BMRB: 7093

Chemical shift assignment of monomeric chorimate mutase from methanococcus jannaschii in a complex with a transition state analog

Authors

Vogeli, B.R., Pervushin, K., Vamvaca, K., Hilvert, D.

Assembly

mMjCM in complex with a TSA

Entity

1. monomeric chorismate mutase from methanococcus jannaschii (polymer, Thiol state: all free), 104 monomers, 12488.32 Da Detail

MIEKLAEIRK KIDEIDNKIL KARWPWAEKL IAERNSLAKD VAEIKNQLGI PINDPEREKY IYDRIRKLCK EHNVDENIGI KIFQRLIEHN KALQKQYLEE TLEH

2. TSA (non-polymer), 228.199 Da

Total weight

12716.52 Da

Max. entity weight

12488.32 Da

Source organism

Methanocaldococcus jannaschii

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.3 %, Completeness: 68.7 %, Completeness (bb): 68.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	68.7 % (912 of 1327)	73.4 % (520 of 708)	58.4 % (295 of 505)	85.1 % (97 of 114)
Backbone	68.0 % (420 of 618)	82.1 % (170 of 207)	53.5 % (166 of 310)	83.2 % (84 of 101)
Sidechain	70.3 % (570 of 811)	69.9 % (350 of 501)	69.7 % (207 of 297)	100.0 % (13 of 13)
Aromatic	82.9 % (58 of 70)	82.9 % (29 of 35)	81.8 % (27 of 33)	100.0 % (2 of 2)
Methyl	80.6 % (100 of 124)	82.3 % (51 of 62)	79.0 % (49 of 62)

1. monomeric chorismate mutase from methanococcus jannaschii

MIEKLAEIRK KIDEIDNKIL KARWPWAEKL IAERNSLAKD VAEIKNQLGI PINDPEREKY IYDRIRKLCK EHNVDENIGI KIFQRLIEHN KALQKQYLEE TLEH

Show sample condition

Sample

Temperature 293 (±0) K, pH 6.5 (±0), Details mMjCM in complex with TSA low concentration

#	Name	Isotope labeling	Type	Concentration
1	mMjCM in complex with TSA	[U-15N]		0.07 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 2GTV, Strand ID: X Detail

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Release date

2007-11-20

Citation

Structure and dynamics of a molten globular enzyme
Pervushin, K., Vamvaca, K., Vogeli, B., Hilvert, D.
Nat. Struct. Mol. Biol. (2007), 14, 1202-1206, PubMed 17994104 , DOI 10.1038/nsmb1325 , Abstract

Related entities 1. monomeric chorismate mutase from methanococcus jannaschii, : 1 : 17 entities Detail

Experiments performed 1 experiments Detail