BMRB: 7075

Assignment of conserved hypothetical protein pa2412

Authors

Lemak, A., Yee, A., Lukin, J., Semesi, A., Arrowsmith, C.H.

Assembly

conserved hypothetical protein pa2412

Entity

1. conserved hypothetical protein pa2412 (polymer, Thiol state: not present), 72 monomers, 8443.451 Da Detail

MTSVFDRDDI QFQVVVNHEE QYSIWPEYKE IPQGWRAAGK SGLKKDCLAY IEEVWTDMRP LSLRQHMDKA AG

Formula weight

8443.451 Da

Source organism

Pseudomonas aeruginosa

Exptl. method

NMR

Refine. method

molecular dynamics,

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.9 %, Completeness (bb): 91.1 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.9 % (745 of 867)	85.7 % (389 of 454)	85.1 % (285 of 335)	91.0 % (71 of 78)
Backbone	91.1 % (388 of 426)	91.0 % (132 of 145)	91.5 % (194 of 212)	89.9 % (62 of 69)
Sidechain	82.9 % (422 of 509)	83.2 % (257 of 309)	81.7 % (156 of 191)	100.0 % (9 of 9)
Aromatic	54.5 % (48 of 88)	54.5 % (24 of 44)	51.2 % (21 of 41)	100.0 % (3 of 3)
Methyl	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)

1. conserved hypothetical protein pa2412

MTSVFDRDDI QFQVVVNHEE QYSIWPEYKE IPQGWRAAGK SGLKKDCLAY IEEVWTDMRP LSLRQHMDKA AG

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	conserved hypothetical protein pa2412	[U-13C; U-15N]		0.6 mM
2	NaCl			450 mM
3	MOPS			10 mM

Hide sample codition

LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2GPF, Strand ID: A Detail

---

Release date

2006-10-19

Citation

Solution structure of pa2412 protein determined using ABACUS protocol
Lemak, A., Lukin, J., Yee, A., Semesi, A., Arrowsmith, C.H.

Related entities 1. conserved hypothetical protein pa2412, : 1 : 2 : 23 entities Detail

Experiments performed 11 experiments Detail