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BMRB: 7079

2GQB

Sequence specific assignment of a hypothetical protein RP2812 (NESG ID: RPT4) from Rhodopseudomonas palustris

Authors

Srisailam, S., Lukin, J.A., Yee, A., Lemak, A., Arrowsmith, C.H.

Assembly

RP2812

Entity

1. RP2812 (polymer, Thiol state: not present), 130 monomers, 13431.32 Da Detail

MSIFGKIMSA IFGDSAAASP GGAQAPATTG AAGTAPTAPQ PTAAPSIDVA PILDKAVKAK GEKLEWRTSI VDLMKALDID SSLSARKELA KELGYSGDMN DSASMNIWLH KQVMSKLVAN GGKLPPEIKH

Formula weight

13431.32 Da

Source organism

Rhodopseudomonas palustris

Exptl. method

NMR

Refine. method

Simulated Annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 92.6 %, Completeness (bb): 96.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.6 % (1317 of 1422)	94.0 % (691 of 735)	90.0 % (502 of 558)	96.1 % (124 of 129)
Backbone	96.2 % (733 of 762)	97.7 % (256 of 262)	94.7 % (359 of 379)	97.5 % (118 of 121)
Sidechain	89.0 % (693 of 779)	92.0 % (435 of 473)	84.6 % (252 of 298)	75.0 % (6 of 8)
Aromatic	70.0 % (42 of 60)	86.7 % (26 of 30)	50.0 % (14 of 28)	100.0 % (2 of 2)
Methyl	98.7 % (148 of 150)	100.0 % (75 of 75)	97.3 % (73 of 75)

1. conserved unknown protein RPA2825 from Rhodopseudomonas palustris

MSIFGKIMSA IFGDSAAASP GGAQAPATTG AAGTAPTAPQ PTAAPSIDVA PILDKAVKAK GEKLEWRTSI VDLMKALDID SSLSARKELA KELGYSGDMN DSASMNIWLH KQVMSKLVAN GGKLPPEIKH

Show sample condition

Sample

Temperature 298 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	conserved unknown protein RPA2825 from Rhodopseudomonas palustris	[U-15N; U-13C]	protein	1 (±0.2) mM
2	MOPS		buffer	10 (±0.05) mM
3	Sodium Chloride		salt	450 (±0.05) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl carbons	internal	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl carbons	internal	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl carbons	internal	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl carbons	internal	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2GQB, Strand ID: A Detail

Release date

2006-11-05

Citation

Sequence specific assignment of a hypothetical protein RP2812 (NESG ID: RPT4) from Rhodopseudomonas palustris
Srisailam, S., Lukin, J.A., Yee, A., Lemak, A., Arrowsmith, C.H.
J. Biomol. NMR

Related entities 1. RP2812, : 1 : 1 entities Detail

Experiments performed 10 experiments Detail

1 1H15N HSQC