

1. PRP24 (RRM 1 and 2)

MRELTTVLVK NLPKSYNQNK VYKYFKHCGP IIHVDVADSL KKNFRFARIE FARYDGALAA ITKTHKVVGQ NEIIVSHLTE CTLWMTNFPP SYTQRNIRDL LQDINVVALS IRLPSLRFNT SRRFAYIDVT SKEDARYCVE KLNGLKIEGY TLVTKVSNPL E

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.1), Details 0.3 mM- 0.8 mM protein, 50 mM K(PO)4) 0.1 M KCL 2 mM DTT

#	Name	Isotope labeling	Type	Concentration
1	PRP24 (RRM 1 and 2)	[U-15N; U-13C]	protein	0.6 (±0.3) mM
2	K(PO)4)			50 mM
3	KCL			0.1 M
4	DTT			2 mM

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LACS Plot; CA

Referencing offset: -0.24 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.24 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.14 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 10 models in PDB: 2GO9, Strand ID: A Detail

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Release date

2006-12-03

Citation

Resonance assignments for the two N-terminal RNA recognition motifs (RRM) of the S. cerevisiae pre-mRNA processing protein Prp24
Reiter, N.J., Lee, D., Wang, Y., Tonelli, M., Bahrami, A., Cornilescu, C.C., Butcher, S.E.
J. Biomol. NMR (2006), 36 Suppl 1, 58-58, PubMed 17131032 , DOI 10.1007/s10858-006-9039-4 , Abstract

Related entities 1. monomer, : 1 : 7 : 123 entities Detail

Interaction partners 1. monomer, : 17 interactors Detail

Experiments performed 3 experiments Detail