BMRB: 7095

Solution structure of BC059385 from Homo sapiens

Authors

Volkman, B.F., de la Cruz, N.B., Lytle, B.L., Peterson, F.C.

Assembly

Ubiquitin-like protein 3

Entity

1. Ubiquitin-like protein 3 (polymer, Thiol state: all free), 125 monomers, 14147.85 Da Detail

GHHHHHHLES SNVPADMINL RLILVSGKTK EFLFSPNDSA SDIAKHVYDN WPMDWEEEQV SSPNILRLIY QGRFLHGNVT LGALKLPFGK TTVMHLVARE TLPEPNSQGQ RNREKTGESN CCVIL

Formula weight

14147.85 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

AUTOMATED METHODS WERE USED FOR BACKBONE CHEMICAL SHIFT ASSIGNMENT AND ITERATIVE NOE REFINEMENT.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 84.0 %, Completeness: 73.3 %, Completeness (bb): 77.2 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	73.3 % (1072 of 1462)	73.5 % (559 of 761)	73.6 % (418 of 568)	71.4 % (95 of 133)
Backbone	77.2 % (568 of 736)	78.1 % (196 of 251)	76.0 % (279 of 367)	78.8 % (93 of 118)
Sidechain	70.6 % (595 of 843)	71.2 % (363 of 510)	72.3 % (230 of 318)	13.3 % (2 of 15)
Aromatic	35.3 % (41 of 116)	43.1 % (25 of 58)	28.6 % (16 of 56)	0.0 % (0 of 2)
Methyl	82.8 % (111 of 134)	82.1 % (55 of 67)	83.6 % (56 of 67)

1. Ubiquitin-like protein 3

GHHHHHHLES SNVPADMINL RLILVSGKTK EFLFSPNDSA SDIAKHVYDN WPMDWEEEQV SSPNILRLIY QGRFLHGNVT LGALKLPFGK TTVMHLVARE TLPEPNSQGQ RNREKTGESN CCVIL

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.0

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin-like protein 3	[U-15N; U-13C]		0.5 mM
2	deuterated sodium acetate			10 mM
3	NaCl			100 mM
4	Dithiothreitol			2 mM
5	H2O			90 %
6	D2O			10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2GOW, Strand ID: A Detail

---

Release date

2007-09-16

Citation

Solution structure of a membrane-anchored ubiquitin-fold (MUB) protein from Homo sapiens
de la Cruz, N.B., Peterson, F.C., Lytle, B.L., Volkman, B.F.
Protein Sci. (2007), 16, 1479-1484, PubMed 17567738 , DOI 10.1110/ps.072834007 , Abstract

Related entities 1. Ubiquitin-like protein 3, : 1 : 2 : 16 entities Detail

Experiments performed 3 experiments Detail